| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,521,572 | G→A | 37.5% | L157L (CTG→CTA) | glpX → | fructose 1,6‑bisphosphatase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,521,572 | 0 | G | A | 37.5% | 51.5 / 58.2 | 56 | L157L (CTG→CTA) | glpX | fructose 1,6‑bisphosphatase II |
| Reads supporting (aligned to +/- strand): ref base G (16/19); new base A (8/13); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTACGC > W3110S.gb/3521513‑3521641 | ccGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACg < 1:3712222/69‑1 (MQ=255) cTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCt < 1:2360532/71‑1 (MQ=255) ggATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTa < 1:1363008/71‑1 (MQ=255) ggATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTa < 1:2501082/71‑1 (MQ=255) gATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaa < 1:3592669/71‑1 (MQ=255) aTAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaaa > 1:3215265/1‑71 (MQ=255) aTAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaaa > 1:2161743/1‑71 (MQ=255) aTAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaaa > 1:514395/1‑71 (MQ=255) tAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAc > 1:1400806/1‑71 (MQ=255) tAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAAc < 1:4818247/71‑1 (MQ=255) ccTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccac < 1:3741495/71‑1 (MQ=255) ccTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccac < 1:4253426/71‑1 (MQ=255) ccTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccac < 1:1789239/71‑1 (MQ=255) cTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAAccacg > 1:2558777/1‑71 (MQ=255) gcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgccac < 1:1559694/71‑1 (MQ=255) aTGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccac < 1:2787724/63‑1 (MQ=255) aTGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccac < 1:515216/63‑1 (MQ=255) tGTAGCGGCCGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTaa < 1:157507/57‑1 (MQ=255) tAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTg > 1:3395190/1‑35 (MQ=255) tAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGa > 1:1058638/1‑36 (MQ=255) tAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGcc > 1:86956/1‑71 (MQ=255) tAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGcc > 1:4219587/1‑71 (MQ=255) ggcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTa > 1:4256550/1‑50 (MQ=255) gcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAAc < 1:295007/38‑1 (MQ=255) cggcgCTCGGCAAACCGTTGAGCGAACTGACTGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATc < 1:3092228/71‑1 (MQ=255) cTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAAcc < 1:4959025/47‑1 (MQ=255) tCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGa < 1:1998634/70‑1 (MQ=255) cGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGaaa > 1:1435282/1‑71 (MQ=255) ggCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAAt > 1:3722475/1‑71 (MQ=255) ggCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAAt > 1:3674026/1‑71 (MQ=255) gCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg < 1:4118789/71‑1 (MQ=255) gCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg < 1:964238/71‑1 (MQ=255) cAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg > 1:3575324/1‑70 (MQ=255) cAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg > 1:729648/1‑70 (MQ=255) aaaCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg > 1:2701391/1‑69 (MQ=255) aaCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt < 1:205764/62‑1 (MQ=255) aCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcagc < 1:2687301/71‑1 (MQ=255) aCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcagc < 1:1258290/71‑1 (MQ=255) aCCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcagc < 1:3377123/71‑1 (MQ=255) aCCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcag > 1:4877483/1‑70 (MQ=255) tGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGt < 1:2850684/49‑1 (MQ=255) tGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTc > 1:593538/1‑71 (MQ=255) gAGCGAACTAACGGTAACGATTCTGGCTAAAccacgccac < 1:4159574/40‑1 (MQ=255) gAGCGAACTAACGGTAACGATTCTGGCTAAAccacgccac < 1:4539824/40‑1 (MQ=255) gAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGa > 1:2070174/1‑42 (MQ=255) gAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGa > 1:2064291/1‑42 (MQ=255) cGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCg > 1:189774/1‑71 (MQ=255) cGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCg < 1:2860542/71‑1 (MQ=255) cGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCg < 1:3531641/71‑1 (MQ=255) gAACTGACGGTAACGATTCTGGCTAAACCATGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGc > 1:2210830/1‑69 (MQ=255) gAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGc > 1:1193420/1‑69 (MQ=255) gAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGc > 1:4829160/1‑69 (MQ=255) gAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGt < 1:2283205/71‑1 (MQ=255) cTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGc < 1:1960028/47‑1 (MQ=255) cTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGc < 1:3323221/47‑1 (MQ=255) tGACGGTAACGATTCTTGCTAAACCACGCCACGATGACGTTATCGc < 1:132216/46‑1 (MQ=39) tGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTAcgc > 1:2141466/1‑71 (MQ=255) | CCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTACGC > W3110S.gb/3521513‑3521641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |