| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,915,008 | C→T | 5.5% | intergenic (+4/‑91) | ysaB → / → glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,915,008 | 0 | C | T | 5.5% | 226.9 / 2.3 | 72 | intergenic (+4/‑91) | ysaB/glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (38/30); new base T (2/2); total (40/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGTAAAGAGCAGTTTGTCTGTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAAT > W3110S.gb/3914938‑3915073 | ccGTAAAGAGCAGTTTGTCTGTTCTTTTGACGCGGATGGCCATTTTTTGAATCTTTCCATGAGTTAAGccc > 1:3865007/1‑71 (MQ=255) aaaGAGCAGTTTGTCTGTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCt > 1:1246386/1‑71 (MQ=255) tttGTCTGTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCttt < 1:431435/64‑1 (MQ=255) tGTCTGTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGttt > 1:2383654/1‑71 (MQ=255) gTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGccc > 1:3827324/1‑51 (MQ=255) gTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGccc > 1:4152902/1‑51 (MQ=255) gTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCg < 1:3741464/68‑1 (MQ=255) gTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCg < 1:4047400/68‑1 (MQ=255) ttCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCg < 1:4697811/62‑1 (MQ=255) ttCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACt > 1:2353826/1‑71 (MQ=255) tCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg > 1:3362693/1‑71 (MQ=255) tCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg > 1:1769748/1‑71 (MQ=255) cTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACtc > 1:1002231/1‑69 (MQ=255) cTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGtt > 1:2523150/1‑62 (MQ=255) cTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:1386110/70‑1 (MQ=255) ttttGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCg < 1:3263456/64‑1 (MQ=255) ttttGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGt < 1:2105937/65‑1 (MQ=255) tttGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCg > 1:3549667/1‑58 (MQ=255) tGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtaca < 1:4550274/70‑3 (MQ=255) aCGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATc < 1:3023552/70‑1 (MQ=255) aCGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCt < 1:2209655/71‑1 (MQ=255) gcgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtc < 1:4036000/71‑1 (MQ=255) ggATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCg < 1:1630365/55‑1 (MQ=255) gATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:239041/59‑1 (MQ=255) tGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGta > 1:3884084/1‑59 (MQ=255) tGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGta > 1:639022/1‑59 (MQ=255) gCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTa > 1:1673332/1‑52 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:2642391/1‑71 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:4096296/1‑71 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:3314985/1‑71 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGt > 1:3620137/1‑47 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccat < 1:2372657/63‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccat < 1:1754150/63‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccat < 1:4630467/63‑1 (MQ=255) tttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGt > 1:1207244/1‑41 (MQ=255) tttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccatcca > 1:3578575/1‑65 (MQ=255) ttttGCATGTTTCCATGCGTTAAGCTCTGCTTTTTCCCGTTTCGTACTGTacat > 1:1431297/1‑54 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCttttt > 1:1255030/1‑35 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTcc > 1:4988402/1‑37 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg > 1:2110112/1‑49 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg > 1:2984810/1‑49 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTacat > 1:2875569/1‑54 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:1787487/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATc > 1:2667539/1‑55 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta > 1:2996543/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta > 1:1864590/1‑71 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:1521399/70‑1 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:2311354/1‑70 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:3941380/1‑70 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:3438760/70‑1 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:5038911/1‑70 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta < 1:2716401/70‑1 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta < 1:1794922/70‑1 (MQ=255) gCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATAc > 1:718155/1‑70 (MQ=255) cATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCg < 1:1044061/39‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:3356143/43‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:1953873/43‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTg < 1:500268/43‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGtata < 1:1029262/67‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGtata < 1:4919944/67‑1 (MQ=255) cTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCggg < 1:653318/61‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:2695137/57‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:2289176/57‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:1378241/57‑1 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGta > 1:3867598/1‑42 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACt > 1:2865101/1‑66 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCtt > 1:1395177/1‑47 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCg > 1:2240750/1‑57 (MQ=255) tCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTAGTGTATATCTTCCATCCAGCGGGTATACTGATccc > 1:2125753/1‑70 (MQ=255) tGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGt < 1:1967270/35‑1 (MQ=255) cGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtac > 1:2614077/1‑34 (MQ=255) cGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtac > 1:3790136/1‑34 (MQ=255) gTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa < 1:1947958/71‑1 (MQ=255) aaGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:3182458/1‑52 (MQ=255) aaGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAAt > 1:1718781/1‑71 (MQ=255) cTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:3475198/45‑1 (MQ=255) | CCGTAAAGAGCAGTTTGTCTGTTCTTTTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAAT > W3110S.gb/3914938‑3915073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |