| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,516,285 | T→C | 33.4% | intergenic (+20/‑47) | hslU → / → menA | molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,516,285 | 0 | T | C | 33.4% | 81.9 / 68.2 | 73 | intergenic (+20/‑47) | hslU/menA | molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (22/26); new base C (12/12); total (35/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGCCG > W3110S.gb/3516219‑3516354 | tGGATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCAtt > 1:247684/1‑71 (MQ=255) tGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGttt < 1:126028/71‑1 (MQ=255) tGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGt > 1:1718703/1‑69 (MQ=255) cGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa < 1:2336137/71‑1 (MQ=255) gTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa > 1:3890022/1‑70 (MQ=255) ttGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGAt < 1:304900/70‑1 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTg > 1:4109401/1‑70 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:872844/71‑1 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:1380852/71‑1 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:1109249/71‑1 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTg < 1:2575397/70‑1 (MQ=255) aGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGaa < 1:2367378/71‑1 (MQ=255) gATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTcaccat < 1:5078143/53‑1 (MQ=255) aTGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGaa < 1:1281655/69‑1 (MQ=255) gAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa > 1:1833530/1‑57 (MQ=255) gAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGc > 1:1331875/1‑71 (MQ=255) gAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCAATGTTTGATGGGGCTGAAAg > 1:3055430/1‑69 (MQ=255) aaGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGcc > 1:865363/1‑71 (MQ=255) aaGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGcc > 1:683882/1‑71 (MQ=255) aTCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:4658135/62‑1 (MQ=255) tCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGTGGCTGAAAGGCCCCAt > 1:4563414/1‑71 (MQ=255) tCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAt > 1:4483400/1‑71 (MQ=255) tCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCAt > 1:1433229/1‑71 (MQ=255) cTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATggg > 1:1232261/1‑55 (MQ=255) cTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGttt > 1:2926804/1‑49 (MQ=255) cTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGaa > 1:1588153/1‑61 (MQ=255) cTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCAACATTGTTTGATGGGGCTGaaa > 1:1598516/1‑62 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:5026989/70‑1 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:4994018/70‑1 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt > 1:1698703/1‑70 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:2604707/70‑1 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:2735893/70‑1 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:314509/70‑1 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:4689024/70‑1 (MQ=255) gTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTg < 1:3320913/71‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCATCATTGtt < 1:1198145/40‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCATCATTGtt < 1:832832/40‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTg < 1:3486232/42‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGa < 1:4455130/52‑1 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTg > 1:2844131/1‑40 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa > 1:1283242/1‑50 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCAtt > 1:1187239/1‑61 (MQ=255) cTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtat < 1:3143659/71‑1 (MQ=255) tataATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:4606689/57‑1 (MQ=255) tataATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:339956/57‑1 (MQ=255) tataATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGta < 1:2295912/69‑1 (MQ=255) ataATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtatta > 1:3114202/1‑71 (MQ=255) taATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTAt < 1:1424712/58‑1 (MQ=255) taATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtattat > 1:2823689/1‑71 (MQ=255) taATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtattat > 1:2783705/1‑71 (MQ=255) taATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtattat > 1:3222511/1‑71 (MQ=255) taATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtatta > 1:872443/1‑70 (MQ=255) aTCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:71186/40‑1 (MQ=255) tCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:1842359/39‑1 (MQ=255) gcgTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTg < 1:941834/71‑1 (MQ=255) tCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaac > 1:2618323/1‑70 (MQ=255) tCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaac > 1:368083/1‑70 (MQ=255) tCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaac > 1:4676374/1‑70 (MQ=255) cAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGta > 1:3265075/1‑57 (MQ=255) aaTCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtatt < 1:2274414/58‑1 (MQ=255) cATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTa > 1:4362612/1‑43 (MQ=255) aTTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAAt < 1:2985042/71‑1 (MQ=255) aTTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTgg > 1:3061317/1‑46 (MQ=255) aTTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTgg > 1:5022776/1‑46 (MQ=255) aTTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAAt < 1:1656439/71‑1 (MQ=255) aTTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAAt < 1:1650372/71‑1 (MQ=255) ttCATCCTTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGcgcg > 1:658677/1‑47 (MQ=255) ttCATCATTGTTTGATGGGGCTGAAAGGCCCCAttttt < 1:1789150/38‑1 (MQ=255) ttCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTa < 1:4988403/39‑1 (MQ=255) ttCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGcgcg > 1:1156516/1‑47 (MQ=255) ttCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGcgcg > 1:1061735/1‑47 (MQ=255) ttCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTTGCGCGTATTATGACTGAACAACAAATTa < 1:4499039/70‑1 (MQ=255) ttCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTa < 1:1997396/70‑1 (MQ=255) ttCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTa < 1:1102120/70‑1 (MQ=255) tcatcaTTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAg < 1:1598573/70‑1 (MQ=255) catcatTGTTTTATGGGGCTGAAAGGCCCCAtttt < 1:105889/35‑1 (MQ=255) catcatTGTTTGATGGGGCTGAAAGGCCCCAtttt < 1:5064267/35‑1 (MQ=255) catcatTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGcc > 1:1556242/1‑71 (MQ=255) atcatTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGCCg < 1:1612762/71‑1 (MQ=255) tcatTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGCCg > 1:1374598/1‑70 (MQ=255) | TGGATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTAGCCG > W3110S.gb/3516219‑3516354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |