| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,659,714 | G→A | 5.6% | T230T (ACC→ACT) | wzxE ← | O‑antigen translocase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,659,714 | 0 | G | A | 5.6% | 340.5 / 7.3 | 107 | T230T (ACC→ACT) | wzxE | O‑antigen translocase |
| Reads supporting (aligned to +/- strand): ref base G (61/39); new base A (3/3); total (64/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GATCCCCACCTCATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATCCCAGCTGG > W3110S.gb/3659645‑3659784 | gATCCCCACCTCATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAgg > 1:1634197/1‑71 (MQ=255) tCCCCACCTCATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGt > 1:3921757/1‑70 (MQ=255) tCCCCACCTCATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGt > 1:4420363/1‑70 (MQ=255) ccccACCTCATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCa > 1:1888877/1‑71 (MQ=255) cccACCTCATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCAc > 1:3548599/1‑71 (MQ=255) aCCTCATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCAcc > 1:410378/1‑69 (MQ=255) cATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCAc > 1:2711535/1‑64 (MQ=255) aTCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACTGGCAAGGTCACCGACGt < 1:303414/69‑1 (MQ=255) aTCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTaa > 1:1053396/1‑71 (MQ=255) ccAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCAc > 1:1008390/1‑60 (MQ=255) ccAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATc > 1:318802/1‑70 (MQ=255) ccAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATc < 1:2045936/70‑1 (MQ=255) gCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAg < 1:2302820/52‑1 (MQ=255) gCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCa < 1:298341/56‑1 (MQ=255) gCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCAcc < 1:4315938/58‑1 (MQ=255) cTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGc > 1:2750901/1‑71 (MQ=255) cTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGc > 1:2767717/1‑71 (MQ=255) cTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGc > 1:1163677/1‑71 (MQ=255) tataCTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGa > 1:4487438/1‑58 (MQ=255) ataCTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCa > 1:4924474/1‑53 (MQ=255) ataCTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCg < 1:2379778/56‑1 (MQ=255) aCTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCaa > 1:3813129/1‑64 (MQ=255) aCTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCaa > 1:3757039/1‑64 (MQ=255) cTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAg > 1:4969187/1‑46 (MQ=255) cTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCaa > 1:4125026/1‑63 (MQ=255) cTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCaa > 1:3446482/1‑63 (MQ=255) cTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCAt > 1:3717925/1‑69 (MQ=255) cTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCAt > 1:424082/1‑69 (MQ=255) ccgccAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTa < 1:3489802/71‑1 (MQ=255) agcagTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGcc > 1:1218311/1‑57 (MQ=255) agcagTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGcc > 1:1212396/1‑57 (MQ=255) agcagTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGcc > 1:2102130/1‑57 (MQ=255) agcagTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGcc > 1:1431184/1‑57 (MQ=255) agcagTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGcc > 1:2525335/1‑57 (MQ=255) cagTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGc > 1:2204303/1‑71 (MQ=255) gTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTc < 1:3279203/71‑1 (MQ=255) tttACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTaa < 1:1025414/62‑1 (MQ=255) tACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCaa < 1:3703205/70‑1 (MQ=255) tACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAAc > 1:77803/1‑71 (MQ=255) tACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAAc > 1:1725156/1‑71 (MQ=255) tACGCATCATGATGTAAGCAACAGGCAAAGTCACCGACGTAATCAACGCCATGAGCGTAAAt > 1:5084130/1‑62 (MQ=255) cGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActg < 1:3792654/71‑1 (MQ=255) cGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActg < 1:1027341/71‑1 (MQ=255) tcatGATTTAAGCAACAGGCAACGTCACCGACGTAATc < 1:432335/38‑1 (MQ=37) tcatGATGTAAGCAACAGGCAAGGTCACCGACGTAATc < 1:278327/38‑1 (MQ=255) tcatGATGTAAGCAACAGGCAAGGTCACCGACGTAATc < 1:1829918/38‑1 (MQ=255) tcatGATGTAAGCAACAGGCAAGGTCACCGACGTAATc < 1:2077934/38‑1 (MQ=255) tcatGATGTAAGCAACAGGCAAGGTCACCGACGTAATc < 1:2132126/38‑1 (MQ=255) tcatGATGTAAGCAACAGGCAAGGTCACCGACGTAATc < 1:4999962/38‑1 (MQ=255) tcatGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTc < 1:646967/62‑1 (MQ=255) tcatGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTc < 1:3962805/62‑1 (MQ=255) tcatGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTc < 1:1587871/62‑1 (MQ=255) tcatGATGTAAGCAACAGGCAAAGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActgccct < 1:3600321/71‑1 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCaa > 1:3272801/1‑38 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCaa > 1:2115656/1‑38 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCaa > 1:4809271/1‑38 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGc > 1:815734/1‑41 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGc > 1:4844654/1‑41 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActgccctgc > 1:1784469/1‑71 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActgccctgc > 1:4325649/1‑71 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActgccctgc > 1:699178/1‑71 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActgccctgc > 1:425453/1‑71 (MQ=255) atgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAAct > 1:2675058/1‑64 (MQ=255) atgatgTAAGCAACAGGCAAAGTCACCGACGTAATCaa > 1:1423824/1‑38 (MQ=255) atgatgTAAGCAACAGGCAAAGTCACCGACGTAATCaa > 1:2531784/1‑38 (MQ=255) tgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCTTaa < 1:4702663/51‑1 (MQ=255) tgatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTaa < 1:3594047/51‑1 (MQ=255) gatgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAAc > 1:1075601/1‑61 (MQ=255) atgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAAt > 1:861416/1‑51 (MQ=255) atgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAAt > 1:1161467/1‑51 (MQ=255) atgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAAt > 1:1354366/1‑51 (MQ=255) atgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAAt > 1:3113472/1‑51 (MQ=255) atgTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAAt > 1:2702432/1‑51 (MQ=255) gTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTaa < 1:1585319/47‑1 (MQ=255) gTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTaa < 1:1685071/47‑1 (MQ=255) gTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTaa < 1:4585701/47‑1 (MQ=255) gTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTaa < 1:2577202/47‑1 (MQ=255) tAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATTAGCGTAAATTTGCTCAAc > 1:3823832/1‑57 (MQ=255) tAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActg > 1:539229/1‑59 (MQ=255) tAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAAc > 1:2467115/1‑57 (MQ=255) tAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAAc > 1:4422731/1‑57 (MQ=255) aaGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAAt > 1:4953038/1‑47 (MQ=255) aGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCg > 1:1105080/1‑70 (MQ=255) aGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGt < 1:2370097/71‑1 (MQ=255) aGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGt < 1:2363968/71‑1 (MQ=255) aGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGt < 1:1291981/71‑1 (MQ=255) aGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGt > 1:2696223/1‑71 (MQ=255) aGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGt < 1:3706142/71‑1 (MQ=255) aGCAACAGGCAAAGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGt < 1:4110697/71‑1 (MQ=255) aaCAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAActgccct > 1:3869740/1‑58 (MQ=255) aCAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATc > 1:3055351/1‑71 (MQ=255) cAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATcc > 1:2040645/1‑71 (MQ=255) aGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAAttt < 1:4764479/42‑1 (MQ=255) cAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATCCCAGc > 1:776586/1‑71 (MQ=255) aaGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCa < 1:2846247/43‑1 (MQ=255) aaGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATCCCAGCt < 1:4664233/71‑1 (MQ=255) aaGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATACCAGCt > 1:1137009/1‑71 (MQ=255) ggTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCaa > 1:4473356/1‑42 (MQ=255) ggTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATCCCAGCTgg < 1:3584033/71‑1 (MQ=255) ggTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATCCCAGCTgg < 1:1113646/71‑1 (MQ=255) | GATCCCCACCTCATCCCAGCTATACTGCGCCGCCAGCAGTTTACGCATCATGATGTAAGCAACAGGCAAGGTCACCGACGTAATCAACGCCATGAGCGTAAATTTGCTCAACTGCCCTGCCAGACCGTTATCCCAGCTGG > W3110S.gb/3659645‑3659784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |