Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,936,857 G→A 9.4% D97N (GAC→AAC)  dppD → dipeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,936,8570GA 9.4% 162.2 / 6.2 53D97N (GAC→AAC) dppDdipeptide transporter
Reads supporting (aligned to +/- strand):  ref base G (25/23);  new base A (3/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

AGCGTATCTCAGAAAAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCAT  >  W3110S.gb/3936795‑3936925
                                                              |                                                                    
aGCGTATCTCAGAAAAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATg                                                              <  1:1569870/71‑1 (MQ=255)
        tcAGAAAAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGAt                                                               <  1:692984/62‑1 (MQ=255)
         cAGAAAAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCt                                                      >  1:879124/1‑70 (MQ=255)
           gAAAAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGa                                                             <  1:3738929/61‑1 (MQ=255)
           gAAAAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTaa                                                   <  1:3163335/71‑1 (MQ=255)
                      ccgcAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTaca                                        >  1:440030/1‑71 (MQ=255)
                         cAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCg                                     <  1:3084895/71‑1 (MQ=255)
                            ccTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCg                                                                 <  1:894687/40‑1 (MQ=255)
                            ccTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTgg                                  >  1:1302258/1‑71 (MQ=255)
                            ccTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTgg                                  >  1:484525/1‑71 (MQ=255)
                            ccTGGTGGGTGCCGAAGTGCCGATGATCTTCCAGGACCCg                                                                 <  1:4960663/40‑1 (MQ=255)
                              tggtggGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGc                                            <  1:2206137/59‑1 (MQ=255)
                              tggtggGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGt                                >  1:838732/1‑71 (MQ=255)
                              tggtggGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGt                                >  1:1308623/1‑71 (MQ=255)
                              tggtggGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGt                                >  1:463051/1‑71 (MQ=255)
                              tggtggGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGt                                >  1:1306227/1‑71 (MQ=255)
                               ggtgggttCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGc                                            <  1:4767652/58‑1 (MQ=255)
                               ggtgggtgCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGtt                               >  1:1413380/1‑71 (MQ=255)
                                      gCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGAt                        >  1:1923525/1‑71 (MQ=255)
                                       ccGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGAt                        <  1:4549400/70‑1 (MQ=255)
                                       ccGAAGTGGCGATGATCTTCCAGAACCCGATGACCAGCCTTAACCCGTGc                                            >  1:4209323/1‑50 (MQ=255)
                                       ccGAAGTGGCGATGATCTTCCAGAACCCGATGACCAGCCTTAACCCGTGc                                            >  1:4628751/1‑50 (MQ=255)
                                       ccGAAGTGGCGATGATCTTCCAGAACCCGATGACCAGCCTTAACCCGTGc                                            >  1:4622857/1‑50 (MQ=255)
                                           aGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCTTGGGTTTCCAGATTATg                    >  1:3948579/1‑70 (MQ=255)
                                           aGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATg                    >  1:407165/1‑70 (MQ=255)
                                           aGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCCTGGGTTTCCAGATTATg                    >  1:160159/1‑70 (MQ=255)
                                            gTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGAt                        <  1:3956713/65‑1 (MQ=255)
                                               gCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGa                  >  1:4792903/1‑68 (MQ=255)
                                               gCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGa                  >  1:1996829/1‑68 (MQ=255)
                                               gCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGc               >  1:4479849/1‑71 (MQ=255)
                                               gCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGc               <  1:1551808/71‑1 (MQ=255)
                                                  atgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATgg                   <  1:2070314/64‑1 (MQ=255)
                                                  atgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATgg                   <  1:3457342/64‑1 (MQ=255)
                                                  atgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAt            <  1:1202654/71‑1 (MQ=255)
                                                  atgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAt            <  1:3094505/71‑1 (MQ=255)
                                                   tgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTgg                                  <  1:2637660/48‑1 (MQ=255)
                                                   tgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTgg                                  <  1:3935141/48‑1 (MQ=255)
                                                   tgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTgg                                  <  1:2117597/48‑1 (MQ=255)
                                                   tgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAt            >  1:2993336/1‑70 (MQ=255)
                                                   tgatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAt            >  1:1617271/1‑70 (MQ=255)
                                                   tgatCTTCCAGAACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAtt           <  1:1916798/71‑1 (MQ=255)
                                                   tgatCTTCCAGAACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAtt           <  1:4930983/71‑1 (MQ=255)
                                                    gatCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCa                           <  1:4670619/54‑1 (MQ=255)
                                                     atCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAt            >  1:1530939/1‑68 (MQ=255)
                                                     atCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAt            >  1:3242391/1‑68 (MQ=255)
                                                     atCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGATTa          <  1:1164163/70‑1 (MQ=255)
                                                      tCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGATTAAg        >  1:3172401/1‑71 (MQ=255)
                                                        ttCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGa                  >  1:2155039/1‑59 (MQ=255)
                                                        ttCCAGGACCAGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAATAAGGt      >  1:425859/1‑71 (MQ=255)
                                                         tCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGATTAAGGTg     >  1:1392393/1‑71 (MQ=255)
                                                           cAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCa   >  1:5062397/1‑71 (MQ=255)
                                                            aGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGAtt           <  1:4356676/62‑1 (MQ=255)
                                                            aGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGATTaa         <  1:486695/64‑1 (MQ=255)
                                                             ggACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCAt  >  1:1416906/1‑70 (MQ=255)
                                                              gACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATg                    <  1:2969413/51‑1 (MQ=255)
                                                              |                                                                    
AGCGTATCTCAGAAAAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACACCGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCAT  >  W3110S.gb/3936795‑3936925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑