| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,657,262 | C→T | 25.9% | E273E (GAG→GAA) | wzyE ← | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,657,262 | 0 | C | T | 25.9% | 156.7 / 56.1 | 86 | E273E (GAG→GAA) | wzyE | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Reads supporting (aligned to +/- strand): ref base C (35/28); new base T (12/10); total (48/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCACATT > W3110S.gb/3657195‑3657330 | cgcgGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGAtcctcc > 1:4237296/1‑71 (MQ=255) gcgGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTccc < 1:4963938/71‑1 (MQ=255) cAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGc < 1:326196/71‑1 (MQ=255) aaTTGGAGCCAGGCCCTGTAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGc > 1:819244/1‑70 (MQ=255) aaTTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGc > 1:559137/1‑70 (MQ=255) aaTTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGc > 1:700431/1‑70 (MQ=255) aTTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCga < 1:1448995/71‑1 (MQ=255) ttGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCga < 1:3230834/70‑1 (MQ=255) ggAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCgag > 1:3630011/1‑69 (MQ=255) gAGCCAGGCCCTGGAAGTCGTTGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGaa < 1:4947025/70‑1 (MQ=255) gAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGaa < 1:3436229/70‑1 (MQ=255) gAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGaa < 1:4358206/70‑1 (MQ=255) gCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGgt < 1:2789630/71‑1 (MQ=255) ccAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGgtg < 1:2410135/71‑1 (MQ=255) gCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCgag < 1:3483914/61‑1 (MQ=255) cccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATtct > 1:3275543/1‑49 (MQ=255) cccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATtct > 1:4930722/1‑49 (MQ=255) cccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgcg < 1:5134665/71‑1 (MQ=255) ccTTGAAGTCGATGTTGTCGTAGTTCTGCAACATCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc > 1:2229556/1‑69 (MQ=255) ccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTcgc > 1:4116187/1‑69 (MQ=255) ccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc > 1:4820346/1‑69 (MQ=255) ccTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACAGCGAGAAGGTGTcgc > 1:1196049/1‑69 (MQ=255) gAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGt < 1:1570024/69‑1 (MQ=255) aaGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCa > 1:505399/1‑47 (MQ=255) gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACgg > 1:205035/1‑48 (MQ=255) gTCGATGTTGTCGTAGCTCTGCAACAGCAACGCCAGATTCTCCCACgg > 1:2459675/1‑48 (MQ=255) cGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:4840137/1‑68 (MQ=255) gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:3969625/1‑69 (MQ=255) gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:520406/1‑69 (MQ=255) tgttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATagag > 1:2713406/1‑70 (MQ=255) gttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAgaga > 1:2431832/1‑70 (MQ=255) gttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAgaga > 1:4656409/1‑70 (MQ=255) ttgtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGaa > 1:751446/1‑47 (MQ=255) ttgtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAg < 1:3925429/48‑1 (MQ=255) ttgtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGAt > 1:349385/1‑64 (MQ=255) ttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaa < 1:4060153/70‑1 (MQ=255) ttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaa < 1:857133/70‑1 (MQ=255) gtCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg > 1:5842/1‑71 (MQ=255) gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAg < 1:407108/46‑1 (MQ=255) gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAg < 1:1533089/46‑1 (MQ=255) gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg > 1:1642710/1‑71 (MQ=255) gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg > 1:4491743/1‑71 (MQ=255) tCGTAGCTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg < 1:971212/70‑1 (MQ=255) gTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg > 1:1455125/1‑53 (MQ=255) gTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg > 1:1942499/1‑53 (MQ=255) tAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg < 1:2718357/52‑1 (MQ=255) tAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg < 1:1585836/52‑1 (MQ=255) gTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAg > 1:215276/1‑40 (MQ=255) ttCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc < 1:2399736/46‑1 (MQ=255) cTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:594085/1‑71 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaa > 1:3589396/1‑59 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:1133549/1‑71 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:594067/1‑71 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGAGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:3727403/1‑71 (MQ=255) cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg < 1:4854949/68‑1 (MQ=255) cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg < 1:2511174/68‑1 (MQ=255) cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACATATAGAACGCt < 1:4777252/70‑1 (MQ=255) aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:3631769/1‑50 (MQ=255) aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:2471381/1‑50 (MQ=255) aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:453331/1‑50 (MQ=255) aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCtt < 1:1294256/70‑1 (MQ=255) aaCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTc < 1:4832375/71‑1 (MQ=255) aCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg < 1:2535641/66‑1 (MQ=255) aCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCa < 1:2080751/71‑1 (MQ=255) cAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCAt > 1:1524116/1‑71 (MQ=255) cAGAAACGCCAGATTATCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTc > 1:1970497/1‑69 (MQ=255) aGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGa > 1:3385674/1‑39 (MQ=255) aGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATc > 1:2606186/1‑71 (MQ=255) gCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:3541634/1‑44 (MQ=255) gCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:2038536/1‑44 (MQ=255) gCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcg > 1:3381764/1‑71 (MQ=255) gCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATc > 1:2168600/1‑70 (MQ=255) aCGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgcag > 1:4806466/1‑69 (MQ=255) gCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgccctc < 1:4529637/71‑4 (MQ=255) gCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCt > 1:3890424/1‑71 (MQ=255) ccAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTc < 1:4431622/71‑1 (MQ=255) ccAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTc < 1:2797209/71‑1 (MQ=255) ccAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:3286395/1‑38 (MQ=255) ccAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg < 1:2232831/48‑1 (MQ=255) cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgc > 1:1580324/1‑65 (MQ=255) cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTca > 1:1760305/1‑71 (MQ=255) cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTca < 1:1484149/71‑1 (MQ=255) cAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTc < 1:2256105/70‑1 (MQ=255) aGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTca > 1:256936/1‑70 (MQ=255) gATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTcaca < 1:3865546/71‑1 (MQ=255) gATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTcaca < 1:2396312/71‑1 (MQ=255) aTTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTTTAGAACGCTTCATCGCCGCTcaca < 1:1929552/70‑1 (MQ=255) aTTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAg < 1:2754788/49‑1 (MQ=255) ttCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCAcatt > 1:4259273/1‑71 (MQ=255) tctcCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgc < 1:3446915/60‑1 (MQ=255) tctcCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgc < 1:3339340/60‑1 (MQ=255) | CGCGGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCACATT > W3110S.gb/3657195‑3657330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |