| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 285,498 | G→A | 26.3% | A294T (GCC→ACC) | yagG → | predicted sugar transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 285,498 | 0 | G | A | 26.3% | 102.1 / 36.2 | 57 | A294T (GCC→ACC) | yagG | predicted sugar transporter |
| Reads supporting (aligned to +/- strand): ref base G (18/24); new base A (7/8); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACA > W3110S.gb/285435‑285567 | cTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTc > 1:833091/1‑69 (MQ=255)cTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTc > 1:2680246/1‑69 (MQ=255)cTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTc > 1:178871/1‑69 (MQ=255)cTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCaa < 1:4335454/71‑1 (MQ=255) tCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCaca > 1:993304/1‑69 (MQ=255) gCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTg > 1:4460845/1‑71 (MQ=255) ccaTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatc < 1:2003680/71‑1 (MQ=255) aTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatcat > 1:1138644/1‑71 (MQ=255) gTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCa < 1:3467335/59‑1 (MQ=255) gTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCa < 1:773251/59‑1 (MQ=255) gTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCa < 1:1537194/59‑1 (MQ=255) ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatc < 1:105141/66‑1 (MQ=255) cGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatcat > 1:4224329/1‑66 (MQ=255) cGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCAtcgtcg > 1:1756161/1‑71 (MQ=255) cTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGcc < 1:4341407/70‑1 (MQ=255) cTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGcc < 1:2255818/70‑1 (MQ=255) cTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGcc < 1:4376812/70‑1 (MQ=255) tCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTa > 1:824693/1‑71 (MQ=255) tCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTa > 1:2392783/1‑71 (MQ=255) tCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa > 1:2162760/1‑71 (MQ=255) tCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa > 1:2868838/1‑71 (MQ=255) cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTAc > 1:4666386/1‑71 (MQ=255) ctcctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGa > 1:1939421/1‑71 (MQ=255) ctcctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGa > 1:2581657/1‑71 (MQ=255) ctcctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGa > 1:1545231/1‑71 (MQ=255) ctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatc < 1:3036208/49‑1 (MQ=255) ctcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatc < 1:167532/49‑1 (MQ=255) tcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCt < 1:271852/65‑1 (MQ=255) tcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCt < 1:4585183/65‑1 (MQ=255) tcACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCt < 1:3489195/65‑1 (MQ=255) aCGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGcc > 1:2141188/1‑71 (MQ=255) ccTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCctg > 1:2074376/1‑70 (MQ=255) ctgctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCctgct < 1:1855325/71‑1 (MQ=255) tgctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCAtcgtcg < 1:2393604/48‑1 (MQ=255) ctgGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCctgctg < 1:86136/69‑1 (MQ=255) cGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGt < 1:2507144/71‑1 (MQ=255) cGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGt < 1:1328483/71‑1 (MQ=255) gCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:130353/71‑1 (MQ=255) ttCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAc > 1:1831408/1‑71 (MQ=255) cGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAccc > 1:3890269/1‑71 (MQ=255) cGACCGCGTCACCGCCTTCAAGTGGATCATCATCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAccc > 1:3500931/1‑71 (MQ=255) cGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAc < 1:3751216/69‑1 (MQ=255) cgTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:4359065/71‑1 (MQ=255) cgTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:1309031/71‑1 (MQ=255) cgTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:1395375/71‑1 (MQ=255) cgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGt > 1:4892621/1‑60 (MQ=255) cgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:3034220/71‑1 (MQ=255) cgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:2549957/71‑1 (MQ=255) cgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:3547618/71‑1 (MQ=255) cgTCACCACATTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcgg < 1:906323/71‑1 (MQ=255) tCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:4703878/59‑1 (MQ=255) tCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:3109041/59‑1 (MQ=255) tCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:490771/59‑1 (MQ=255) tCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTc < 1:2254993/59‑1 (MQ=255) accaccTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTc > 1:3366745/1‑54 (MQ=255) aCCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGca > 1:4857480/1‑71 (MQ=255) aCCGCCTTAAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGca > 1:1725340/1‑71 (MQ=255) cGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGcaca > 1:3139943/1‑71 (MQ=255) | CTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACA > W3110S.gb/285435‑285567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |