| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,915,241 | A→G | 30.2% | E48G (GAG→GGG) | glyQ → | glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,915,241 | 0 | A | G | 30.2% | 53.6 / 33.3 | 43 | E48G (GAG→GGG) | glyQ | glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (18/12); new base G (5/8); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
TTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTACG > W3110S.gb/3915178‑3915309 | ttGTTCAACCATTGGACATGGTAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTgggg > 1:3201253/1‑71 (MQ=255)ttGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTgg < 1:73552/69‑1 (MQ=255) gTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGc > 1:3786094/1‑70 (MQ=255) ccATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAAcc < 1:3790545/71‑1 (MQ=255) ttGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGAt > 1:4523004/1‑71 (MQ=255) ttGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGAt < 1:3808148/71‑1 (MQ=255) ttGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGAt < 1:2913504/71‑1 (MQ=255) ttGGACACGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGAt < 1:5014180/71‑1 (MQ=255) aCATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGa < 1:4756912/66‑1 (MQ=255) ggAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGgctg > 1:544761/1‑71 (MQ=255) ggAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGgctg > 1:4171129/1‑71 (MQ=255) aaGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgctgct < 1:2332488/71‑1 (MQ=255) gTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGc < 1:1670094/43‑1 (MQ=255) tCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAAcc < 1:2777322/55‑1 (MQ=255) tCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAAcc < 1:2436518/55‑1 (MQ=255) ggCGCGGGAACCTCTCACCCAATGACCTGTCTGCGGGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATg < 1:2722932/70‑1 (MQ=255) ggCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATg < 1:636914/70‑1 (MQ=255) ggCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATg < 1:2252079/70‑1 (MQ=255) gcgGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCa < 1:4455536/71‑1 (MQ=255) gcgGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCa < 1:2942134/71‑1 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCt > 1:2809654/1‑71 (MQ=255) ctctCACCCAATGACCTGTCTTCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTtctc < 1:1346710/71‑1 (MQ=255) ctctCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATg < 1:4388981/59‑1 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgctg > 1:2927088/1‑51 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgctg > 1:1103514/1‑51 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgctg > 1:226846/1‑51 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgctg > 1:4940333/1‑51 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGgctg > 1:3090733/1‑51 (MQ=255) ctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcgt > 1:3284374/1‑71 (MQ=255) ccAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGccc > 1:4725686/1‑71 (MQ=255) ccAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGccc > 1:4119435/1‑71 (MQ=255) ccAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGccc > 1:2892561/1‑71 (MQ=255) aTGACCTGTCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGc > 1:2979030/1‑55 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTtctc < 1:3566218/61‑1 (MQ=255) aTGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgac > 1:3618838/1‑71 (MQ=255) gACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcgt > 1:2982550/1‑61 (MQ=255) tCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTAt < 1:2847011/40‑1 (MQ=255) tCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTc > 1:2417448/1‑71 (MQ=255) tGCTCGATCTGGCGCCAGAACCGATGGCGGCTGCTTATGTTCAGcc > 1:2347602/1‑46 (MQ=38) tGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGc > 1:3046975/1‑71 (MQ=255) cgcgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGc < 1:2718205/69‑1 (MQ=255) cgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTACg > 1:3672868/1‑71 (MQ=255) cgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTACg > 1:3166786/1‑71 (MQ=255) | TTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTACG > W3110S.gb/3915178‑3915309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |