| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,915,033 | T→C | 31.2% | intergenic (+29/‑66) | ysaB → / → glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,915,033 | 0 | T | C | 31.2% | 82.7 / 55.3 | 64 | intergenic (+29/‑66) | ysaB/glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (20/24); new base C (8/12); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATGCA > W3110S.gb/3914967‑3915103 | aCGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATc < 1:3023552/70‑1 (MQ=255)aCGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCt < 1:2209655/71‑1 (MQ=255) gcgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtc < 1:4036000/71‑1 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:3314985/1‑71 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:4096296/1‑71 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:2642391/1‑71 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccat < 1:4630467/63‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccat < 1:1754150/63‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccat < 1:2372657/63‑1 (MQ=255) tttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccatcca > 1:3578575/1‑65 (MQ=255) ttttGCATGTTTCCATGCGTTAAGCTCTGCTTTTTCCCGTTTCGTACTGTacat > 1:1431297/1‑54 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTacat > 1:2875569/1‑54 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:1787487/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATc > 1:2667539/1‑55 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta > 1:1864590/1‑71 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta > 1:2996543/1‑71 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:3941380/1‑70 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:3438760/70‑1 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:5038911/1‑70 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:1521399/70‑1 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:2311354/1‑70 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta < 1:2716401/70‑1 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGta < 1:1794922/70‑1 (MQ=255) gCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATAc > 1:718155/1‑70 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGtata < 1:4919944/67‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGtata < 1:1029262/67‑1 (MQ=255) cTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCggg < 1:653318/61‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:1378241/57‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:2695137/57‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:2289176/57‑1 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACt > 1:2865101/1‑66 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCtt > 1:1395177/1‑47 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCg > 1:2240750/1‑57 (MQ=255) tCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTAGTGTATATCTTCCATCCAGCGGGTATACTGATccc > 1:2125753/1‑70 (MQ=255) gTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa < 1:1947958/71‑1 (MQ=255) aaGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:3182458/1‑52 (MQ=255) aaGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAAt > 1:1718781/1‑71 (MQ=255) cTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:3475198/45‑1 (MQ=255) tGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa < 1:3824334/62‑1 (MQ=255) gCTTTTTCCCGTTTCGTATTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCca > 1:3520034/1‑67 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:3583325/38‑1 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:391563/38‑1 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:639576/38‑1 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:701590/38‑1 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:1897285/38‑1 (MQ=255) cTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTaa > 1:3737020/1‑61 (MQ=255) cTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACg > 1:3660285/1‑70 (MQ=255) cTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGtata > 1:1259356/1‑44 (MQ=255) tttttCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:794174/1‑38 (MQ=255) tttttCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:2491649/1‑38 (MQ=255) tttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATc > 1:2880616/1‑71 (MQ=255) ttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCttt < 1:2841194/55‑1 (MQ=255) ttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCttt < 1:4771460/55‑1 (MQ=255) ttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCcaca > 1:4013416/1‑64 (MQ=255) tCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATc > 1:4357238/1‑69 (MQ=255) ccGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAg < 1:195782/70‑1 (MQ=255) ccGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAg < 1:4744929/70‑1 (MQ=255) ccGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAg < 1:2925350/70‑1 (MQ=255) ccGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAg < 1:763311/70‑1 (MQ=255) ccGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAg < 1:776746/70‑1 (MQ=255) cGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAg < 1:2756310/69‑1 (MQ=255) gTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACTCGTATCCAg < 1:3581452/68‑1 (MQ=255) tttCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATc < 1:4814218/64‑1 (MQ=255) tttCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATc < 1:4541050/64‑1 (MQ=255) tttCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCAc > 1:3622412/1‑70 (MQ=255) tGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCttt < 1:3225827/41‑1 (MQ=255) tataTCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTaa > 1:2548691/1‑41 (MQ=255) atatCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATg > 1:4939881/1‑70 (MQ=255) tatCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATGCa < 1:32681/71‑1 (MQ=255) tatCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATGCa < 1:676293/71‑1 (MQ=255) | ACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATGCA > W3110S.gb/3914967‑3915103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |