| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 257,873 | A→G | 24.6% | K15K (AAA→AAG) | crl → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 257,873 | 0 | A | G | 24.6% | 100.8 / 36.3 | 57 | K15K (AAA→AAG) | crl | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (24/19); new base G (6/8); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCT > W3110S.gb/257803‑257941 | cAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCaaaaaa < 1:2152595/71‑1 (MQ=255) gTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAAt > 1:2913965/1‑70 (MQ=255) ttGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAAttt < 1:849580/71‑1 (MQ=255) tGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAAttt > 1:2765204/1‑70 (MQ=255) gCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAAttt > 1:4848915/1‑69 (MQ=255) tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGc < 1:2643783/71‑1 (MQ=255) acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCAc > 1:4315255/1‑71 (MQ=255) acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCAc > 1:29592/1‑71 (MQ=255) acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCAc > 1:1347119/1‑71 (MQ=255) acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc > 1:4442179/1‑71 (MQ=255) acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc < 1:4436345/71‑1 (MQ=255) acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc < 1:2971650/71‑1 (MQ=255) acaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc < 1:1577827/68‑1 (MQ=255) aGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTa > 1:2028349/1‑68 (MQ=255) gaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTAc < 1:871663/57‑1 (MQ=255) aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCg < 1:1111752/69‑1 (MQ=255) aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGta < 1:1805761/71‑1 (MQ=255) aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGta < 1:1322813/71‑1 (MQ=255) aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGtata > 1:4814511/1‑71 (MQ=255) aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGtata > 1:420628/1‑71 (MQ=255) aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGtata > 1:2583770/1‑71 (MQ=255) aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGtata > 1:2024303/1‑71 (MQ=255) cAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCaaaaaa > 1:1906928/1‑47 (MQ=255) aCGTTACCGAGTGGACACCCGGAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTg < 1:712350/70‑1 (MQ=255) aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAc > 1:1433922/1‑52 (MQ=255) gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAg > 1:1143737/1‑71 (MQ=255) ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTAc < 1:4474392/44‑1 (MQ=255) ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGc < 1:8082/47‑1 (MQ=255) aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGt < 1:3508095/70‑1 (MQ=255) aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCa > 1:1639980/1‑46 (MQ=255) aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGt > 1:4333999/1‑64 (MQ=255) aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAgg > 1:1766416/1‑69 (MQ=255) aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGt < 1:453274/70‑1 (MQ=255) ccGAGTGGACACCCGAAGAGCAGATTGATCaaaaaa < 1:5027164/36‑1 (MQ=255) ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTa < 1:3360725/70‑1 (MQ=255) gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCaa < 1:3424255/70‑1 (MQ=255) acacCCGAAGAGCAGATTGATCAAAAAGTTTACCg < 1:3775333/35‑1 (MQ=255) cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGtata > 1:2892382/1‑49 (MQ=255) cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGa < 1:1090905/71‑1 (MQ=255) cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGa < 1:1841357/54‑1 (MQ=255) cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAg > 1:4315422/1‑54 (MQ=255) aaGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCg < 1:4394868/71‑1 (MQ=255) gagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGAt > 1:855837/1‑71 (MQ=255) cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGt > 1:1330242/1‑43 (MQ=255) cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGt > 1:4399928/1‑43 (MQ=255) aGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGAt < 1:1346279/60‑1 (MQ=255) aGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGAt < 1:575862/60‑1 (MQ=255) gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt > 1:534152/1‑71 (MQ=255) gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt > 1:1681172/1‑71 (MQ=255) aTTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAg < 1:2077916/44‑1 (MQ=255) aTTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGa < 1:471685/57‑1 (MQ=255) aTTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGa < 1:2965618/57‑1 (MQ=255) aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGt < 1:1649728/50‑1 (MQ=255) tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTaa > 1:3665616/1‑46 (MQ=255) tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTaa > 1:2262668/1‑46 (MQ=255) tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGc > 1:1459890/1‑50 (MQ=255) tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTc > 1:4051291/1‑71 (MQ=255) tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTc > 1:283536/1‑71 (MQ=255) aTCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGa < 1:1370589/71‑1 (MQ=255) cAAAAAATTTACCGCACTAGGCCCGTATATTCGTg > 1:333928/1‑35 (MQ=255) aaaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTg < 1:1596701/71‑1 (MQ=255) aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGa > 1:1116871/1‑68 (MQ=255) aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGAt > 1:5016235/1‑69 (MQ=255) aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTg < 1:3940715/70‑1 (MQ=255) aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTc < 1:1107880/65‑1 (MQ=255) aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTc < 1:3693695/65‑1 (MQ=255) aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGt < 1:355908/71‑1 (MQ=255) aaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCt < 1:534452/71‑1 (MQ=255) | CAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCT > W3110S.gb/257803‑257941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |