Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 275,785 G→A 42.0% V413I (GTA→ATA)  mmuP → predicted S‑methylmethionine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb275,7850GA42.0% 27.5 / 59.1 50V413I (GTA→ATA) mmuPpredicted S‑methylmethionine transporter
Reads supporting (aligned to +/- strand):  ref base G (19/10);  new base A (13/8);  total (32/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

TGCAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGT  >  W3110S.gb/275723‑275854
                                                              |                                                                     
tGCAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGt                                                               >  1:1054549/1‑71 (MQ=255)
tGCAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGt                                                               >  1:2267282/1‑71 (MQ=255)
 gCAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGtt                                                              >  1:2285559/1‑71 (MQ=255)
          tAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtgtgctgt                                                     >  1:2293171/1‑71 (MQ=255)
          tAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtgtgctgt                                                     >  1:4907845/1‑71 (MQ=255)
              gCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCt                                                 <  1:3107480/71‑1 (MQ=255)
              gCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCt                                                 <  1:386839/71‑1 (MQ=255)
                 ttGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGcc                                                  <  1:1443813/67‑1 (MQ=255)
                 ttGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCtggt                                              <  1:2540284/71‑1 (MQ=255)
                   gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtgtgctgtgc                                                   >  1:781878/1‑64 (MQ=255)
                   gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCtggtgg                                            >  1:4028700/1‑71 (MQ=255)
                      tGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtgtgat                                                       >  1:2485386/1‑55 (MQ=255)
                      tGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCt                                         >  1:4689511/1‑71 (MQ=255)
                        aaTTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTAtt                                                                   <  1:362552/43‑1 (MQ=255)
                        aaTTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCtgt                                       >  1:1485202/1‑71 (MQ=255)
                        aaTTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCtgt                                       >  1:2793376/1‑71 (MQ=255)
                         aTTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgcgct                                                       <  1:655463/54‑1 (MQ=39)
                          ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtgtgc                                                        <  1:655646/52‑1 (MQ=255)
                            aCATTATCGCGCGCAGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg                                                         >  1:2604393/1‑49 (MQ=39)
                             cATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTa                                                                  >  1:195427/1‑39 (MQ=255)
                             cATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTa                                                                  >  1:2226506/1‑39 (MQ=255)
                             cATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTg                                   <  1:42253/70‑1 (MQ=255)
                              aTTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCtgt                                       <  1:116030/65‑1 (MQ=255)
                              aTTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCtgt                                       <  1:1599025/65‑1 (MQ=255)
                                tATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgctgtg                                                    >  1:4158305/1‑50 (MQ=255)
                                tATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGc                                <  1:1754473/70‑1 (MQ=255)
                                tATCGCGCGCCGTGGTATCCGCTGGTGCAAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCt                               >  1:239084/1‑71 (MQ=255)
                                 aTCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtg                                                           >  1:4953362/1‑42 (MQ=255)
                                 aTCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtg                                                           >  1:4374200/1‑42 (MQ=255)
                                 aTCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtg                                                           >  1:3657807/1‑42 (MQ=255)
                                 aTCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtg                                                           >  1:4061446/1‑42 (MQ=255)
                                 aTCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTg                              >  1:5060172/1‑71 (MQ=255)
                                 aTCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTg                              >  1:1651294/1‑71 (MQ=255)
                                 aTCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTg                              >  1:2009773/1‑71 (MQ=255)
                                  tCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTgg                             <  1:191289/71‑1 (MQ=255)
                                  tCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgctg                                                      >  1:1424655/1‑46 (MQ=255)
                                       cgcCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTc                        >  1:2329338/1‑71 (MQ=255)
                                         ccGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGa                      >  1:342632/1‑71 (MQ=255)
                                          cGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGAt                     >  1:2765246/1‑71 (MQ=255)
                                            tGGTATCCGCTGGTGCCAGTATTAGGTTTtgtgctgt                                                     <  1:2992893/37‑1 (MQ=255)
                                                 tCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAgcg               <  1:1467473/70‑1 (MQ=255)
                                                   cGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGc                                           >  1:2832826/1‑40 (MQ=255)
                                                     cTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCa             >  1:1979707/1‑68 (MQ=255)
                                                      tGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTggg                                 >  1:2717031/1‑47 (MQ=255)
                                                      tGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCagag          >  1:4307636/1‑70 (MQ=255)
                                                      tGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCagag          >  1:4833038/1‑70 (MQ=255)
                                                        gTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAAt       <  1:3446387/71‑1 (MQ=255)
                                                        gTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAgaga         <  1:4987874/69‑1 (MQ=255)
                                                        gTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAgaga         <  1:2630249/69‑1 (MQ=255)
                                                             aGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGt  <  1:2614559/71‑1 (MQ=255)
                                                              |                                                                     
TGCAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGT  >  W3110S.gb/275723‑275854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: