| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,485,691 | T→C | 15.1% | R703R (CGT→CGC) | ppc → | phosphoenolpyruvate carboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,485,691 | 0 | T | C | 15.1% | 78.3 / 7.9 | 33 | R703R (CGT→CGC) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base T (14/14); new base C (3/2); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
ACTTCCGCTCCGCTACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCAAAACCGTCTGA > W3110S.gb/3485624‑3485761 | aCTTCCGCTCCGCTACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAAcgccgc > 1:4592662/1‑71 (MQ=255) cTTCCGCTCCGCTACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGcc > 1:1577716/1‑71 (MQ=255) cTTCCGCTCCGCTACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGcc > 1:4084631/1‑71 (MQ=255) cTTCCGCTCCGCTACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGcc > 1:4043643/1‑71 (MQ=255) ccgctccgctACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCa < 1:3444885/70‑1 (MQ=255) ccgctccgctACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCa < 1:3438926/70‑1 (MQ=255) ccgctccgctACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGCCGccc < 1:5116675/69‑1 (MQ=255) ctACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGCCGCCCAACCggcggc > 1:4477438/1‑71 (MQ=255) ctACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGCCGCCCAACCggcggc > 1:3047278/1‑71 (MQ=255) cGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTc < 1:3539932/71‑1 (MQ=255) cGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTc < 1:3533805/71‑1 (MQ=255) ggAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGt < 1:865792/71‑1 (MQ=255) aCAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCAc < 1:2978920/71‑1 (MQ=255) aCAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCAc < 1:3924421/71‑1 (MQ=255) aCAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCAc < 1:1495854/71‑1 (MQ=255) cAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTc < 1:1598409/63‑1 (MQ=255) aCTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCCCTAcgcg > 1:4748936/1‑71 (MQ=255) tGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACgcgc > 1:782542/1‑70 (MQ=255) tGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACgcgc > 1:2760396/1‑70 (MQ=255) gggCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGgcggcg < 1:4630686/53‑1 (MQ=255) aaaCTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGt < 1:3114383/55‑1 (MQ=255) aaaCTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTcc < 1:1607899/71‑1 (MQ=255) ccGTTGGGTTCACGTCCGGCGAAACGCCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTgg < 1:3089197/69‑1 (MQ=255) ggTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGc < 1:4749436/71‑1 (MQ=255) ggTTCACGTCCGGCGAAACGCCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCg > 1:4333825/1‑60 (MQ=255) cACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGc > 1:4526917/1‑67 (MQ=255) aCGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTg > 1:3440241/1‑57 (MQ=255) aCGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGa > 1:1291185/1‑71 (MQ=255) gCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCaaaa > 1:4697208/1‑71 (MQ=255) gCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCaaaa > 1:1919129/1‑71 (MQ=255) aCGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCAAAACCGTc > 1:4722652/1‑71 (MQ=255) aCGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCAAAACCGTc < 1:3302900/71‑1 (MQ=255) aCGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCAAAACCGTc > 1:1463760/1‑71 (MQ=255) aCGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCAAAACCGTc > 1:743342/1‑71 (MQ=255) tCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCAAAACCGTCTGa < 1:4695663/71‑1 (MQ=255) | ACTTCCGCTCCGCTACGCCGGAACAAGAACTGGGCAAACTGCCGTTGGGTTCACGTCCGGCGAAACGTCGCCCAACCGGCGGCGTCGAGTCACTACGCGCCATTCCGTGGATCTTCGCCTGGACGCAAAACCGTCTGA > W3110S.gb/3485624‑3485761 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |