| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,000,389 | A→G | 41.1% | intergenic (‑83/‑308) | uspA ← / → yhiO | universal stress global response regulator/predicted universal stress (ethanol tolerance) protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,000,389 | 0 | A | G | 41.1% | 36.9 / 91.4 | 73 | intergenic (‑83/‑308) | uspA/yhiO | universal stress global response regulator/predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (20/23); new base G (14/16); total (34/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.03e-01 | |||||||||||
CCATAAAGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGGAT > W3110S.gb/4000320‑4000458 | ccATAAAGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGATCTAc > 1:1497418/1‑71 (MQ=255) aaGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACtgcgtgcg < 1:2929586/71‑1 (MQ=255) aaGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACtgcgtgcg < 1:567772/71‑1 (MQ=255) gTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGcgcg > 1:2442494/1‑71 (MQ=255) gTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGcgcg > 1:1784857/1‑71 (MQ=255) gTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGcgcg > 1:1099724/1‑71 (MQ=255) gTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGcgcg > 1:810049/1‑71 (MQ=255) gTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGACACCCGCGTCCTGCGAACTACGTGcgcg < 1:632672/71‑1 (MQ=255) gATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTa < 1:823790/71‑1 (MQ=255) aTGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTaa < 1:815144/71‑1 (MQ=255) aTGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTg < 1:2697092/60‑1 (MQ=255) aTGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTg < 1:2269909/60‑1 (MQ=255) gACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAAcc > 1:2054704/1‑71 (MQ=255) aGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTa < 1:850216/71‑1 (MQ=255) gCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTgcgc < 1:3230885/52‑1 (MQ=255) gCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTaa < 1:1252287/71‑1 (MQ=255) cTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTaaa < 1:1895501/71‑1 (MQ=255) tAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTaaa < 1:1225854/70‑1 (MQ=255) aGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTaa > 1:588872/1‑68 (MQ=255) aGCGTTTCTTGTGCCCTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTa > 1:4215921/1‑67 (MQ=255) gCGTTTCTTGTGCCGTTACGTCACCGGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGa < 1:334140/70‑1 (MQ=255) gCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGa > 1:824186/1‑70 (MQ=255) gCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGAt > 1:2555631/1‑71 (MQ=255) tttCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGAtt < 1:2207582/69‑1 (MQ=255) ttCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTgcg < 1:616533/43‑1 (MQ=255) tCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTgcg > 1:1980183/1‑42 (MQ=255) tCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTaa > 1:2436928/1‑51 (MQ=255) tCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgt > 1:4079258/1‑71 (MQ=255) cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGAtt < 1:1942837/66‑1 (MQ=255) cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgt > 1:3967111/1‑70 (MQ=255) cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgtg > 1:2291290/1‑71 (MQ=255) cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgtg > 1:3208754/1‑71 (MQ=255) ttGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGAtt < 1:4861719/65‑1 (MQ=255) ttGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgtgt > 1:960298/1‑71 (MQ=255) gtgCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGtt < 1:3761349/70‑1 (MQ=255) gtgCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCTTAAAGATTCAGTGtt < 1:2385041/70‑1 (MQ=255) tgCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTaa < 1:2460855/71‑1 (MQ=255) gCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTaa < 1:3609935/70‑1 (MQ=255) ttACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCa < 1:2781101/59‑1 (MQ=255) ttACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAg < 1:1223627/60‑1 (MQ=255) aCGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCAt < 1:2816152/70‑1 (MQ=255) aCGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCAt < 1:4205231/70‑1 (MQ=255) aCGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:3733936/1‑71 (MQ=255) aCGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:494071/1‑71 (MQ=255) aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCa > 1:2147786/1‑57 (MQ=255) aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCAt < 1:3039878/70‑1 (MQ=255) aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:2553921/1‑71 (MQ=255) gTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcct < 1:2192168/71‑1 (MQ=255) gTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcct < 1:3772529/71‑1 (MQ=255) tCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcctt < 1:1383443/71‑1 (MQ=255) aCCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttac > 1:4235794/1‑71 (MQ=255) cgcgTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacctt < 1:587599/71‑1 (MQ=255) cgcgTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacct > 1:4918060/1‑70 (MQ=255) cgcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacct > 1:1367277/1‑70 (MQ=255) gcgTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacctta > 1:915988/1‑71 (MQ=255) cgTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTtat < 1:4808123/71‑1 (MQ=255) cgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTtat < 1:948791/71‑1 (MQ=255) cTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGc > 1:5139409/1‑70 (MQ=255) cTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGc > 1:4971907/1‑70 (MQ=255) tGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcctt < 1:4869881/59‑1 (MQ=255) tGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTtat < 1:1900689/66‑1 (MQ=255) tGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTtat < 1:3377885/66‑1 (MQ=255) tGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGc < 1:4280170/69‑1 (MQ=255) gCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGAc > 1:3587253/1‑71 (MQ=255) cGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGAcc > 1:496943/1‑51 (MQ=255) cGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGAc < 1:5082814/70‑1 (MQ=255) cGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTAAGTGTTAAGAcc > 1:1365890/1‑51 (MQ=255) cGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGAcc > 1:1244938/1‑71 (MQ=255) aaCTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttac > 1:4381254/1‑57 (MQ=255) aaCTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttac > 1:1356376/1‑57 (MQ=255) aaCTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCg < 1:3074737/71‑1 (MQ=255) aaCTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGc < 1:3404479/65‑1 (MQ=255) aaCTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGc < 1:1959081/65‑1 (MQ=255) cTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGga > 1:2750290/1‑71 (MQ=255) tACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGtt < 1:1013398/40‑1 (MQ=255) aCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:3639302/1‑70 (MQ=255) | CCATAAAGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGGAT > W3110S.gb/4000320‑4000458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |