| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,916,520 | G→A | 13.0% | L167L (CTG→CTA) | glyS → | glycine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,916,520 | 0 | G | A | 13.0% | 192.9 / 17.1 | 69 | L167L (CTG→CTA) | glyS | glycine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (35/25); new base A (4/5); total (39/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CCCGAAACTGATGCGTTGGGGCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTCGCGGCCAC > W3110S.gb/3916451‑3916586 | cccGAAACTGATGCGTTGGGGCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCctgctgc > 1:2773211/1‑71 (MQ=255)cccGAAACTGATGCGTTGGGGCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCctgctgc > 1:4566255/1‑71 (MQ=255) aaaCTGATGCGTTGGGGCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTggg > 1:2739154/1‑71 (MQ=255) aTGCGTTGGGGCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACa < 1:152531/70‑1 (MQ=255) gCGTTGGGGCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAg > 1:2471963/1‑71 (MQ=255) ggggCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACaaa > 1:1237780/1‑65 (MQ=255) ggggCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCAtt > 1:2961648/1‑71 (MQ=255) ggggCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCAt < 1:1762735/70‑1 (MQ=255) tgcgcAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTACTGGGCGAc < 1:1830664/59‑1 (MQ=255) cgcAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTACTGGGCGAc < 1:626433/58‑1 (MQ=255) cAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGc < 1:3490062/70‑1 (MQ=255) cGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCa < 1:4112423/71‑1 (MQ=255) aCGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCa > 1:3717241/1‑69 (MQ=255) aCGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCa > 1:4972962/1‑69 (MQ=255) aCGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTACTGGGCGACAAAGTCATTc > 1:745834/1‑60 (MQ=255) cGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCaa > 1:4735629/1‑65 (MQ=255) cGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCaa > 1:4430605/1‑65 (MQ=255) cGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTc > 1:1425750/1‑71 (MQ=255) gTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCt < 1:1400183/71‑1 (MQ=255) cTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCa > 1:3027234/1‑71 (MQ=255) tCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGc > 1:1433432/1‑55 (MQ=255) cGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGt > 1:1331352/1‑45 (MQ=255) cGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCAtt > 1:3286856/1‑61 (MQ=255) cGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTc > 1:1884938/1‑62 (MQ=255) cGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt < 1:4149379/70‑1 (MQ=255) cGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt > 1:2200457/1‑70 (MQ=255) cGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt > 1:535213/1‑70 (MQ=255) cGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt > 1:1342082/1‑70 (MQ=255) cGTGCGTCCGGTGCACACCGTGACCCTGATGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt > 1:1733812/1‑70 (MQ=255) cGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTc > 1:5028450/1‑71 (MQ=255) cGTCCGGTGCACACCGTGACCCTGCTACTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTc > 1:595474/1‑71 (MQ=255) tCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCg < 1:230334/71‑1 (MQ=255) gTGCAGACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATcg > 1:4719999/1‑71 (MQ=255) gTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTcc < 1:4810122/66‑1 (MQ=255) gTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATcg < 1:4148725/71‑1 (MQ=255) tGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATcgc > 1:4833609/1‑71 (MQ=255) gCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCt > 1:2947822/1‑51 (MQ=255) cacaCCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGTCATTCAGTCCGATCGCGt > 1:4128965/1‑71 (MQ=255) cacaCCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGt > 1:3150765/1‑71 (MQ=255) cacaCCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGt > 1:2121585/1‑71 (MQ=255) cacaCCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGt < 1:173891/71‑1 (MQ=255) cacaCCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGt > 1:3724285/1‑71 (MQ=255) cacaCCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGt > 1:1679076/1‑71 (MQ=255) cacaCCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGt > 1:1053774/1‑71 (MQ=255) cacCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTc < 1:444706/47‑1 (MQ=255) cacCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTc < 1:1403617/47‑1 (MQ=255) cacCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt < 1:399074/55‑1 (MQ=255) cacCGTGACCCTGCTACTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGa > 1:2587783/1‑71 (MQ=255) cacCGTGACCCTGCTACTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGa > 1:4160849/1‑71 (MQ=255) acCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCaa < 1:1132211/40‑1 (MQ=255) acCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGa > 1:659358/1‑70 (MQ=255) acCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGAt < 1:5003393/71‑1 (MQ=255) acCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGAt > 1:2885301/1‑71 (MQ=255) ccGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGc < 1:1712415/37‑1 (MQ=255) ccGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTc < 1:4658925/45‑1 (MQ=255) ccGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGt > 1:4216481/1‑67 (MQ=255) ccGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGAtt > 1:3857934/1‑71 (MQ=255) ccGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGAtt > 1:279285/1‑71 (MQ=255) cGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGAt > 1:3351620/1‑61 (MQ=255) cGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTc < 1:896371/71‑1 (MQ=255) gTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCAta < 1:578113/42‑2 (MQ=255) gTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTcg < 1:4437089/71‑1 (MQ=255) gTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTcg < 1:2081639/71‑1 (MQ=255) gTGACCCTGCTACTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCTATCGCGTGATTcg < 1:367815/71‑1 (MQ=255) gTGACCCTGCTACTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTcg < 1:1482956/71‑1 (MQ=255) gTGACCCTGCTACTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTcg < 1:269605/71‑1 (MQ=255) tGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt < 1:3984341/50‑1 (MQ=255) tGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt < 1:5141139/50‑1 (MQ=255) tGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAtt < 1:2526618/50‑1 (MQ=255) tGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTcgc > 1:946993/1‑71 (MQ=255) aCCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTc < 1:4774849/53‑1 (MQ=255) tgctgctgGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAt < 1:4070518/43‑1 (MQ=255) tgctgctgGGCGACAAAGTCATTCCGGCAACCATTCTGGGCAt < 1:929364/43‑1 (MQ=255) tgctgctgGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGAt > 1:2400573/1‑61 (MQ=255) tgctgctgGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTCGCGGCCAc > 1:1295377/1‑71 (MQ=255) gctgctgGGCGACAAAGTCATTCCGGCAACCATTc > 1:846201/1‑35 (MQ=255) gctgcagGGCGACAAACTCATTCCGGCAACCATTCTGGGCATTCa > 1:381297/1‑45 (MQ=38) | CCCGAAACTGATGCGTTGGGGCGCAAGCGACGTGCACTTCGTGCGTCCGGTGCACACCGTGACCCTGCTGCTGGGCGACAAAGTCATTCCGGCAACCATTCTGGGCATTCAGTCCGATCGCGTGATTCGCGGCCAC > W3110S.gb/3916451‑3916586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |