| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,733,360 | T→C | 42.1% | Y127H (TAC→CAC) | bglB → | cryptic phospho‑beta‑glucosidase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,733,360 | 0 | T | C | 42.1% | 24.8 / 72.2 | 57 | Y127H (TAC→CAC) | bglB | cryptic phospho‑beta‑glucosidase B |
| Reads supporting (aligned to +/- strand): ref base T (18/15); new base C (7/17); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCGATCACTTC > W3110S.gb/3733294‑3733428 | ttttACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGa < 1:3351746/71‑1 (MQ=255) ttACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGaaa > 1:980426/1‑71 (MQ=255) cGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGc < 1:1491733/71‑1 (MQ=255) aTCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCa < 1:357138/71‑1 (MQ=255) aTCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCa < 1:1396377/71‑1 (MQ=255) tCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCa > 1:1292171/1‑70 (MQ=255) ggCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCatat > 1:2529612/1‑71 (MQ=255) ggCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCatat > 1:4657288/1‑71 (MQ=255) gTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGcc < 1:236467/63‑1 (MQ=255) ttGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTg > 1:4805294/1‑71 (MQ=255) tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGaaa > 1:1029539/1‑56 (MQ=255) tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGaaa > 1:4265093/1‑56 (MQ=255) tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTgg > 1:2806170/1‑71 (MQ=255) tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTgg > 1:1725629/1‑71 (MQ=255) tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTgg > 1:852095/1‑71 (MQ=255) gaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTa > 1:1116585/1‑47 (MQ=255) gaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGa > 1:1197942/1‑70 (MQ=255) gaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGa > 1:13654/1‑70 (MQ=255) gaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACg < 1:799796/49‑1 (MQ=255) gaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGa > 1:2927973/1‑70 (MQ=255) aaaTGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGaa < 1:671734/70‑1 (MQ=255) aaaTGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGaa < 1:78186/70‑1 (MQ=255) tGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGa < 1:3764160/46‑1 (MQ=255) ggCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGa < 1:3632303/45‑1 (MQ=255) cgcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACg < 1:744616/42‑1 (MQ=255) gCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAcggcgg < 1:227193/71‑1 (MQ=255) gCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGaaa < 1:1203605/60‑1 (MQ=255) gCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGAAAAAc < 1:89774/63‑1 (MQ=255) gCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGAAAAAc < 1:2400370/63‑1 (MQ=255) cGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGt > 1:2267692/1‑71 (MQ=255) cGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGt > 1:478277/1‑71 (MQ=255) gggATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTg < 1:4351255/71‑1 (MQ=255) ggATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTgg > 1:3918598/1‑71 (MQ=255) gATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTggg > 1:3182610/1‑71 (MQ=255) gATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGc < 1:1500513/38‑1 (MQ=255) gATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGc < 1:4241377/38‑1 (MQ=255) gATCAAGCCGCTGGTAACGTTATCACATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGtt > 1:1093818/1‑68 (MQ=255) aTCTAGCCGCTGGTTACGTTATCCCATCACGAAATGCCat < 1:4167217/40‑1 (MQ=25) aTCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCa < 1:2636379/39‑1 (MQ=255) aTCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCat < 1:4118240/40‑1 (MQ=255) aTCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCat < 1:2204762/40‑1 (MQ=255) aTCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCat < 1:4820027/40‑1 (MQ=255) aTCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGaaa < 1:2847059/54‑1 (MQ=255) tCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCat < 1:3059610/39‑1 (MQ=255) tCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGc > 1:39062/1‑70 (MQ=255) cAAGCCTCTGGTAACGTTATCCCATCACGAAATGCCat < 1:3847488/38‑1 (MQ=38) cAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGAAAAACTAcggcgg > 1:2538039/1‑63 (MQ=255) aaGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTaa > 1:402247/1‑71 (MQ=255) aaGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTa < 1:2820148/70‑1 (MQ=255) gCTGGTAACGTTATCCCATTACGAATTGCCATATGGGCTGGTGAAAAACTACGGCGGt < 1:49250/58‑1 (MQ=255) gTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAc > 1:915589/1‑43 (MQ=255) ttATCCCATCACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATc < 1:2652405/69‑1 (MQ=255) ttATCCCATCACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATc < 1:2706169/69‑1 (MQ=255) ttATCCCATCACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCGa < 1:1880752/71‑1 (MQ=255) aTCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGc > 1:4120108/1‑51 (MQ=255) aTCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCGAt < 1:4609131/70‑1 (MQ=255) aTTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCGATCACTTc > 1:918917/1‑71 (MQ=255) ttACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCgg < 1:3849112/56‑1 (MQ=255) ttACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCa > 1:1607211/1‑59 (MQ=255) tACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAAt > 1:73717/1‑48 (MQ=255) tACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCg < 1:512599/61‑1 (MQ=255) | TTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCGATCACTTC > W3110S.gb/3733294‑3733428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |