| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,849,808 | T→C | 8.9% | F246S (TTT→TCT) | tdh → | threonine 3‑dehydrogenase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,849,808 | 0 | T | C | 8.9% | 151.2 / 7.0 | 56 | F246S (TTT→TCT) | tdh | threonine 3‑dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base C (3/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
ATGGCGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTATTCCGCCGTCTGATAT > W3110S.gb/3849741‑3849877 | aTGGCGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGtctc < 1:4095624/70‑1 (MQ=255)aTGGCGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCg < 1:3186332/71‑1 (MQ=255) tGGCGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGt < 1:3615277/71‑1 (MQ=255) tGGCGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGt < 1:3507966/71‑1 (MQ=255) ggCGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGTCAGCGTCTCGTa > 1:2544456/1‑71 (MQ=255) ggCGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTCTCGTa > 1:3189399/1‑71 (MQ=255) cGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTAcc < 1:3891598/71‑1 (MQ=255) aGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCtt > 1:5042896/1‑70 (MQ=255) aGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCtt < 1:4396890/70‑1 (MQ=255) aGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCtt < 1:1551377/70‑1 (MQ=255) aTGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTCTCGTACCATGCTTGacac > 1:3964935/1‑71 (MQ=255) aCCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCg > 1:3353140/1‑53 (MQ=255) aCCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCAt < 1:165686/71‑1 (MQ=255) cGAAGGTTTTGATGTCGGTC‑‑GAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAAt < 1:1080299/71‑1 (MQ=255) aGGTTTTGATGTCTGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAAt < 1:5115105/70‑1 (MQ=255) aGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGa > 1:3143755/1‑60 (MQ=255) tttGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGa > 1:2306132/1‑64 (MQ=255) tttGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCAcgg > 1:3901881/1‑71 (MQ=255) tttGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCAcgg > 1:2925111/1‑71 (MQ=255) tGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCAcggcg > 1:1864208/1‑71 (MQ=255) tGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACggcggc < 1:1455957/70‑1 (MQ=255) gTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCg > 1:4065286/1‑71 (MQ=255) tCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCg < 1:1743770/70‑1 (MQ=255) ggTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTAt < 1:4489794/71‑1 (MQ=255) ggTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGACGTAt < 1:2944690/71‑1 (MQ=255) tCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTg > 1:1347684/1‑71 (MQ=255) cTGGAAATGTCCAGTGTGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTg < 1:3798482/70‑1 (MQ=255) tGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCtt < 1:1434568/41‑1 (MQ=255) tGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCtt < 1:102162/41‑1 (MQ=255) tGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCtt < 1:1688681/41‑1 (MQ=255) gAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTa > 1:3917480/1‑64 (MQ=255) gAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGc < 1:4484088/68‑1 (MQ=255) gAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGc < 1:1147470/68‑1 (MQ=255) gAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGa > 1:3754306/1‑70 (MQ=255) gAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGAt > 1:3337657/1‑71 (MQ=255) gAAATGTCCGGTGCGCCGCCAGCGTCTCGTACCATGCTTGACACCATTAATCACGGCGGCCGTATTGCGa < 1:4630618/70‑1 (MQ=255) gAAATGTCCGGTGCGCCGCCAGCGTCTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGa < 1:2959398/70‑1 (MQ=255) aaaTGTCCGGTTCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGc < 1:2443793/67‑1 (MQ=255) aaaTGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAAt < 1:2859501/50‑1 (MQ=255) aaaTGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAAt < 1:4737420/50‑1 (MQ=255) aaaTGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAAt < 1:2789394/50‑1 (MQ=255) aTGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCTATGCt > 1:652581/1‑71 (MQ=255) tGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTg > 1:2095333/1‑71 (MQ=255) tCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCa > 1:1483809/1‑47 (MQ=255) cGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTa > 1:193124/1‑71 (MQ=255) cGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTa < 1:1686621/71‑1 (MQ=255) gTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTAtt > 1:4324391/1‑71 (MQ=255) gcgcCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTATTcc > 1:1677344/1‑71 (MQ=255) gcgcCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTATTcc > 1:1894304/1‑71 (MQ=255) gccAGCGTTTCGTACCATGCTTGCCACCATGAATCAcggcg > 1:3449087/1‑41 (MQ=255) gccAGCGTTTCGTACCATGCTTGACACCATGAATCAcggcg > 1:4275858/1‑41 (MQ=255) gCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTa > 1:2309283/1‑43 (MQ=255) ggTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTa > 1:3341332/2‑42 (MQ=255) cGTTTCGTACCATGCTTGACACCATGAATGACGGCGGCCGTa > 1:4577016/1‑42 (MQ=255) cGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTa > 1:535859/1‑42 (MQ=255) cGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGa > 1:4194497/1‑48 (MQ=255) gTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTATTCCGCCGTCTGata > 1:4790787/1‑71 (MQ=255) tttCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTATTCCGCCGTCTGatat < 1:3628236/71‑1 (MQ=255) ttCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGa > 1:4124684/1‑45 (MQ=255) ttCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTa > 1:2145430/1‑54 (MQ=255) ttCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTa > 1:2029385/1‑54 (MQ=255) | ATGGCGGAGTTAGGCATGACCGAAGGTTTTGATGTCGGTCTGGAAATGTCCGGTGCGCCGCCAGCGTTTCGTACCATGCTTGACACCATGAATCACGGCGGCCGTATTGCGATGCTGGGTATTCCGCCGTCTGATAT > W3110S.gb/3849741‑3849877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |