| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,790,027 | C→A | 13.4% | H113N (CAT→AAT) | uhpA → | DNA‑binding response regulator in two‑component regulatory system wtih UhpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,790,027 | 0 | C | A | 13.4% | 111.3 / 3.9 | 37 | H113N (CAT→AAT) | uhpA | DNA‑binding response regulator in two‑component regulatory system wtih UhpB |
| Reads supporting (aligned to +/- strand): ref base C (14/18); new base A (3/2); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
GCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTGGCATCCGG > W3110S.gb/3789960‑3790088 | gcTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCa < 1:3064988/69‑1 (MQ=255) cTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATAc < 1:2890754/71‑1 (MQ=255) ggggCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCAc < 1:3472276/71‑1 (MQ=255) ctttctttCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCa < 1:2191621/48‑1 (MQ=255) tttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTa < 1:556393/67‑1 (MQ=255) tttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTa < 1:3068166/67‑1 (MQ=255) tttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTa < 1:2513949/67‑1 (MQ=255) ttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGt < 1:1004987/48‑1 (MQ=255) ttcCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGt < 1:2572852/48‑1 (MQ=255) ttcCAAACGCTGTAGCACGGATGAACTCATTGCTGCGGTGCATACGGt < 1:4108336/48‑1 (MQ=255) aCGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCgg < 1:4159580/71‑1 (MQ=255) cGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCa > 1:1542442/1‑35 (MQ=255) cGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCa > 1:3525813/1‑35 (MQ=255) cTGTAGCCCGGATGAACTCATTGCTGCGGTGAATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGata < 1:1576683/71‑1 (MQ=255) tAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTg < 1:1275915/71‑1 (MQ=255) cGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGa < 1:242560/61‑1 (MQ=255) ggATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATc > 1:1808193/1‑50 (MQ=255) ggATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCgg < 1:1668181/59‑1 (MQ=255) ggATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCgg < 1:2444478/59‑1 (MQ=255) ggATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCAtt > 1:4528484/1‑70 (MQ=255) ggATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTa < 1:51448/71‑1 (MQ=255) gATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTaa < 1:4647864/71‑1 (MQ=255) tGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTaaa > 1:787904/1‑70 (MQ=255) tGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAAc > 1:3772466/1‑71 (MQ=255) aaCTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTg > 1:4358126/1‑71 (MQ=255) aaCTCATTGCTGCGGTGAATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTg < 1:4361734/71‑1 (MQ=255) cATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGata > 1:3918906/1‑53 (MQ=255) cATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGata > 1:3929702/1‑53 (MQ=255) cATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGata > 1:2021348/1‑53 (MQ=255) aTTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTa > 1:3398492/1‑38 (MQ=255) aTTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTa > 1:1232286/1‑38 (MQ=255) aTTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTGGCATc > 1:5090720/1‑71 (MQ=255) aTTGCTGCGGTGAATACGGTTGCCACGGGCGGCTGTTATCTGa > 1:1115233/1‑43 (MQ=255) aTTGCTGCGGTGAATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTGGCAt > 1:3550722/1‑70 (MQ=255) gctgcGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTCCCATTAAACTGGCATCCgg > 1:1523905/1‑71 (MQ=255) gctgcGGTGAATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTGGCATCCgg > 1:1141720/1‑71 (MQ=255) gTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTGGCATCCg < 1:2003314/64‑1 (MQ=255) cATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTa > 1:3340847/1‑49 (MQ=255) | GCTTAACGCGGGGGCACGCGGCTTTCTTTCCAAACGCTGTAGCCCGGATGAACTCATTGCTGCGGTGCATACGGTTGCCACGGGCGGCTGTTATCTGACGCCGGATATTGCCATTAAACTGGCATCCGG > W3110S.gb/3789960‑3790088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |