| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 267,502 | G→T | 32.7% | P228H (CCT→CAT) | ykfA ← | predicted GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 267,502 | 0 | G | T | 32.7% | 106.7 / 37.7 | 58 | P228H (CCT→CAT) | ykfA | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (20/19); new base T (7/12); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
GGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCA > W3110S.gb/267438‑267568 | ggCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:2248932/71‑1 (MQ=255)ggCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATg > 1:4955495/1‑69 (MQ=255) cGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc < 1:2715526/71‑1 (MQ=255) gACGGTTTCCCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAg > 1:668444/1‑66 (MQ=255) gACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAATGGAAGGATGGAGTTGc > 1:891279/1‑70 (MQ=255) gACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc > 1:3207248/1‑70 (MQ=255) gACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc > 1:3977844/1‑70 (MQ=255) gACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc < 1:4497244/70‑1 (MQ=255) aCGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa < 1:4321338/71‑1 (MQ=255) aCGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:1610013/1‑71 (MQ=255) aCGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:3871045/1‑71 (MQ=255) gTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATgg < 1:3997252/60‑1 (MQ=255) gTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATgg < 1:565995/60‑1 (MQ=255) gTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGacac < 1:95475/71‑1 (MQ=255) gTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGaca < 1:720735/70‑1 (MQ=255) tttCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGtt < 1:3204326/63‑1 (MQ=255) tttCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGtt < 1:2627451/63‑1 (MQ=255) ttCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAaggaagga > 1:4656710/1‑55 (MQ=255) ttCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAg > 1:2305251/1‑60 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:519822/1‑65 (MQ=255) cAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCTAAAGGAAGGATGGAGTTGCGACACCACCgg > 1:4007472/1‑70 (MQ=255) aaaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGa > 1:3261391/1‑53 (MQ=255) aaTCGCTACGAGCCTGTTGCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGtt < 1:312514/54‑1 (MQ=255) aTCGCTACGAGCCTGTTCCCGGACTTCCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCtgg > 1:2782622/1‑71 (MQ=255) aTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCtgg > 1:1835898/1‑71 (MQ=255) aTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtgg > 1:1907354/1‑71 (MQ=255) tCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc > 1:606428/1‑54 (MQ=255) tCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc > 1:3784186/1‑54 (MQ=255) cTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGc < 1:4484120/71‑1 (MQ=255) tACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGCCACCACCGGGCTGGTGGc > 1:3962296/1‑70 (MQ=255) tACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGc > 1:4566097/1‑70 (MQ=255) aCGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt > 1:4923040/1‑71 (MQ=255) aCGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt > 1:3503090/1‑71 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACCCCACCGGGCTGGTGGCCt > 1:164839/1‑70 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGcc < 1:1260165/69‑1 (MQ=255) ccTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGt < 1:1010747/71‑1 (MQ=255) gTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCAc < 1:2954201/66‑1 (MQ=255) gTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCAc < 1:1038634/66‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGa < 1:3866301/35‑1 (MQ=255) ccGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg < 1:1759901/70‑1 (MQ=255) tgACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCAc > 1:4789710/2‑71 (MQ=255) ggACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCg > 1:2314956/1‑45 (MQ=255) aCTACCGTTGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg < 1:2806602/48‑1 (MQ=255) cTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACtt < 1:4255877/70‑1 (MQ=255) gtcgtGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCt < 1:703408/41‑1 (MQ=255) gtcgtGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatcat < 1:2790506/71‑1 (MQ=255) tcgtGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTg < 1:136857/54‑1 (MQ=255) tcgtGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatcatc < 1:1864672/71‑1 (MQ=255) tcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg < 1:4413569/41‑1 (MQ=255) tcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg < 1:4151192/41‑1 (MQ=255) tcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg < 1:857627/41‑1 (MQ=255) tcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg < 1:1359003/41‑1 (MQ=255) tcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatcat < 1:2043141/70‑1 (MQ=255) gtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCg < 1:1594507/71‑1 (MQ=255) cGAAAGGAATGATGGAGTTGCGACACCCCCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTc > 1:45368/1‑71 (MQ=255) gAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatc < 1:2700178/62‑1 (MQ=255) aaaGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCgg > 1:2019602/1‑71 (MQ=255) aaaGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCgg > 1:4367334/1‑71 (MQ=255) aaaGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCgg > 1:3079119/1‑71 (MQ=255) aaggaaggATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGc < 1:2183750/71‑1 (MQ=255) aggaaggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTc > 1:1380622/1‑40 (MQ=255) ggaatgaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCa < 1:1148116/71‑1 (MQ=255) ggaatgaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCa < 1:935399/71‑1 (MQ=255) | GGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCA > W3110S.gb/267438‑267568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |