| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 320,985 | A→G | 31.2% | I52V (ATC→GTC) | ykgE → | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 320,985 | 0 | A | G | 31.2% | 37.2 / 25.6 | 32 | I52V (ATC→GTC) | ykgE | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (12/10); new base G (5/5); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATT > W3110S.gb/320919‑321053 | ggCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATc < 1:3128124/69‑1 (MQ=255)ggCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCa > 1:3435041/1‑70 (MQ=255) cgTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGa < 1:4002617/71‑1 (MQ=255) aaTTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTc > 1:3679713/1‑70 (MQ=255) aaTTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTc > 1:336154/1‑70 (MQ=255) ccGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCC‑GGGATg < 1:1551044/71‑1 (MQ=255) ggAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCaa < 1:965841/51‑1 (MQ=255) gagaAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATGTCAAAGAAGCGATTCCAGGGATGa > 1:723157/1‑70 (MQ=255) gaAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATGTCAAAGAAGCGATTCCAGGGATGaaaa < 1:4881305/71‑1 (MQ=255) gaAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATGTCAAAGAAGCGATTCCAGGGATGaaaa < 1:4238419/71‑1 (MQ=255) gaAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGaaaa < 1:2750917/71‑1 (MQ=255) aCAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATc < 1:1004657/71‑1 (MQ=255) gATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGa > 1:5022302/1‑69 (MQ=255) gATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGa > 1:3914754/1‑69 (MQ=255) aTGCTGCGGTCAGCCTGCGATCAATAGCGGTTATGTCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcg < 1:3655279/71‑1 (MQ=255) gctgcGGTCAGCCTGCGATCAATAGCGGTTATGTCAAAGAAGCGa > 1:3763139/1‑45 (MQ=255) gctgcGGTCAGCCTGCGATCAATAGCGGTTATGTCAAAGAAGCGa > 1:1864117/1‑45 (MQ=255) cGGTCAGCCTGCGATCAATAGCGGTTATGTCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcgccgc < 1:904546/69‑1 (MQ=255) cGGTCAGCCTGCGATCAATAGCGGTTATGTCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcgccgc < 1:4646654/69‑1 (MQ=255) cGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcgccgc < 1:2026562/69‑1 (MQ=255) cGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCAc > 1:4221711/1‑71 (MQ=255) tCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATg < 1:3551376/49‑1 (MQ=255) tCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATg < 1:1690287/49‑1 (MQ=255) tCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcgc > 1:901895/1‑63 (MQ=255) cAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTg > 1:707840/1‑69 (MQ=255) cTGCGATCAATAGCGGTTATGTCAAAGAAGCGATTCCAGGGATGAAAAATCTg > 1:4618751/1‑53 (MQ=255) tGCGATCAATAGCGGTTATGTCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGAt > 1:2191141/1‑70 (MQ=255) tGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAggg < 1:945615/40‑1 (MQ=255) cGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAac > 1:4905873/1‑71 (MQ=255) cAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTggagg > 1:1340248/1‑62 (MQ=255) gCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATcc > 1:1500884/1‑70 (MQ=255) gTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGa > 1:853602/1‑39 (MQ=255) tatgTCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCatta < 1:3004184/70‑1 (MQ=255) tataTCAAAGAAGCGATTCCAGGGATGAAAAATCTGAt > 1:473340/1‑38 (MQ=255) tataTCAAAGAAGCGATTCCAGGGATGAAAAATCTGAt > 1:3741420/1‑38 (MQ=255) atatCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCattatt > 1:3234925/1‑71 (MQ=255) atatCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCattatt > 1:1964212/1‑71 (MQ=255) | GGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATT > W3110S.gb/320919‑321053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |