| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,806,473 | G→A | 35.4% | P198P (CCG→CCA) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,806,473 | 0 | G | A | 35.4% | 70.8 / 62.9 | 65 | P198P (CCG→CCA) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base G (22/20); new base A (12/11); total (34/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATGGCCAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTGG > W3110S.gb/3806403‑3806540 | aTGGCCAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCg > 1:3948251/1‑71 (MQ=255) gCCAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTc < 1:4975222/71‑1 (MQ=255) tAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGa > 1:273062/1‑69 (MQ=255) tAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACt < 1:210408/71‑1 (MQ=255) agaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAc < 1:1621763/71‑1 (MQ=255) aCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCg > 1:5135912/1‑71 (MQ=255) cTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGt < 1:390461/70‑1 (MQ=255) gAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCg < 1:2870295/66‑1 (MQ=255) gAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGt < 1:354195/70‑1 (MQ=255) gAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGt > 1:3458850/1‑70 (MQ=255) ccGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg < 1:4040327/71‑1 (MQ=255) cGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATgg < 1:2186351/71‑1 (MQ=255) cGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATgg < 1:3710193/71‑1 (MQ=255) gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGT‑‑TACATGACTAACCGTGGTTa > 1:3231538/1‑65 (MQ=255) ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTgg < 1:291736/63‑1 (MQ=255) ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTgg < 1:2108997/63‑1 (MQ=255) aCGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTAt > 1:2537105/1‑65 (MQ=255) cggcggCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTAc < 1:115499/71‑1 (MQ=255) ggcggcACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTgg < 1:2546592/59‑1 (MQ=255) cacaAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATggg > 1:2171082/1‑61 (MQ=255) cacaAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTc > 1:2262370/1‑70 (MQ=255) aGTACTGAACAGGCGTATAAAAATATCCCGTTCTACa > 1:2040694/1‑37 (MQ=255) gTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTa > 1:2832984/1‑52 (MQ=255) cTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATccc > 1:1331092/1‑71 (MQ=255) cTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATcc < 1:1444472/70‑1 (MQ=255) cTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatca > 1:2830165/1‑67 (MQ=255) gAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATccc > 1:4282163/1‑69 (MQ=255) gAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATccc > 1:1380932/1‑69 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAg > 1:1487007/1‑71 (MQ=255) cAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatca > 1:3636316/1‑62 (MQ=255) cGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtg > 1:3975530/1‑67 (MQ=255) tataAAAATATCCCGTTCTACATGACTAACCGTGGt < 1:2232354/36‑1 (MQ=255) tataAAAATATCCCGTTCTACATGACTAACCGTGGTTATgg < 1:1622285/41‑1 (MQ=255) tataAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatc < 1:4650590/55‑1 (MQ=255) tataAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatc < 1:3499814/55‑1 (MQ=255) tataAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATccc < 1:1215212/60‑1 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTAt < 1:356402/37‑1 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtt > 1:1355750/1‑71 (MQ=255) taAAAATATCCCATTCTACATGACTAACCGTGGTTATggg > 1:3566825/1‑40 (MQ=255) taAAAATATCCCATTCTACATGACTAACCGTGGTTATggg > 1:2553864/1‑40 (MQ=255) taAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTAc > 1:3252852/1‑44 (MQ=255) taAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatca > 1:3324824/1‑54 (MQ=255) taAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTctct < 1:2421446/70‑1 (MQ=255) taAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtt < 1:4288921/71‑1 (MQ=255) taAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtt < 1:2676080/71‑1 (MQ=255) taAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtt < 1:4027480/71‑1 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCa > 1:807211/1‑58 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtc < 1:3442298/65‑1 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtc < 1:2267130/65‑1 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtttt > 1:2309711/1‑71 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtttt > 1:1184936/1‑71 (MQ=255) aaaaTATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatcat > 1:4506607/1‑53 (MQ=255) aaaaTATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatcat > 1:46553/1‑53 (MQ=255) aaaaTATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatcat > 1:5106277/1‑53 (MQ=255) aaaaTATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatcat > 1:3471777/1‑53 (MQ=255) aaaaTATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtt < 1:1475942/69‑1 (MQ=255) aaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtg > 1:129895/1‑60 (MQ=255) aaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTg < 1:5037662/70‑1 (MQ=255) aaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGa < 1:996157/71‑1 (MQ=255) aaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGa < 1:821623/71‑1 (MQ=255) aaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGa < 1:1122978/71‑1 (MQ=255) tCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatc > 1:4921650/1‑45 (MQ=255) tCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatc > 1:814514/1‑45 (MQ=255) tCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatc > 1:1539815/1‑45 (MQ=255) cccGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTgg > 1:4505054/1‑71 (MQ=255) cccGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTgg > 1:4242803/1‑71 (MQ=255) | ATGGCCAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTGG > W3110S.gb/3806403‑3806540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |