| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 281,512 | C→T | 35.1% | S11F (TCC→TTC) | yagE → | predicted lyase/synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 281,512 | 0 | C | T | 35.1% | 62.3 / 54.9 | 57 | S11F (TCC→TTC) | yagE | predicted lyase/synthase |
| Reads supporting (aligned to +/- strand): ref base C (20/17); new base T (9/11); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAA > W3110S.gb/281444‑281578 | ttAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcg < 1:1113284/71‑1 (MQ=255) taaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTcgc > 1:2613515/1‑71 (MQ=255) taaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTcgc > 1:1564438/1‑71 (MQ=255) taaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcgc > 1:4561368/1‑71 (MQ=255) taaataaaCGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCcg < 1:2130411/70‑1 (MQ=255) aataaaCGAACGCTGTTCTATAATGTACAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgt > 1:2937744/1‑71 (MQ=255) aacgaacgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCgttgtt < 1:440020/71‑1 (MQ=255) aacgaacgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCgttgtt < 1:4134836/71‑1 (MQ=255) aacgaacgCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCgttgtt < 1:1321301/71‑1 (MQ=255) cTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGaa > 1:2537110/1‑71 (MQ=255) ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGAATc < 1:3936171/70‑1 (MQ=255) ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGAATc < 1:4599203/70‑1 (MQ=255) ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGAATc < 1:1060852/70‑1 (MQ=255) ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAAt < 1:3051892/69‑1 (MQ=255) ttCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAAt < 1:2500371/69‑1 (MQ=255) tCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGAATCa > 1:1722652/1‑70 (MQ=255) tCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCAt > 1:4330783/1‑71 (MQ=255) taATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTc > 1:2684504/1‑56 (MQ=255) aTGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCa < 1:3098020/64‑1 (MQ=255) aTGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCa < 1:3939169/64‑1 (MQ=255) tGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCt < 1:506249/71‑1 (MQ=255) aGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGtc > 1:156529/1‑71 (MQ=255) gAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCa > 1:4959120/1‑50 (MQ=255) gAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCa > 1:3861728/1‑59 (MQ=255) cAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCa > 1:3855969/1‑47 (MQ=255) ttCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttttt < 1:4498779/71‑1 (MQ=255) ttCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttttt < 1:478625/71‑1 (MQ=255) tCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACg > 1:1803/1‑40 (MQ=255) gCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCAc > 1:633848/1‑36 (MQ=255) gCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCAc > 1:323982/1‑36 (MQ=255) gCAAGGAGATCTCATGCCGCAGTTCGCGTTGTTCAc > 1:185258/1‑36 (MQ=255) cAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTccacca > 1:138058/1‑61 (MQ=255) cAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTccacca > 1:2861817/1‑61 (MQ=255) cAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTccacca > 1:2002334/1‑61 (MQ=255) aGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGaa > 1:322524/1‑38 (MQ=255) aGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGAATCATTCCCCCt < 1:1430523/49‑1 (MQ=255) aGGAGATCTCATGCCGCAGTTCGCGTTGTTCACGGGAATCATTCCCCCTGtctc > 1:1450580/1‑54 (MQ=255) ggAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTccacca < 1:2376831/58‑1 (MQ=255) ggAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTAccgcc < 1:3624436/69‑1 (MQ=255) ggAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTAccgcc < 1:4778421/69‑1 (MQ=255) gagaTCTCATGCCGCAGTTCGCGTTGTTCACGGGAATCATTccc > 1:606002/1‑44 (MQ=255) agaTCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACg > 1:4815429/1‑71 (MQ=255) aTCTCATGCCGCAGTTCGCGTTGTTCACGGGAATCATTCCCCCt < 1:607206/44‑1 (MQ=255) aTCTCATGCCGCAGTTCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttttt < 1:3432388/59‑1 (MQ=255) aTCTCATGCCGCAGTTCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttttt < 1:4721943/59‑1 (MQ=255) aTCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTccacca < 1:3519482/54‑1 (MQ=255) ctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAtttt > 1:2089980/1‑56 (MQ=255) ctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCAttt > 1:3672794/1‑55 (MQ=255) ctcATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGccg > 1:3607966/1‑70 (MQ=255) tcATGCCGCAGTCCGCGTTGTTCACGGGAATCAtt > 1:2145923/1‑35 (MQ=255) tcATGCCGCAGTCCGCGTTGTTCACGGGAATCAtt > 1:3441225/1‑35 (MQ=255) cATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAg > 1:3964814/1‑70 (MQ=255) aTGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGcc < 1:240697/67‑1 (MQ=255) tGCCGCAGTTCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTc < 1:2319499/71‑1 (MQ=255) tGCCGCAGTTCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTc < 1:403982/71‑1 (MQ=255) ccGCAGTCCGCGTTGTTCAGGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGa < 1:1755981/71‑1 (MQ=255) cAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATaa < 1:2723815/71‑1 (MQ=255) | TTAAATAAACGAACGCTGTTCTATAATGTAGAACAAAATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAA > W3110S.gb/281444‑281578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |