| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,532,412 | T→C | 38.6% | M236T (ATG→ACG) | cpxA → | sensory histidine kinase in two‑component regulatory system with CpxR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,532,412 | 0 | T | C | 38.6% | 28.3 / 48.9 | 44 | M236T (ATG→ACG) | cpxA | sensory histidine kinase in two‑component regulatory system with CpxR |
| Reads supporting (aligned to +/- strand): ref base T (17/10); new base C (7/10); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CGGGGCCACAGGAATTCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGCAACT > W3110S.gb/3532343‑3532481 | cGGGGCCACAGGAATTCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCatgatg < 1:5000403/71‑1 (MQ=255) acaGGAATTCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACctct > 1:3887654/1‑70 (MQ=255) aGGAATTCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACctct > 1:175452/1‑68 (MQ=255) ggAATTCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTcag > 1:1919880/1‑70 (MQ=255) ttCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCAtgatga < 1:797441/58‑1 (MQ=255) ccTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTc > 1:829221/1‑71 (MQ=255) cTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCt > 1:233529/1‑71 (MQ=255) gCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCt > 1:4084551/1‑71 (MQ=255) gCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCt > 1:2593329/1‑71 (MQ=255) gCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTc < 1:1525810/71‑1 (MQ=255) cAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCt < 1:4563514/71‑1 (MQ=255) cAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCt < 1:3054382/71‑1 (MQ=255) gTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGat < 1:4000070/71‑1 (MQ=255) tGCCAGTTTTAACCAGATGGTAACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGata < 1:2068572/71‑1 (MQ=255) gCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTcag > 1:2890948/1‑51 (MQ=255) ccAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGat < 1:1084530/68‑1 (MQ=255) aGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGatat > 1:4748861/1‑68 (MQ=255) gTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtctct < 1:2236716/71‑1 (MQ=255) gTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATAtctct < 1:2484688/71‑1 (MQ=255) ttttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTca < 1:1356762/45‑1 (MQ=255) ttttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTca < 1:4912334/45‑1 (MQ=255) ttttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTca < 1:2077234/45‑1 (MQ=255) tttAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCa > 1:647695/1‑71 (MQ=255) tttAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCa > 1:3553624/1‑71 (MQ=255) ttAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGa > 1:1739026/1‑61 (MQ=255) ttAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCAc > 1:245513/1‑71 (MQ=255) ttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCAc > 1:2877921/1‑71 (MQ=255) ttAACCAGATGGTCACCGCGCTGGAGCGCATGACGACCTCTAAGCAGCGTCTGCTTTCTGATATCTCTCAc > 1:2616037/1‑71 (MQ=255) tAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCAc > 1:3024800/1‑70 (MQ=255) aaCCAGATGGTCACCGCGCTGGAGCGCATGATGAc > 1:3244905/1‑35 (MQ=255) tCACCGCGCTGTAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCAcc > 1:4208003/1‑71 (MQ=255) tCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCAcc > 1:4473343/1‑71 (MQ=255) tCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCAcc > 1:4126506/1‑71 (MQ=255) cACCGCGCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCAccc > 1:1605237/1‑71 (MQ=255) aCCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCAccc < 1:2272869/70‑1 (MQ=255) cgcgCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGc > 1:4858327/1‑71 (MQ=255) cgcgCTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGc > 1:860215/1‑71 (MQ=255) gcgcTGGAGCGCATGACGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCt < 1:1897007/71‑1 (MQ=255) gcgcTCGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCt < 1:1778872/71‑1 (MQ=255) gAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGGTGCGCACCCCGCTGAcgc < 1:2708634/70‑1 (MQ=255) gAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGAcgcg < 1:1149263/71‑1 (MQ=255) cATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCa > 1:3137048/1‑42 (MQ=255) cATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGc < 1:3949084/71‑1 (MQ=255) cATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGc < 1:3795452/71‑1 (MQ=255) cATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGc < 1:1706442/71‑1 (MQ=255) cATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGc < 1:1214548/71‑1 (MQ=255) tgacgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACg > 1:543085/1‑42 (MQ=255) tgacgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGAc < 1:3359150/61‑1 (MQ=255) tgacgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGCaa > 1:120981/1‑71 (MQ=255) gatgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGCAAc > 1:2047280/1‑71 (MQ=255) atgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGa > 1:4090670/1‑58 (MQ=255) atgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGa > 1:2683200/1‑58 (MQ=255) atgaCCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGCAACt > 1:1463370/1‑71 (MQ=255) | CGGGGCCACAGGAATTCCTTGCCGCAGGTGCCAGTTTTAACCAGATGGTCACCGCGCTGGAGCGCATGATGACCTCTCAGCAGCGTCTGCTTTCTGATATCTCTCACGAGCTGCGCACCCCGCTGACGCGTCTGCAACT > W3110S.gb/3532343‑3532481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |