| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,533,883 | T→C | 36.6% | intergenic (‑24/+125) | yiiM ← / ← kdgT | conserved hypothetical protein/2‑keto‑3‑deoxy‑D‑gluconate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,533,883 | 0 | T | C | 36.6% | 85.9 / 95.3 | 93 | intergenic (‑24/+125) | yiiM/kdgT | conserved hypothetical protein/2‑keto‑3‑deoxy‑D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base T (28/30); new base C (15/18); total (44/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTCAA > W3110S.gb/3533815‑3533953 | aTAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTcc > 1:1693142/1‑71 (MQ=255)aTAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTcc < 1:1194245/71‑1 (MQ=255)aTAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTcc > 1:1512979/1‑71 (MQ=255) tAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTccc < 1:1279623/71‑1 (MQ=255) gCCTGAATCTTGGCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCAt < 1:1927902/71‑1 (MQ=255) gCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCGTCTCCACGAGAAATTCCCCCAt < 1:744791/71‑1 (MQ=255) tGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCAt < 1:4698390/68‑1 (MQ=255) gAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGa > 1:4276226/1‑71 (MQ=255) aaTCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTa < 1:2790918/68‑1 (MQ=255) aaTCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGa < 1:3060628/70‑1 (MQ=255) aaTCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGAc > 1:4347357/1‑71 (MQ=255) cTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCa > 1:586986/1‑70 (MQ=255) gCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCAtatt > 1:1795656/1‑71 (MQ=255) gCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCata < 1:2216013/69‑1 (MQ=255) tgtATACGTCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTa > 1:3925134/1‑57 (MQ=255) tgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATatttattt < 1:2564705/71‑1 (MQ=255) tgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTa > 1:4053947/1‑57 (MQ=255) tataCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATatttattt < 1:940099/69‑1 (MQ=255) tataCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATAtttatttt < 1:4078231/70‑1 (MQ=255) tataCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATAtttatttt < 1:1270645/70‑1 (MQ=255) tataCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTACCCCATTAGACCATAtttatttt > 1:1992415/1‑70 (MQ=255) tataCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATT‑‑CCCATTAGACCATAtttatttt < 1:2336053/68‑1 (MQ=255) taCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTccc > 1:3069847/1‑48 (MQ=255) taCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATAtttattttat < 1:1131875/70‑1 (MQ=255) taCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATg > 1:4824213/1‑71 (MQ=255) taCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATg > 1:3913287/1‑71 (MQ=255) aCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCAtattta > 1:2071044/1‑63 (MQ=255) aCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGt > 1:767692/1‑71 (MQ=255) cATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt > 1:4682804/1‑70 (MQ=255) cATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt < 1:3278920/70‑1 (MQ=255) aTCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTa > 1:1861918/1‑50 (MQ=255) aTCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTa > 1:3359300/1‑50 (MQ=255) tCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTa < 1:2754657/49‑1 (MQ=255) tCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTa < 1:3314368/49‑1 (MQ=255) tCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAg < 1:1835205/71‑1 (MQ=255) aaCCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAg > 1:325496/1‑69 (MQ=255) aaCCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGc > 1:32391/1‑71 (MQ=255) aaCCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTa > 1:3175869/1‑47 (MQ=255) ccGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGt < 1:1130745/71‑1 (MQ=255) atatCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTg < 1:3535960/71‑1 (MQ=255) atatCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTg < 1:2788296/71‑1 (MQ=255) tatCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTg < 1:3363138/70‑1 (MQ=255) tCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGttt < 1:778332/71‑1 (MQ=255) tCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGttt < 1:2043733/71‑1 (MQ=255) tCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTa > 1:1629274/1‑38 (MQ=255) tCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTa > 1:2728748/1‑38 (MQ=255) tCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGtt < 1:305869/70‑1 (MQ=255) tCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGtt < 1:2058122/70‑1 (MQ=255) gCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTc > 1:1132100/1‑71 (MQ=255) gCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTc > 1:3961633/1‑71 (MQ=255) gCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTc > 1:929133/1‑71 (MQ=255) gCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTc < 1:4097292/71‑1 (MQ=255) gCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTc < 1:4351751/71‑1 (MQ=255) cATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTcg > 1:5100962/1‑71 (MQ=255) tCTGTTCCCTCTCAACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt < 1:5029877/52‑1 (MQ=255) cTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATAtttatttt < 1:4635763/47‑1 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCAtatttatt > 1:2919854/1‑44 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATAtttatttta > 1:1391407/1‑47 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATAtttatttta > 1:2705876/1‑47 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATAtttatttta > 1:1397439/1‑47 (MQ=255) gTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt < 1:4110133/49‑1 (MQ=255) ttCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCgg < 1:713618/68‑1 (MQ=255) ttCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGt < 1:809350/69‑1 (MQ=255) ttCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTcgcg < 1:1363424/67‑1 (MQ=255) ttCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTcgcg < 1:4655275/67‑1 (MQ=255) ttCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTaa < 1:337683/71‑1 (MQ=255) cccTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAg > 1:2005532/1‑70 (MQ=255) cccTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTaa < 1:331549/69‑1 (MQ=255) cccTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAg > 1:1128570/1‑70 (MQ=255) cccTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAg < 1:606816/70‑1 (MQ=255) ctcCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAg > 1:2083745/1‑45 (MQ=255) tcCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATc > 1:1093383/1‑69 (MQ=255) tcCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATc < 1:3095668/69‑1 (MQ=255) tcCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATc > 1:3332224/1‑69 (MQ=255) ccACGAGAAATTCCCCCATTAGACCATatttattt < 1:2935680/35‑1 (MQ=255) cACGAGAAATTTCCCCATTAGACCATAtttatttt > 1:1875900/1‑35 (MQ=255) cACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcagg > 1:4727637/1‑71 (MQ=255) cACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGt > 1:3475161/1‑51 (MQ=255) cACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGt > 1:4708286/1‑51 (MQ=255) cACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGt > 1:271139/1‑51 (MQ=255) aCGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGtt < 1:4322498/51‑1 (MQ=255) cGAGAAATTTCCCCATTAGACCATAtttattttat < 1:519885/35‑1 (MQ=255) cGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCa > 1:2710317/1‑39 (MQ=255) gagaAATTTCCCCATTAGACCATATTTATTTTATGTCAgg > 1:941664/1‑40 (MQ=255) gagaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGt < 1:3301835/43‑1 (MQ=255) gagaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggcag < 1:4135869/71‑1 (MQ=255) gaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGtttt < 1:76700/49‑1 (MQ=255) gaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGtttt < 1:1482613/49‑1 (MQ=255) gaAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGa < 1:3597748/71‑1 (MQ=255) aaaTTTCCCCATTAGACCATATTTATTTTATGTCa > 1:2404544/1‑35 (MQ=255) aaaTTTCCCCATTAGACCATATTTATTTTATGTCa > 1:1323318/1‑35 (MQ=255) aTTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGt < 1:2923171/56‑1 (MQ=255) aTTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggca < 1:3083298/65‑1 (MQ=255) tttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTc < 1:1674921/71‑1 (MQ=255) tCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTCaa > 1:4178394/1‑71 (MQ=255) | ATAAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTCAA > W3110S.gb/3533815‑3533953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |