| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,980,009 | T→C | 13.3% | intergenic (‑6/+19) | mdtF ← / ← mdtE | multidrug transporter, RpoS‑dependent/multidrug resistance efflux transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,980,009 | 0 | T | C | 13.3% | 152.2 / 15.7 | 60 | intergenic (‑6/+19) | mdtF/mdtE | multidrug transporter, RpoS‑dependent/multidrug resistance efflux transporter |
| Reads supporting (aligned to +/- strand): ref base T (28/24); new base C (2/6); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
CAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAATTGCT > W3110S.gb/3979940‑3980076 | cAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTc < 1:2150469/71‑1 (MQ=255) catAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCtt > 1:638965/1‑69 (MQ=255) catAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCtt > 1:4299707/1‑69 (MQ=255) catAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAAAAGTTAGCCATGAAAGTCCCCtt > 1:3238347/1‑69 (MQ=255) aataatGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTcc < 1:2995218/62‑1 (MQ=255) aatGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCt > 1:5072719/1‑69 (MQ=255) aatGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTg > 1:2473379/1‑70 (MQ=255) aatGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTg > 1:171441/1‑70 (MQ=255) atGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGc > 1:1839594/1‑70 (MQ=255) atGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGc < 1:740964/70‑1 (MQ=255) aCCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGCCCCCTTAAGCCTGCAACGTTATTg > 1:3217358/1‑71 (MQ=255) ccAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGtt < 1:3556482/71‑1 (MQ=255) cAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGCCCCCTTAAGCCTGCAACGTTATTGttt > 1:565950/1‑71 (MQ=255) aGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTc < 1:3433852/71‑1 (MQ=255) ggCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTAt > 1:1395111/1‑64 (MQ=255) gCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCtt < 1:3492306/47‑1 (MQ=255) gCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGc > 1:3803582/1‑51 (MQ=255) gCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTc < 1:2592681/69‑1 (MQ=255) gCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCg > 1:1166661/1‑70 (MQ=255) gCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGa < 1:4703051/71‑1 (MQ=255) aaaaaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGtt > 1:4451909/1‑59 (MQ=255) aaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTcccc > 1:4844986/1‑40 (MQ=255) aaCCGGGCGATCAATAAAATAGTTAGCCATGAAAGTcccc > 1:4677889/1‑40 (MQ=255) gCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGtt < 1:2793644/56‑1 (MQ=255) gCGATCAATAAAATAGTTAGCCATGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTgg < 1:5088410/71‑1 (MQ=255) gCGATCAATAAAATAGTTAGCCATGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTgg < 1:4621369/71‑1 (MQ=255) gCGATCAATAAAATAGTTAGCCAAGAAAGCCCCCTTAAGCCTGCAACGTTAtt < 1:827291/53‑1 (MQ=255) gATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCg < 1:3431137/71‑1 (MQ=255) aTCAATAAAATAGTTAGCCATGAAAGTCCCGTTAAGCCTGCAACGTTATTGTTTCGATTc < 1:959448/60‑1 (MQ=255) aTCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTAt > 1:251659/1‑49 (MQ=255) tCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCaa > 1:1853605/1‑42 (MQ=255) tCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTTTTTc < 1:1897090/54‑1 (MQ=255) tCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCgg < 1:601971/61‑1 (MQ=255) tCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGtt < 1:4982870/71‑1 (MQ=255) aaTAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGAt < 1:1141305/55‑1 (MQ=255) aaTAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGtttt < 1:2059436/71‑1 (MQ=255) tAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTAtt < 1:2226045/45‑1 (MQ=255) tAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTc > 1:759909/1‑70 (MQ=255) aaaaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCct > 1:535926/1‑71 (MQ=255) aaaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGc > 1:3253018/1‑58 (MQ=255) aaaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCctg > 1:4601634/1‑71 (MQ=255) aaTAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCctg < 1:361387/70‑1 (MQ=255) ttAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGttt < 1:4620623/60‑1 (MQ=255) tAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTggag > 1:2364640/1‑71 (MQ=255) aGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTg > 1:5048983/1‑36 (MQ=255) aGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTggag > 1:190346/1‑70 (MQ=255) gCCATGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTggagga < 1:4773598/71‑1 (MQ=255) cATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGaaa < 1:3677430/71‑1 (MQ=255) cATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGaaa < 1:3187295/71‑1 (MQ=255) aTGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCt > 1:367362/1‑47 (MQ=255) aTGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAAt > 1:4892828/1‑71 (MQ=255) tGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTc < 1:4452387/40‑1 (MQ=255) tGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGc < 1:2680473/45‑1 (MQ=255) tGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCctggctgg < 1:1763979/63‑1 (MQ=255) tGAAAGCCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAAtt < 1:4598603/71‑1 (MQ=255) gAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGtt > 1:2192719/1‑51 (MQ=255) gAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGtt > 1:2683411/1‑51 (MQ=255) aaaGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGt > 1:2979112/1‑41 (MQ=255) aaaGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTg > 1:2427484/1‑45 (MQ=255) aaGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAATTGCt < 1:4181544/71‑1 (MQ=255) tCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCctggctgg < 1:2185322/57‑1 (MQ=255) | CAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATAGTTAGCCATGAAAGTCCCCTTAAGCCTGCAACGTTATTGTTTCGATTCGGTGCTGGCGTTTTCCTGGCTGGAGGAAATTGCT > W3110S.gb/3979940‑3980076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |