| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,970 | G→A | 5.9% | G108S (GGC→AGC) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,970 | 0 | G | A | 5.9% | 278.1 / 5.0 | 84 | G108S (GGC→AGC) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (34/45); new base A (3/2); total (37/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGG > W3110S.gb/3824901‑3825037 | cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAgg < 1:4897471/71‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg < 1:1372096/70‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAg > 1:3027003/1‑67 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATc > 1:809945/1‑70 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATc > 1:3382597/1‑70 (MQ=255) catcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCt < 1:4412349/71‑1 (MQ=255) atcatGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtg < 1:535606/71‑1 (MQ=255) atGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcggc > 1:2629154/1‑62 (MQ=255) atGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcggc > 1:1490569/1‑62 (MQ=255) atGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcggc > 1:3800021/1‑62 (MQ=255) atGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcggc > 1:3487678/1‑62 (MQ=255) atGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg > 1:4223994/1‑60 (MQ=255) atGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg > 1:708664/1‑60 (MQ=255) atGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg > 1:915576/1‑60 (MQ=255) atGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:336096/1‑71 (MQ=255) accttgacctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttggt < 1:1363076/71‑1 (MQ=255) accttgacctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttggt < 1:643700/71‑1 (MQ=255) accttgacctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg > 1:2566667/1‑70 (MQ=255) ttgacctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:2768917/1‑64 (MQ=255) tgacctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAgcg > 1:2585346/1‑71 (MQ=255) acctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAGTCTGGTTGGTAGcgcg > 1:3745522/1‑71 (MQ=255) acctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGcgcg > 1:2486817/1‑71 (MQ=255) acctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGcgcg > 1:1797508/1‑71 (MQ=255) cctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGt < 1:2749202/71‑1 (MQ=255) ctGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGtt < 1:2688517/71‑1 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACa < 1:2271168/71‑1 (MQ=255) tACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg < 1:561261/70‑1 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttggt < 1:4694505/55‑1 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAc > 1:1993466/1‑71 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAc > 1:1765588/1‑71 (MQ=255) tGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGcgcg < 1:4546431/60‑1 (MQ=255) tGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGcgcg < 1:2675185/60‑1 (MQ=255) cTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcgcg > 1:2409512/1‑71 (MQ=255) cTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcgcg > 1:4343231/1‑71 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt < 1:2289671/69‑1 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt < 1:3690780/69‑1 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt < 1:4412154/69‑1 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTaa > 1:774416/1‑71 (MQ=255) aCCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATg < 1:943075/71‑1 (MQ=255) cGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAcgcg < 1:4016340/64‑1 (MQ=255) cGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCt < 1:3618391/71‑1 (MQ=255) cGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCt < 1:3284066/71‑1 (MQ=255) gCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTg > 1:2637173/1‑71 (MQ=255) aaGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTa < 1:3698224/71‑1 (MQ=255) gagGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTaa > 1:2655652/1‑61 (MQ=255) gagGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTaa > 1:1996479/1‑61 (MQ=255) gagGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTaa > 1:2126021/1‑61 (MQ=255) agGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAga > 1:4385545/1‑70 (MQ=255) agGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAga > 1:1731366/1‑70 (MQ=255) agGCAAAAGACCACGGTGAAAGCGGCAATCTGGTTGGTAGcgcg < 1:5142899/44‑1 (MQ=255) aaaGACCACGGTGAAGGCGGCAATCTGGTTGGTAgcgc < 1:265350/38‑1 (MQ=255) aaaGACCACGGTGAAGGCGGCAATCTGGTTGGTAgcgc < 1:859257/38‑1 (MQ=255) aaaGACCACGGTGAAGGCGGCAATCTGGTTGGTAGcgcg < 1:4233199/39‑1 (MQ=255) aaaGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGAtgt < 1:1552793/71‑1 (MQ=255) aaaGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGAtgt < 1:2177622/71‑1 (MQ=255) aaGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACa < 1:2681195/43‑1 (MQ=255) aaGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAgatg > 1:1769497/1‑66 (MQ=255) aaGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGAtgtg < 1:1539715/71‑1 (MQ=255) aaGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGAtgtg < 1:1398871/71‑1 (MQ=255) aaGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGAtgt > 1:3557480/1‑70 (MQ=255) aGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAgatga > 1:2305477/1‑66 (MQ=255) aGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAgatga > 1:5034266/1‑66 (MQ=255) gACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGAt < 1:2772328/71‑1 (MQ=255) aCCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAgatga < 1:1309175/64‑1 (MQ=255) aCCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAgatga < 1:1834799/64‑1 (MQ=255) aCCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATc < 1:4352945/71‑1 (MQ=255) aCCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATc < 1:3069458/71‑1 (MQ=255) aCCACGGTGAAAGCGGCTATCTGGTTGGTAGcgcg < 1:1874566/35‑1 (MQ=37) ccACGGTGAAGGCGGCAATCTGGTTGTTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGAt < 1:4765314/69‑1 (MQ=255) ccACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGAt < 1:3199891/69‑1 (MQ=255) ccACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGAt < 1:401400/69‑1 (MQ=255) ccACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATc < 1:1648333/70‑1 (MQ=255) ccACGGTGAAGACGGCAATCTGGTTGTTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGAtgt < 1:112293/66‑1 (MQ=255) cACGGTGAAGGCGGCAATCTTGTTGGTAGCGCGTTACAAGGACGCGt < 1:2309250/47‑1 (MQ=255) cACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt < 1:2598608/47‑1 (MQ=255) cACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt < 1:3333692/47‑1 (MQ=255) cACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTa < 1:151528/57‑1 (MQ=255) cACGGTGAAAGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAga > 1:3334072/1‑59 (MQ=255) cACGGTGAAAGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAga > 1:277384/1‑59 (MQ=255) cACGGTGAAAGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAga > 1:3337400/1‑59 (MQ=255) cGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAga > 1:755383/1‑57 (MQ=255) cGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAga > 1:763610/1‑57 (MQ=255) cGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAga > 1:1346927/1‑57 (MQ=255) gTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg < 1:931702/36‑1 (MQ=255) gTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg < 1:922670/36‑1 (MQ=255) gTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg < 1:4445191/36‑1 (MQ=255) gTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCAccgcc > 1:5024864/1‑71 (MQ=255) gTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCAccgcc > 1:2832139/1‑71 (MQ=255) gAAGGCGGCAATCTGGTTGGTAGCGCGTTGCAAGGACGCGTAATGCTGGTAGATGATGTGATCAccgccg > 1:3808536/1‑70 (MQ=255) gAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTa > 1:1180460/1‑42 (MQ=255) gAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCAccgccg > 1:1877665/1‑70 (MQ=255) gAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCAccgccg > 1:4436046/1‑70 (MQ=255) aaGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt < 1:28413/40‑1 (MQ=255) aaGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCgg > 1:83288/1‑70 (MQ=255) | CTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGG > W3110S.gb/3824901‑3825037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |