| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,815,599 | C→T | 5.4% | A102T (GCT→ACT) | trmH ← | tRNA (Guanosine‑2'‑O‑)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,815,599 | 0 | C | T | 5.4% | 306.7 / 5.1 | 93 | A102T (GCT→ACT) | trmH | tRNA (Guanosine‑2'‑O‑)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (41/47); new base T (2/3); total (43/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 | |||||||||||
ATGCCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGCCAA > W3110S.gb/3815529‑3815667 | aTGCCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGc < 1:1930717/71‑1 (MQ=255) tGCCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCg < 1:2624752/71‑1 (MQ=255) cccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGtt < 1:409561/71‑1 (MQ=255) ttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAg > 1:1034369/1‑70 (MQ=255) ttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAg > 1:1897213/1‑70 (MQ=255) ctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGa > 1:241273/1‑69 (MQ=255) ctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGa > 1:3307050/1‑69 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTAt > 1:3351157/1‑62 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaaa < 1:240297/68‑1 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaaa < 1:1646649/68‑1 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGa < 1:1791963/70‑1 (MQ=255) tCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATggg < 1:1396301/71‑1 (MQ=255) tCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATggg < 1:1666039/71‑1 (MQ=255) ccATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGt > 1:179488/1‑54 (MQ=255) ccATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGt > 1:1813038/1‑54 (MQ=255) cATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAATATGGGTTg < 1:2946003/71‑1 (MQ=255) aTCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaaa < 1:4610906/61‑1 (MQ=255) tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGtt < 1:3220812/68‑1 (MQ=255) tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGcc < 1:2657149/71‑1 (MQ=255) tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:2657506/1‑70 (MQ=255) tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:2401113/1‑70 (MQ=255) tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:2621403/1‑70 (MQ=255) tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:1798212/1‑70 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:1225200/1‑68 (MQ=255) aaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGa < 1:4067132/71‑1 (MQ=255) aaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGaa < 1:3048786/71‑1 (MQ=255) aaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGaa > 1:50980/1‑71 (MQ=255) aTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAAt > 1:309271/1‑71 (MQ=255) tGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAAt > 1:2273392/1‑70 (MQ=255) tGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAAt > 1:935102/1‑70 (MQ=255) cAGGTCGGGCGAGTGTAATCATTTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt < 1:3893644/70‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:3900000/71‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:165601/71‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt < 1:2852185/70‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt < 1:2846458/70‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:690970/71‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:104663/71‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTGGCCAGAATCTg < 1:4050629/71‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGCTGCCAGAATCTg < 1:4949764/71‑1 (MQ=255) aGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:795620/70‑1 (MQ=255) aGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGc < 1:3181079/71‑1 (MQ=255) gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATc < 1:3241449/66‑1 (MQ=255) cGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATggg < 1:836061/53‑1 (MQ=255) cGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGt > 1:2169631/1‑54 (MQ=255) cGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:1405264/62‑1 (MQ=255) aGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaaa < 1:2279485/41‑1 (MQ=255) tgtAATCAATTTCGCGGAAATCGACAGCGTTATCATaaa < 1:2220691/39‑1 (MQ=255) tgtAATCAATTTCGCGGAAATCGACAGCGTTATCAGa < 1:3878933/37‑1 (MQ=255) tAATCAATTTCGCGGAAATCGACATCGTTATCAGAAAGATGGGTTGCCAGa < 1:2633481/51‑1 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGa < 1:425609/51‑1 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt > 1:3677458/1‑55 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt > 1:1383863/1‑55 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt > 1:3952570/1‑55 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt > 1:2335760/1‑55 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGAt < 1:2057105/38‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGAt < 1:2653805/38‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGAt < 1:1400106/38‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGAt < 1:44661/38‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGAt < 1:4405116/38‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATTGGTTGCCAGAATCTGCATGCCCTGGCCTTTga < 1:350550/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCAt > 1:1558642/1‑57 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCtat > 1:847809/1‑67 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga < 1:3421119/71‑1 (MQ=255) tCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGAt < 1:2020700/37‑1 (MQ=255) cAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATgg > 1:4704817/1‑38 (MQ=255) cAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATgg > 1:4543519/1‑38 (MQ=255) aaTTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGcc > 1:4110449/1‑43 (MQ=255) aaTTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGcc > 1:4553234/1‑43 (MQ=255) aaTTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGAt < 1:3032613/71‑1 (MQ=255) ttCGCGGAAATCGACAGCGTTATCAGAAAGATggg > 1:287817/1‑35 (MQ=255) ttCGCGGAAATCGACAGCGTTATCAGAAAGATggg > 1:2229424/1‑35 (MQ=255) ttCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGa < 1:1349956/43‑1 (MQ=255) ttCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGa < 1:3182711/43‑1 (MQ=255) ttCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGa < 1:4483216/43‑1 (MQ=255) tCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGc > 1:4349817/1‑71 (MQ=255) ggAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACg > 1:3264428/1‑71 (MQ=255) gAAATCGACAGCGTTATCTGAAAGATGGGTTGCCAGAATc < 1:639859/40‑1 (MQ=255) gAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt < 1:119948/57‑1 (MQ=255) aaaTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACgg > 1:3338038/1‑70 (MQ=255) aaaTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACgg > 1:3331875/1‑70 (MQ=255) aaaTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACgg > 1:2246982/1‑70 (MQ=255) aaaTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGc < 1:986419/71‑1 (MQ=255) aaaTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGc < 1:2437552/71‑1 (MQ=255) aTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTgaga > 1:1817869/1‑58 (MQ=255) aTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTgaga > 1:1895604/1‑58 (MQ=255) aTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCAt > 1:185646/1‑71 (MQ=255) cGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCg > 1:3857291/1‑71 (MQ=255) cGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCg > 1:3260265/1‑71 (MQ=255) cGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCg > 1:3239672/1‑71 (MQ=255) aCAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGc > 1:4289162/1‑70 (MQ=255) aCAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGc > 1:1978934/1‑70 (MQ=255) aCAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGc > 1:94566/1‑70 (MQ=255) aGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGCCaa < 1:4858168/71‑1 (MQ=255) aGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGCCaa < 1:2896827/71‑1 (MQ=255) cGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGcc < 1:3919083/67‑1 (MQ=255) cGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGcc < 1:3913335/67‑1 (MQ=255) cGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGcc < 1:3268605/67‑1 (MQ=255) | ATGCCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGCCAA > W3110S.gb/3815529‑3815667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |