| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,502,074 | T→C | 28.8% | E258G (GAA→GGA) | katG ← | catalase/hydroperoxidase HPI(I) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,502,074 | 0 | T | C | 28.8% | 82.8 / 46.3 | 59 | E258G (GAA→GGA) | katG | catalase/hydroperoxidase HPI(I) |
| Reads supporting (aligned to +/- strand): ref base T (22/20); new base C (7/10); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01 | |||||||||||
CATTTGATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCGGTTCGCCGCT > W3110S.gb/3502006‑3502144 | cATTTGATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTttct < 1:1035058/71‑1 (MQ=255)cATTTGATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTttct > 1:4245014/1‑71 (MQ=255) tttGATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTttcttc > 1:4149348/1‑71 (MQ=255) gATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTtcgt < 1:3117681/70‑1 (MQ=255) gATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTtcgt < 1:3443471/70‑1 (MQ=255) gATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTtcgt < 1:3897427/70‑1 (MQ=255) gATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTtcgt < 1:396550/70‑1 (MQ=255) tCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGtt < 1:557118/70‑1 (MQ=255) tCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTc < 1:2200269/71‑1 (MQ=255) tCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTCCTTCGTCGtt < 1:3552703/70‑1 (MQ=255) ggACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCa > 1:2604683/1‑70 (MQ=255) ggACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTCCTTTGTCGTTCAt > 1:2275828/1‑71 (MQ=255) aCCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTCCTTCGTCGTTCATGc > 1:2651880/1‑71 (MQ=255) ccGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTttctt > 1:887559/1‑58 (MQ=255) cGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGccc < 1:280246/71‑1 (MQ=255) gggTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGcc > 1:1855380/1‑70 (MQ=255) ggTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCg < 1:895705/70‑1 (MQ=255) ggTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCg < 1:1025109/70‑1 (MQ=255) tttACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAgg > 1:524449/1‑71 (MQ=255) tttACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAgg > 1:807900/1‑71 (MQ=255) ttACCCAGCGTATGACCACCGGCAATCAGCGCCACGGTTTCTTCGTCGTTCa > 1:186017/1‑52 (MQ=255) ttACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCa > 1:4848375/1‑52 (MQ=255) ttACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAgct > 1:992846/1‑69 (MQ=255) ttACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTCCTTCGTCGTTCATGCCCATGTTGCCGAAgg < 1:336715/70‑1 (MQ=255) cccAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTcgc < 1:1780338/71‑1 (MQ=255) aGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCa > 1:4919149/1‑46 (MQ=255) aGCGTATGACCACCCGCAATCAGCGCCACGGTTCCTTCGTCGTTCATGCCCATGTTGCCGAAGGTcgcgcg < 1:2419573/71‑1 (MQ=255) gCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGccc < 1:2256298/50‑1 (MQ=255) cGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGa > 1:1662719/1‑71 (MQ=255) gTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGa > 1:2520136/1‑70 (MQ=255) gTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGa > 1:1392579/1‑70 (MQ=255) tATGACCACCCGCAATCAGCGCCACGGTTCCTTCGTCGtt < 1:626983/40‑1 (MQ=255) tATGACCACCCGCAATCAGCGCCACGGTTCCTTCGTCGtt < 1:2583735/40‑1 (MQ=255) tGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAgc > 1:4721288/1‑71 (MQ=255) tGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAgc > 1:3170768/1‑71 (MQ=255) tGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAg < 1:1204028/70‑1 (MQ=255) tGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAg < 1:3284656/70‑1 (MQ=255) tGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAg < 1:3526618/70‑1 (MQ=255) tGACCACCCGCAATCAGCGCCACGGTTCCTTCGTCGTTCATGCCCATGTTGCCGAAGGTc > 1:965603/1‑60 (MQ=255) gACCACCCGCAATCAGCGCCACGGTTCCTTCGTCttt < 1:201982/37‑1 (MQ=37) gACCACCCGCAATCAGCGCCACGGTTCCTTCGTCGtt < 1:3631382/37‑1 (MQ=255) ccaccCGCAATCAGCGCCACGGTTCCTTCGTCGTTCa > 1:4427999/1‑37 (MQ=255) ccaccCGCAATCAGCGCCACGGTTCCTTCGTCGTTCa > 1:2220315/1‑37 (MQ=255) ccaccCGCAATCAGCGCCACGGTTCCTTCGTCGTTCa > 1:2015283/1‑37 (MQ=255) ccGCAATCAGCGCCACGGTTCCTTCGTCGTTCATGCCCa < 1:4459707/39‑1 (MQ=255) tCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCg < 1:2307776/41‑1 (MQ=255) tCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCg < 1:4837301/41‑1 (MQ=255) tCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCg < 1:1442970/41‑1 (MQ=255) tCAGCGCCACGGTTCCTTCGTCGTTCATGCCCATGTTGCCGa < 1:2461428/42‑1 (MQ=255) tCAGCGCCACGGTTCCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGaa < 1:5077409/71‑1 (MQ=255) aGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGaa > 1:4098791/1‑41 (MQ=255) aGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAg > 1:3377251/1‑42 (MQ=255) aGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAg > 1:312253/1‑42 (MQ=255) aGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTcgcg > 1:2929432/1‑48 (MQ=255) aGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTcgcg > 1:237870/1‑48 (MQ=255) aGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAg > 1:2816007/1‑71 (MQ=255) aGCGCCACGGTTCCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGaaa > 1:2514573/1‑70 (MQ=255) cACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCGGtt > 1:4645824/1‑71 (MQ=255) cACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCGGtt > 1:3060699/1‑71 (MQ=255) aCGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAgct < 1:1443291/51‑1 (MQ=255) aCGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAgct < 1:2563603/51‑1 (MQ=255) aCGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAgct < 1:1082161/51‑1 (MQ=255) aCGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCGGTTc > 1:410715/1‑71 (MQ=255) tttCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGa < 1:3959507/42‑1 (MQ=255) tttCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGa < 1:2207442/42‑1 (MQ=255) tttCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCgg < 1:4526560/64‑1 (MQ=255) tttCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCgg < 1:1813559/64‑1 (MQ=255) tttCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCgg < 1:513396/64‑1 (MQ=255) tttCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCGGTTcgccg > 1:2987917/1‑71 (MQ=255) ttcttcGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAg > 1:5106252/1‑44 (MQ=255) ttcttcGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAg > 1:933993/1‑44 (MQ=255) tcttcGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCGGTTCGCCGCt > 1:1966203/1‑71 (MQ=255) | CATTTGATGTCGGACCGGCACCGTGGGTTTTACCCAGCGTATGACCACCCGCAATCAGCGCCACGGTTTCTTCGTCGTTCATGCCCATGTTGCCGAAGGTCGCGCGGATAGCTGCTGCCGCAGAAAGCGGTTCGCCGCT > W3110S.gb/3502006‑3502144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |