| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,639,730 | T→C | 35.7% | K222R (AAA→AGA) | xerC ← | site‑specific tyrosine recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,639,730 | 0 | T | C | 35.7% | 66.1 / 73.4 | 70 | K222R (AAA→AGA) | xerC | site‑specific tyrosine recombinase |
| Reads supporting (aligned to +/- strand): ref base T (17/28); new base C (11/14); total (28/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
AGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTA > W3110S.gb/3639662‑3639795 | aGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGtctc < 1:862325/71‑1 (MQ=255) tGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTg > 1:1824898/1‑71 (MQ=255) gACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTg > 1:3446331/1‑70 (MQ=255) aCGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGca > 1:860678/1‑71 (MQ=255) aCGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGca > 1:4607190/1‑71 (MQ=255) aCGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGca > 1:3940503/1‑71 (MQ=255) atatATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTa > 1:2642485/4‑71 (MQ=255) aTTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt > 1:4501243/1‑61 (MQ=255) tttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAc > 1:4276813/1‑71 (MQ=255) tttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAc > 1:4282446/1‑71 (MQ=255) cGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGtctc < 1:4379241/55‑1 (MQ=255) cGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgcg < 1:4089609/70‑1 (MQ=255) aTGAACGTTATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGgaga < 1:3170685/71‑1 (MQ=255) aTGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACAt > 1:4931163/1‑59 (MQ=255) aTGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGgaga < 1:3056689/71‑1 (MQ=255) aTGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGgaga < 1:2469410/71‑1 (MQ=255) aaCGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt > 1:1240561/1‑48 (MQ=255) aaCGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt > 1:535208/1‑48 (MQ=255) aaCGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt > 1:1842269/1‑48 (MQ=255) aaCGTGATTATTCAGCCCTTGTTTTATGCCACATTCGGCAAAGCGTTTCTGCACATTAcg > 1:2918064/1‑60 (MQ=255) cGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGc > 1:3205705/1‑71 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACAt < 1:2314979/53‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACAt < 1:4561696/53‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACAt < 1:4039090/53‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCt < 1:603982/71‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCt < 1:4061325/71‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGAAAAGCGTCTCTGCACATTACGCGCGGAGATACGCt < 1:1459016/71‑1 (MQ=255) tGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCtt < 1:4118472/71‑1 (MQ=255) gATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGcaca > 1:560437/1‑50 (MQ=255) attattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACAt < 1:1654517/50‑1 (MQ=255) attattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACAt < 1:3943326/50‑1 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa < 1:2307490/51‑1 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa < 1:4278263/51‑1 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa < 1:4993854/51‑1 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa < 1:3502550/51‑1 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa < 1:1242189/51‑1 (MQ=255) attCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTctct > 1:3131971/1‑41 (MQ=255) attCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTctct > 1:720799/1‑41 (MQ=255) ttCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGAt < 1:1203060/59‑1 (MQ=255) ttCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGAt < 1:4325503/59‑1 (MQ=255) cTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGga < 1:525249/49‑1 (MQ=255) cTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGgaga < 1:4270061/51‑1 (MQ=255) cTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:3567515/1‑70 (MQ=255) tGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGgaga > 1:3932372/1‑49 (MQ=255) tGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGAGCGgaga > 1:1774728/1‑49 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAg < 1:4397031/70‑1 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGga > 1:913376/1‑46 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTc > 1:1749/1‑65 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTc > 1:591907/1‑65 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaa < 1:3207965/70‑1 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaa < 1:1287199/70‑1 (MQ=255) tATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaaa < 1:273603/70‑1 (MQ=255) tATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaaa < 1:2603067/70‑1 (MQ=255) tATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaaa < 1:50710/70‑1 (MQ=255) tATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaaa < 1:141895/70‑1 (MQ=255) aTGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAg > 1:3692715/1‑71 (MQ=255) ccccATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAgcg < 1:527303/70‑1 (MQ=255) ccccATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAgcg < 1:1445720/70‑1 (MQ=255) cccATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGcgcg < 1:3461454/71‑1 (MQ=255) cATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:2096923/1‑71 (MQ=255) cATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:2406119/1‑71 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGAt < 1:1860534/36‑1 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGAt < 1:237147/36‑1 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGAt < 1:2504801/36‑1 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCg < 1:3267093/53‑1 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAgcgc < 1:4860807/66‑1 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:1557978/1‑71 (MQ=255) ttCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATAc < 1:2015541/38‑1 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCg < 1:3069417/51‑1 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:2599512/1‑69 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:348638/1‑69 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt > 1:3593757/1‑71 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt > 1:1273151/1‑71 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt > 1:2493667/1‑71 (MQ=255) cGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGc > 1:2221238/1‑38 (MQ=255) cGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCgtcgtc < 1:5111296/70‑1 (MQ=255) ggCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt < 1:1346177/70‑1 (MQ=255) aGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTa < 1:2433105/71‑1 (MQ=255) tttCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTc < 1:3000389/63‑1 (MQ=255) ttCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc < 1:4550742/56‑1 (MQ=255) | AGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTA > W3110S.gb/3639662‑3639795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |