| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,497,165 | G→A | 40.4% | A65T (GCG→ACG) | fsaB → | fructose‑6‑phosphate aldolase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,497,165 | 0 | G | A | 40.4% | 37.9 / 57.5 | 52 | A65T (GCG→ACG) | fsaB | fructose‑6‑phosphate aldolase 2 |
| Reads supporting (aligned to +/- strand): ref base G (14/17); new base A (13/8); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGAA > W3110S.gb/3497097‑3497226 | tgctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg < 1:647494/71‑1 (MQ=255) gctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg > 1:1831749/1‑70 (MQ=255) gctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcgcg > 1:4660771/1‑70 (MQ=255) ctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcacg > 1:410894/1‑69 (MQ=255) ctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGAcacg > 1:2386445/1‑69 (MQ=255) ctgcCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACacgc < 1:3267600/70‑1 (MQ=255) ccGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAggg < 1:2958770/71‑1 (MQ=255) ccGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAggg > 1:4401581/1‑71 (MQ=255) tCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACgcgc < 1:4539237/62‑1 (MQ=255) tGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggtg > 1:1371395/1‑71 (MQ=255) tGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggtg > 1:690578/1‑71 (MQ=255) tGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTAAGACCATGAGCCGCGACGCGCAGGGGAtggtg > 1:1459228/1‑71 (MQ=255) aaaaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGa < 1:4532350/63‑1 (MQ=255) aaaaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGa < 1:4185235/63‑1 (MQ=255) aaaGCGATTGGTGATGAGGGCATTCTGTTTTCTCAGACCATGAGCCGCGACACGCAggg > 1:4525795/1‑59 (MQ=255) aaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaaga < 1:4331348/71‑1 (MQ=255) aaaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaaga < 1:2653577/71‑1 (MQ=255) aaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaagaa < 1:3746465/71‑1 (MQ=255) aaGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaagaa < 1:3668700/71‑1 (MQ=255) gCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGaagaag < 1:4466940/70‑1 (MQ=255) gCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAg > 1:2905283/1‑54 (MQ=255) gCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAg > 1:1595086/1‑54 (MQ=255) aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcg < 1:1447993/69‑1 (MQ=255) aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcg < 1:3715493/69‑1 (MQ=255) aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAgaagcgaa < 1:1470337/71‑1 (MQ=255) aTTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAgaagcg < 1:1907774/69‑1 (MQ=255) ttGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAgaagcg < 1:4830904/68‑1 (MQ=255) ggTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtgg > 1:2706960/1‑55 (MQ=255) gTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAgcgc > 1:2182763/1‑71 (MQ=255) atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGgaagaa < 1:3474548/59‑1 (MQ=255) atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGAt < 1:317126/49‑1 (MQ=255) atgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGAt < 1:1012087/49‑1 (MQ=255) tgaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGa < 1:979936/47‑1 (MQ=255) gaGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAgggg > 1:3396288/1‑45 (MQ=255) ggCATTCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGa < 1:3345035/71‑1 (MQ=255) tCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAgaagc < 1:2711678/51‑1 (MQ=255) tCTGTTTGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTa > 1:3586395/1‑71 (MQ=255) cTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGAtggt > 1:2948846/1‑41 (MQ=255) cTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTAt > 1:3333851/1‑71 (MQ=255) cTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTAt > 1:4696410/1‑71 (MQ=255) tGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGaagcgaagcg > 1:1823145/1‑55 (MQ=255) tttGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGcc < 1:928884/55‑1 (MQ=255) tGCTCAGACCATGAGCCGCGACGCGCAGGGGAtgg > 1:2227282/1‑35 (MQ=255) tGCTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaagaa > 1:3583167/1‑43 (MQ=255) cTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa > 1:4509795/1‑38 (MQ=255) cTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa > 1:902019/1‑38 (MQ=255) cTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa > 1:680732/1‑38 (MQ=255) cTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa > 1:3136749/1‑38 (MQ=255) cTCAGACCATGAGCCGCGACACGCAGGGGATGGTGgaa > 1:3170008/1‑38 (MQ=255) tCAGACCATGAGCCGCGACACGCAGGGGATGGTGGAAgaagcga < 1:4190563/44‑1 (MQ=255) aGACCATGAGCCGCGACGCGCAGTGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTAtt < 1:2967375/71‑1 (MQ=255) aGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGt > 1:4375196/1‑68 (MQ=255) gACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATtg < 1:2205474/71‑1 (MQ=255) ccATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATtgt < 1:3826693/70‑1 (MQ=255) cATGAGCCGCGACACGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGa > 1:3231205/1‑51 (MQ=255) tGAGCCGCGACACGCAGGGGATGGTGGAAgaagcga > 1:3729208/1‑36 (MQ=255) tGAGCCGCGACACGCAGGGGATGGTGGAAgaagcga > 1:1708531/1‑36 (MQ=255) aGCCGCGACGCGCAGGGGATGGTGGAAgaagcgaag > 1:212747/1‑36 (MQ=255) gCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGaa < 1:3164973/70‑1 (MQ=255) | TGCTGCCGCGTCTGCAAAAAGCGATTGGTGATGAGGGCATTCTGTTTGCTCAGACCATGAGCCGCGACGCGCAGGGGATGGTGGAAGAAGCGAAGCGCCTGCGCGACGCTATTCCGGGTATTGTGGTGAA > W3110S.gb/3497097‑3497226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |