| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,735,429 | G→A | 24.3% | A323T (GCA→ACA) | bglH → | carbohydrate‑specific outer membrane porin, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,735,429 | 0 | G | A | 24.3% | 230.5 / 71.8 | 115 | A323T (GCA→ACA) | bglH | carbohydrate‑specific outer membrane porin, cryptic |
| Reads supporting (aligned to +/- strand): ref base G (28/59); new base A (14/14); total (42/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
TAAAGATATCCCGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGCA > W3110S.gb/3735359‑3735495 | ccGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGaaaa < 1:4610366/71‑1 (MQ=255) ccGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGaaa > 1:849155/1‑70 (MQ=255) aTGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGAt > 1:1773937/1‑70 (MQ=255) tAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAgg < 1:4525791/71‑1 (MQ=255) aaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGa < 1:367304/61‑1 (MQ=255) aaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGa > 1:3024582/1‑70 (MQ=255) aaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGa < 1:3223853/70‑1 (MQ=255) aaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGa > 1:2429962/1‑70 (MQ=255) aGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGata < 1:4837451/71‑1 (MQ=255) aGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGata < 1:1691782/71‑1 (MQ=255) aGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGa < 1:3925143/69‑1 (MQ=255) gCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGata < 1:2819653/70‑1 (MQ=255) gCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGataa > 1:139008/1‑71 (MQ=255) ccACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataat < 1:2347771/71‑1 (MQ=255) ccACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataat < 1:4677896/71‑1 (MQ=255) ccACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataat < 1:567058/71‑1 (MQ=255) ccACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataat < 1:1809687/71‑1 (MQ=255) ccACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataat < 1:2341677/71‑1 (MQ=255) cACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGataat < 1:2301548/70‑1 (MQ=255) ccTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAAc < 1:4140110/71‑1 (MQ=255) cTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataa < 1:3291107/66‑1 (MQ=255) cTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataa < 1:3543534/66‑1 (MQ=255) aaTGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:771389/70‑1 (MQ=255) aaTGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACgg < 1:3190855/69‑1 (MQ=255) aTGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:765330/69‑1 (MQ=255) gTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtatt < 1:518105/70‑1 (MQ=255) gTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATc > 1:3825163/1‑38 (MQ=255) tAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:4574955/71‑1 (MQ=255) tAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:1410845/71‑1 (MQ=255) tAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:4316699/71‑1 (MQ=255) tAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:316465/71‑1 (MQ=255) tAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:3135358/71‑1 (MQ=255) aaGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGaa > 1:191460/1‑40 (MQ=255) aaGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:3515719/70‑1 (MQ=255) gTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGa < 1:2224546/37‑1 (MQ=255) gTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGa < 1:3860887/37‑1 (MQ=255) ggTCGTTATGTCACGGCAAACGAAAGCGCATCGGaaa > 1:4278911/1‑37 (MQ=255) ggTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGAtaataa > 1:3857193/1‑56 (MQ=255) ggTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg > 1:5007921/1‑71 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATa > 1:557458/1‑42 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATa > 1:2705211/1‑42 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATc < 1:419041/45‑1 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATc < 1:2896518/45‑1 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATc < 1:538522/45‑1 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg < 1:790406/70‑1 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg > 1:2320833/1‑70 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg < 1:3637152/70‑1 (MQ=255) cGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATATTCa > 1:1023851/1‑44 (MQ=255) cGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATAAg > 1:4201281/1‑45 (MQ=255) gTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACTGGTATTATGACTGGa > 1:2061286/1‑69 (MQ=255) gTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGa > 1:5054673/1‑64 (MQ=255) ttATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATa < 1:4559614/38‑1 (MQ=255) ttATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAAt < 1:3238433/40‑1 (MQ=255) ttATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGaaa < 1:3230877/70‑1 (MQ=255) ttATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtat < 1:4807968/58‑1 (MQ=255) aTGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:2763232/53‑1 (MQ=255) aTGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg > 1:1216930/1‑60 (MQ=255) aTGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGa > 1:2061584/1‑61 (MQ=255) aTGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGa < 1:3594183/70‑1 (MQ=255) aTGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGa > 1:1993160/1‑70 (MQ=255) tGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATa < 1:1308821/71‑1 (MQ=255) tGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATa < 1:2631292/71‑1 (MQ=255) tGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATa < 1:2625514/71‑1 (MQ=255) gTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGa < 1:1868439/68‑1 (MQ=255) gTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGa < 1:2405655/68‑1 (MQ=255) gTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGataat < 1:1451666/45‑1 (MQ=255) tCACGGCAAACGAAAGCGCATCGGAAAAAGATAAt < 1:735396/35‑1 (MQ=255) tCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatta > 1:2628303/1‑55 (MQ=255) tCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatta < 1:2014134/55‑1 (MQ=255) cACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCt > 1:2778607/1‑71 (MQ=255) aCGGCAAACGAAAGCGCATCGGAAAAAGATAATCAg > 1:4038271/1‑36 (MQ=255) aCGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtattat > 1:4968455/1‑54 (MQ=255) aCGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtattat > 1:1381907/1‑54 (MQ=255) aCGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:4212151/54‑1 (MQ=255) aCGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTg > 1:1627094/1‑71 (MQ=255) ggCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAAc > 1:277652/1‑43 (MQ=255) gCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGa > 1:188217/1‑70 (MQ=255) cAAACGAAAGCGCATCGGAAAAAGATAATCATGATAATAACGGGTATTATGACTGGAAAGATACCTGGATg < 1:1318288/71‑1 (MQ=255) cAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATg < 1:4573515/71‑1 (MQ=255) cAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATg < 1:3096753/71‑1 (MQ=255) aaaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGt < 1:76130/71‑1 (MQ=255) aaCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtatt < 1:2624730/46‑1 (MQ=255) aaCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGt < 1:4764073/70‑1 (MQ=255) aaCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACg > 1:4485778/1‑40 (MQ=255) aCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtat < 1:1417986/44‑1 (MQ=255) aCGAAAGCACATCGGAAAAAGATAATCAGGAtaataa > 1:3551284/1‑37 (MQ=255) cGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:2246813/40‑1 (MQ=255) cGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:3013207/40‑1 (MQ=255) cGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtattat > 1:1460860/1‑46 (MQ=255) cGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtattat > 1:3201227/1‑46 (MQ=255) cGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTg < 1:1529203/63‑1 (MQ=255) cGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTg < 1:2589735/63‑1 (MQ=255) cGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTg < 1:2191107/63‑1 (MQ=255) cGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtattat > 1:3269955/1‑46 (MQ=255) cGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtattat > 1:1220238/1‑46 (MQ=255) gAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGaaa < 1:2736343/54‑1 (MQ=255) aaGCGCATCGGAAAAAGATAATCAGGTTAATAACGGGTATTATGACt > 1:4589217/1‑47 (MQ=255) aaGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACt > 1:3891224/1‑47 (MQ=255) aaGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGca < 1:5120117/71‑1 (MQ=255) aaGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGca < 1:3168397/71‑1 (MQ=255) | TAAAGATATCCCGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGCA > W3110S.gb/3735359‑3735495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |