| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,564,985 | T→C | 29.8% | F21L (TTT→CTT) | yihS → | predicted glucosamine isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,564,985 | 0 | T | C | 29.8% | 63.5 / 37.4 | 47 | F21L (TTT→CTT) | yihS | predicted glucosamine isomerase |
| Reads supporting (aligned to +/- strand): ref base T (15/18); new base C (9/5); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAAAGGAATGAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATAAAGGGCAAATCA > W3110S.gb/3564917‑3565051 | tAAAAGGAATGAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCttt < 1:4944489/71‑1 (MQ=255)tAAAAGGAATGAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGAACGCATCttt < 1:429655/71‑1 (MQ=255) aaaaGGAATGAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCctt > 1:1589011/1‑70 (MQ=255) aaaaGGAATGAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCctt > 1:4695779/1‑70 (MQ=255) aGGAATGAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGAtt < 1:2253590/71‑1 (MQ=255) ggAATGAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGAttt < 1:2982501/71‑1 (MQ=255) gAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGt > 1:1979839/1‑70 (MQ=255) aaaTGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTa < 1:2834635/70‑1 (MQ=255) aaaTGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTa > 1:4898519/1‑70 (MQ=255) aTGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTaaa > 1:3730606/1‑70 (MQ=255) tGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTaaaaa > 1:3738647/1‑71 (MQ=255) tGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTaaaaa < 1:3400643/71‑1 (MQ=255) gTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAAtt > 1:3520366/1‑71 (MQ=255) tttAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTc < 1:5102347/71‑1 (MQ=255) acacCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCg < 1:3348542/69‑1 (MQ=255) acacCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGt < 1:4186897/70‑1 (MQ=255) acacCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGt < 1:4819518/70‑1 (MQ=255) cccTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCg < 1:3084037/66‑1 (MQ=255) ccTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCt < 1:1681279/44‑1 (MQ=255) cTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAAt > 1:4126793/1‑60 (MQ=255) cTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTa > 1:850788/1‑66 (MQ=255) cTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTa > 1:182346/1‑66 (MQ=255) cTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGcc > 1:2747598/1‑71 (MQ=255) cTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGcc > 1:2902009/1‑71 (MQ=255) cTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGTAGTGcc < 1:2667358/71‑1 (MQ=255) tAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGTAg > 1:597141/1‑66 (MQ=255) aGCCACAACCGTTGGCTGGAACAGGAAACCGCCCGCATCTTTGATTTTGGTAAAAATTCCGTAGTgac > 1:4527379/1‑66 (MQ=255) acaACCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGt > 1:2428076/1‑71 (MQ=255) acaACCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGt > 1:3086710/1‑71 (MQ=255) caACCGTTGGCTGCAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGtt > 1:1938676/1‑71 (MQ=255) aCCGTTGGCTGGAACAGTAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGtttt > 1:324013/1‑71 (MQ=255) aCCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGtttt < 1:3218888/71‑1 (MQ=255) aCCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGtttt > 1:3583797/1‑71 (MQ=255) aCCGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGtttt > 1:3665382/1‑71 (MQ=255) ccGTTGGCTGGAACAGGAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTg > 1:4579502/1‑71 (MQ=255) tGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGt > 1:1819521/1‑69 (MQ=255) aaCAGGAAACCGACCGCATCTTTGATTTTGGTaaaaa > 1:99105/1‑37 (MQ=255) aCAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCg < 1:4852940/49‑1 (MQ=255) ggAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATa < 1:4782204/71‑1 (MQ=255) ggAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATa < 1:2292615/71‑1 (MQ=255) ggAAACCGACCGCATCCTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATa < 1:2291360/71‑1 (MQ=255) gAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATa < 1:3163796/70‑1 (MQ=255) ccgaccgCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAAt < 1:4911379/65‑1 (MQ=255) ccgaccgCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAAt < 1:450566/65‑1 (MQ=255) ccgCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATaa < 1:678707/63‑1 (MQ=255) ccgCATCTTTGATTTTGGTAAAAATTACGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATaa < 1:1126025/63‑1 (MQ=255) gCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATAAAg > 1:2561613/1‑63 (MQ=255) cATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATAAAGGGCAAATCa < 1:3193835/71‑1 (MQ=255) cATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATAAAGGGCAAATCa < 1:667402/71‑1 (MQ=255) cATCCTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATAAAGGGCAAATc > 1:2391001/1‑70 (MQ=255) aTCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATAAAGGTCAAATCa < 1:2067105/70‑1 (MQ=255) aTCCTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATAAAg > 1:705403/1‑61 (MQ=255) tttGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTa > 1:170701/1‑48 (MQ=255) | TAAAAGGAATGAAATGGTTTAACACCCTAAGCCACAACCGTTGGCTGGAACAGGAAACCGACCGCATCTTTGATTTTGGTAAAAATTCCGTAGTGCCGACTGGTTTTGGCTGGTTAGGCAATAAAGGGCAAATCA > W3110S.gb/3564917‑3565051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |