| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,609,702 | C→T | 23.8% | E151E (GAG→GAA) | fre ← | flavin reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,609,702 | 0 | C | T | 23.8% | 80.6 / 22.6 | 42 | E151E (GAG→GAA) | fre | flavin reductase |
| Reads supporting (aligned to +/- strand): ref base C (17/15); new base T (6/4); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
AGCCCGCTTCCGGTTGTTCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGATATCACGGTTTGGGT > W3110S.gb/3609632‑3609769 | aGCCCGCTTCCGGTTGTTCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGc < 1:4280318/71‑1 (MQ=255) ccGCTTCCGGTTGTTCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCtag > 1:4248671/1‑69 (MQ=255) ccGCTTCCGGTTGTTCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCtag > 1:4243039/1‑69 (MQ=255) ccGGTTGTTCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCaga < 1:1781378/69‑1 (MQ=255) ccGGTTGTTCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCa > 1:444065/1‑67 (MQ=255) gttCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATa < 1:4815300/70‑1 (MQ=255) gttCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGACTCAAGCTCGCAGAGATCATa > 1:256542/1‑70 (MQ=255) aaCCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGa < 1:2085256/70‑1 (MQ=255) aaCCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGAt < 1:2434960/71‑1 (MQ=255) aaCCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGACTCAAGCTCGCAGAGATCATACAg < 1:4098493/69‑1 (MQ=255) ccaccGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGAtgc < 1:1420348/71‑1 (MQ=255) accGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGAtgctg > 1:3597781/1‑71 (MQ=255) caccacTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCtt < 1:2094371/71‑1 (MQ=255) ccacTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCa < 1:2653734/46‑1 (MQ=255) gCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCAcgcccg > 1:3424127/1‑71 (MQ=255) cAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAg > 1:3812327/1‑71 (MQ=255) ggATGCTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTa < 1:2358862/71‑1 (MQ=255) ggATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATa > 1:276200/1‑41 (MQ=255) ggATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATa > 1:1252822/1‑41 (MQ=255) ggATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTc > 1:2566968/1‑56 (MQ=255) ggATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCa > 1:1715897/1‑57 (MQ=255) aTGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGctct > 1:611940/1‑52 (MQ=255) aTGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaaa < 1:2581742/71‑1 (MQ=255) tGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAAt < 1:2279388/71‑1 (MQ=255) cTTCAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATgg > 1:3348283/1‑71 (MQ=255) cTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCAcgcccgc > 1:2179151/1‑59 (MQ=255) cAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGa > 1:267433/1‑71 (MQ=255) cAACGAAAGCGCCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGa > 1:1000485/1‑71 (MQ=255) cAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGt < 1:4498261/69‑1 (MQ=255) aaCGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGat < 1:173465/71‑1 (MQ=255) aCGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCa > 1:1440772/1‑58 (MQ=255) aCGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGATGCTCTTc > 1:3164891/1‑46 (MQ=255) gAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGatat > 1:1517527/1‑70 (MQ=255) gcgcCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGt < 1:3914594/61‑1 (MQ=255) gcCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAAt > 1:4571728/1‑56 (MQ=255) gcCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAAt > 1:478049/1‑56 (MQ=255) gcCTCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAAt > 1:790163/1‑56 (MQ=255) tCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa < 1:3610260/51‑1 (MQ=255) tCAAGTTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTaa < 1:1748529/51‑1 (MQ=255) aaGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATg > 1:2665416/1‑52 (MQ=255) aaGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGATATCACGGTTTGGGt < 1:3589373/71‑1 (MQ=255) aaGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGATATCACGGTTTGGGt < 1:2562670/71‑1 (MQ=255) aaGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGATATCACGGTTTGGGt < 1:4737813/71‑1 (MQ=255) | AGCCCGCTTCCGGTTGTTCAACCACCGGCACCACTTGCAGACCAGGATGCTTCAACGAAAGCGCCTCAAGCTCGCAGAGATCATACAGATGCTGCTCTTCACGCCCGCCCCAGTAAATGGTGATATCACGGTTTGGGT > W3110S.gb/3609632‑3609769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |