| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,584,576 | G→A | 19.0% | P21S (CCA→TCA) | hemN ← | coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,584,576 | 0 | G | A | 19.0% | 81.9 / 13.4 | 37 | P21S (CCA→TCA) | hemN | coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base A (4/3); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCTCACTCCT > W3110S.gb/3584511‑3584646 | tAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCgg < 1:5091943/71‑1 (MQ=255) aaaaCGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCAGGaa > 1:1447373/1‑71 (MQ=255) ccTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTta < 1:4118479/71‑1 (MQ=255) ttCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCgg < 1:2294190/59‑1 (MQ=255) cGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATAttt < 1:1504330/70‑1 (MQ=255) cGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTc > 1:1135847/1‑71 (MQ=255) cGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTc > 1:2546811/1‑71 (MQ=255) tcttctGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGTCCCGGAATAGTTATAtt < 1:5015730/61‑1 (MQ=255) tcttctGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCgg < 1:1139399/49‑1 (MQ=255) ctGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAggg < 1:3838584/69‑1 (MQ=255) ctGAAAACTACAGCGCGGTCGGGTACGAGGTGTATCGTGGcac > 1:4274991/1‑41 (MQ=255) gAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGc > 1:4618418/1‑68 (MQ=255) aaaaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCa < 1:2111899/69‑1 (MQ=255) aaaaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGa < 1:1180121/71‑1 (MQ=255) aaCTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATc > 1:2494233/1‑71 (MQ=255) cTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAgg > 1:1089639/1‑61 (MQ=255) cTCCAGCGCGGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTCTTGATCAGGGCCAGATcc > 1:4217076/1‑70 (MQ=255) aGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTc < 1:1479891/71‑1 (MQ=255) gcgcGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGa > 1:2930030/1‑51 (MQ=255) gcgcGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGa > 1:4333759/1‑51 (MQ=255) cgGTCGGGTACGAGGTGTATCGTGACCCGGAATAGTTATATTTc > 1:314883/1‑44 (MQ=255) tggggTACGAGGCGTATCGTGGCCCGGAATAGTTATATTTCTGGa < 1:4817488/43‑1 (MQ=38) tCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGa < 1:1149233/45‑1 (MQ=255) tCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGa < 1:4020875/45‑1 (MQ=255) tCGGGTACGAGGCGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTc < 1:48250/64‑1 (MQ=255) gggTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTg > 1:3181044/1‑71 (MQ=255) cGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGAt < 1:1223484/39‑1 (MQ=255) cGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTa > 1:2694224/1‑68 (MQ=255) cGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAg < 1:3193887/71‑1 (MQ=255) cGAGGTGTATCGTGACCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAg < 1:305623/71‑1 (MQ=255) ggTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACa > 1:3905686/1‑71 (MQ=255) tgtATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATg > 1:2798011/1‑71 (MQ=255) tgtATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATg < 1:1665028/71‑1 (MQ=255) tATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCt > 1:5007539/1‑71 (MQ=255) tATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCt < 1:2454862/71‑1 (MQ=255) gTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCa > 1:3353493/1‑43 (MQ=255) gTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCa > 1:4862915/1‑43 (MQ=255) gCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCTCACTCCt < 1:3029314/71‑1 (MQ=255) | TAAAAACGCCTGTTCGCCGAAGTCTTCTGAAAACTCCAGCGCGGTCGGGTACGAGGTGTATCGTGGCCCGGAATAGTTATATTTCTGGATCAGGGCCAGATCCCAGTCGATTTGCTGTACAGACATGCTCACTCCT > W3110S.gb/3584511‑3584646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |