| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,659,825 | C→T | 33.7% | M193I (ATG→ATA) | wzxE ← | O‑antigen translocase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,659,825 | 0 | C | T | 33.7% | 103.1 / 77.5 | 83 | M193I (ATG→ATA) | wzxE | O‑antigen translocase |
| Reads supporting (aligned to +/- strand): ref base C (29/26); new base T (18/10); total (47/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAACC > W3110S.gb/3659757‑3659893 | tgccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACat > 1:2332023/1‑71 (MQ=255)tgccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACat > 1:2207960/1‑71 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAatat < 1:3711095/69‑1 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAATatg < 1:4575095/70‑1 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACatg < 1:2997398/70‑1 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACatg < 1:2671543/70‑1 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACat < 1:3435942/69‑1 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACat < 1:1986423/69‑1 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACat < 1:1239406/69‑1 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACat < 1:656/69‑1 (MQ=255) ccctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGAAGACACCACGTTTGATCAatat < 1:3871767/69‑1 (MQ=255) cctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACatgat < 1:317731/71‑1 (MQ=255) ctgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAATatgatg > 1:4446574/1‑71 (MQ=255) tgccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAATATGATgg > 1:4476783/1‑71 (MQ=255) gccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACat < 1:2478552/65‑1 (MQ=255) ccAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAATATGATggcg > 1:1580838/1‑71 (MQ=255) ccGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAgg < 1:2949237/71‑1 (MQ=255) gTTATCCCAGCTTGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAgg < 1:4508758/69‑1 (MQ=255) ttATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGAAt > 1:2461565/1‑71 (MQ=255) cccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAatat < 1:3060970/52‑1 (MQ=255) ccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTacca > 1:3974076/1‑71 (MQ=255) ccAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTac > 1:2593613/1‑69 (MQ=255) cAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGaa > 1:4933822/1‑64 (MQ=255) cAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAAt > 1:1653031/1‑65 (MQ=255) cAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGAAGGAAt > 1:522018/1‑65 (MQ=255) gCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGAATTaccac > 1:1076454/1‑69 (MQ=255) tttCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTac < 1:2001956/60‑1 (MQ=255) tttCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTac < 1:2311397/60‑1 (MQ=255) tttCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTac < 1:2174911/60‑1 (MQ=255) tCAGATAGCTTAACGGGATGACACCACGTTTGATCAATatga > 1:2099164/1‑42 (MQ=255) tCAGATAGCTTAACGGGATGACACCACGTTTGATCAATatga > 1:439126/1‑42 (MQ=255) tCAGATAGCTTAACGGGATGACACCACGTTTGATCAATatga > 1:4092698/1‑42 (MQ=255) tCAGATAGCTTAACGGGATGACACCACGTTTGATCAATatga > 1:2932280/1‑42 (MQ=255) tCAGATAGCTTAACGGGATGACACCACGTTTGATCAATatga > 1:3550947/1‑42 (MQ=255) tCAGATAGCTTAACGGGATGACACCACGTTTGATCAATatga > 1:1951978/1‑42 (MQ=255) tCAGATAGCTTAACGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGaa > 1:4787143/1‑54 (MQ=255) tCAGATAGCTTAACGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGa > 1:210789/1‑53 (MQ=255) cAGATAGCTTAACGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGa < 1:3606420/70‑1 (MQ=255) cAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGaa > 1:4934100/1‑71 (MQ=255) cAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGaa > 1:4913912/1‑71 (MQ=255) aGATAGCTTAACGGGATGACACCACGTTTGATCAATatgat > 1:2580713/1‑41 (MQ=255) aGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGaa < 1:2660629/70‑1 (MQ=255) aGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAAt > 1:3251108/1‑71 (MQ=255) gATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATc < 1:4780517/71‑1 (MQ=255) aTAGCTTAACGGGATGACACCACGTTTGATCAatat < 1:4802421/36‑1 (MQ=255) aTAGCTTAACGGGATGACACCACGTTTGATCAatat < 1:3287049/36‑1 (MQ=255) aTAGCTTAACGGGATGACACCACGTTTGATCAatat < 1:1742025/36‑1 (MQ=255) aTAGCTTAACGGGATGACACCACGTTTGATCAatat < 1:1954879/36‑1 (MQ=255) aTAGCTTAACGGGATGACACCACGTTTGATCAACatga > 1:3861949/1‑38 (MQ=255) aTAGCTTAACGGGATGACACCACGTTTGATCAACatga > 1:2277115/1‑38 (MQ=255) tAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGa < 1:3206955/48‑1 (MQ=255) aGCTTAACGGGATGACACCACGTTTGATCAACATGATggc > 1:2864851/1‑40 (MQ=255) aGCTTAACGGGATGACACAACGTTTGATCAACATGATggc > 1:3341523/1‑40 (MQ=255) gCTTAACGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAgcg > 1:3320901/1‑71 (MQ=255) cTTAACGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAgcgc < 1:4742899/71‑1 (MQ=255) cTTAACGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAgcgc < 1:4737054/71‑1 (MQ=255) tAACGGGATGACACCACGTTTGATCAACATGATgg > 1:4834927/1‑35 (MQ=255) tAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAg > 1:1848747/1‑41 (MQ=255) aCGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGa < 1:913722/71‑1 (MQ=255) aCGGGATGACACCACGTTTGATCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGa < 1:2634332/71‑1 (MQ=255) gggATGACACCACGTTTGATCAACATGATGGCGGCAgg < 1:2523458/38‑1 (MQ=255) gggATGACACCACGTTTGATCAACATGATGGCGGCAgg < 1:4875633/38‑1 (MQ=255) gggATGACACCACGTTTGATCAACATGATGGCGGCAgg < 1:1640351/38‑1 (MQ=255) ggATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGAcc > 1:1069621/1‑70 (MQ=255) gATGACACCACGTTTGATCAACATGATGGCGGCAGGaa > 1:5018245/1‑38 (MQ=255) tGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGc < 1:4236567/71‑1 (MQ=255) gACACCACGTTTGATCAACATGATGGCGGCAGGAATTaccac > 1:2509262/1‑42 (MQ=255) caccacGTTTGATCAACATGATGGCGGCAGGAAtt > 1:3225498/1‑35 (MQ=255) accacGTTTGATCAATATGATGGCGGCAGGAATTac < 1:4844000/36‑1 (MQ=255) cgacGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGc > 1:745100/3‑71 (MQ=255) acGTTTGATCAACATGATGGCGGCAGGAATTACCACCAgcg > 1:477897/1‑41 (MQ=255) acGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAgcgc > 1:1718710/1‑54 (MQ=255) cGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGcccc > 1:2797520/1‑71 (MQ=255) cGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGcccc > 1:4532384/1‑71 (MQ=255) cGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGcccc > 1:3376680/1‑71 (MQ=255) gTTTGATCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGa < 1:4904138/44‑1 (MQ=255) tttGATCAACATGATGGCGGCAGGAATTACCACCAGCGCggg < 1:5146327/42‑1 (MQ=255) tttGATCAACATGATGGCGGCAGGAATTACCACCAGCGCggg < 1:1277701/42‑1 (MQ=255) tGATCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGAAACGCCCCTTc > 1:4719469/1‑70 (MQ=255) tGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCa > 1:3948932/1‑71 (MQ=255) tGATCAACATGATGGCGGCAGGAATTACCACCA‑‑GCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCAt < 1:4272056/70‑1 (MQ=255) aTCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAgcgc < 1:2789908/47‑1 (MQ=255) aTCAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCAt < 1:49203/70‑1 (MQ=255) aTCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCAt < 1:210228/70‑1 (MQ=255) aTCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCAt < 1:2475305/70‑1 (MQ=255) tCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGc < 1:4175535/56‑1 (MQ=255) cAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCaa > 1:3013313/1‑57 (MQ=255) cAATATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAAc > 1:744353/1‑71 (MQ=255) cAACATGATGGCGGGAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAAc > 1:4238576/1‑71 (MQ=255) aaCATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAAcc > 1:3049373/1‑71 (MQ=255) | TGCCCTGCCAGACCGTTATCCCAGCTGGGTTTCAGATAGCTTAACGGGATGACACCACGTTTGATCAACATGATGGCGGCAGGAATTACCACCAGCGCGGGAATCAGCGCCAGACCCAGCAACGCCCCTTCATAACC > W3110S.gb/3659757‑3659893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |