| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,010,113 | C→T | 10.7% | A100V (GCC→GTC) | yhiI → | predicted HlyD family secretion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,010,113 | 0 | C | T | 10.7% | 166.2 / 7.3 | 56 | A100V (GCC→GTC) | yhiI | predicted HlyD family secretion protein |
| Reads supporting (aligned to +/- strand): ref base C (26/24); new base T (3/3); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGATGGATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCGCTGG > W3110S.gb/4010043‑4010178 | aaGATGGATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAgcgc < 1:3014224/71‑1 (MQ=255)aaGATGGATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAgcgc < 1:2399943/71‑1 (MQ=255) gATGGATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCTCCg > 1:3530093/1‑71 (MQ=255) gATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAgcgc < 1:3625366/65‑1 (MQ=255) gATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTtgc < 1:2993423/71‑1 (MQ=255) gATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTtgc < 1:3149676/71‑1 (MQ=255) gATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTtgc < 1:1542088/71‑1 (MQ=255) tCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGcc < 1:4652704/61‑1 (MQ=255) tCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGcc < 1:3735454/61‑1 (MQ=255) cgTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCgcgc < 1:2539177/71‑1 (MQ=255) gtgtTGCCGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCgcgc < 1:3795146/70‑1 (MQ=255) ttGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGc > 1:1683902/1‑71 (MQ=255) gCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCtt > 1:2059731/1‑71 (MQ=255) gCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCtt > 1:2254388/1‑71 (MQ=255) gCAGGAACAGCGACTGGAAGCCATAGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCa > 1:2228373/1‑66 (MQ=255) cAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCttt < 1:2588139/71‑1 (MQ=255) aGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTg < 1:2447788/71‑1 (MQ=255) aGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTg < 1:4558587/71‑1 (MQ=255) ggAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGc < 1:4252275/71‑1 (MQ=255) ggAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGc < 1:3107915/71‑1 (MQ=255) gAACAGCGACTGGAATCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGc < 1:4737075/70‑1 (MQ=255) gAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCt > 1:1815928/1‑71 (MQ=255) aCAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCa > 1:1505972/1‑60 (MQ=255) aCAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTg > 1:1239272/1‑70 (MQ=255) aGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAg > 1:1825829/1‑71 (MQ=255) gCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAg > 1:2864963/1‑70 (MQ=255) cGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCttt < 1:2165142/62‑1 (MQ=255) cTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAAc > 1:1101423/1‑70 (MQ=255) tGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCtt < 1:299598/57‑1 (MQ=255) gAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGAc < 1:3981901/70‑1 (MQ=255) gAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGAc < 1:1110316/70‑1 (MQ=255) aGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACaaa < 1:2066160/71‑1 (MQ=255) cATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCg < 1:609781/71‑1 (MQ=255) cATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCg < 1:3558816/71‑1 (MQ=255) aTCGCGCAAATCAAAGAGGCACAAAGCGTCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGa < 1:4414450/71‑1 (MQ=255) aTCGCGCAAATCAAAGAGGCACAAAGCGTCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGa < 1:2892693/71‑1 (MQ=255) aTCGCGCAAATCAAAGAGGCACAAAGCGTCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGa < 1:1925375/71‑1 (MQ=255) tCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGa > 1:4577645/1‑70 (MQ=255) cgcgCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGaaa > 1:2856678/1‑71 (MQ=255) cgcgCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGaaa > 1:4071392/1‑71 (MQ=255) cgcgCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGaaa > 1:3660481/1‑71 (MQ=255) cgcgCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGaaa > 1:3740770/1‑71 (MQ=255) cgcgCAAATCAAAGAGGCA‑AAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAAc > 1:1600950/1‑71 (MQ=255) gcgcAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAAc > 1:727206/1‑71 (MQ=255) gcgcAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAAc < 1:4171337/71‑1 (MQ=255) aaaTCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGt < 1:3854653/71‑1 (MQ=255) aaaTCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGt < 1:1358695/71‑1 (MQ=255) aaaTCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGt < 1:424822/71‑1 (MQ=255) aTCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGaa > 1:3731850/1‑63 (MQ=255) aTCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGa < 1:425116/62‑1 (MQ=255) tCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTg > 1:1663671/1‑44 (MQ=255) aaaGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCg > 1:2785114/1‑70 (MQ=255) aaGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGca > 1:3559020/1‑71 (MQ=255) agagGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGca > 1:2657439/1‑70 (MQ=255) acaAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCg > 1:2629055/1‑70 (MQ=255) caAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCGCt < 1:3123983/71‑1 (MQ=255) aaGCGTCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCGCTgg > 1:4915363/1‑71 (MQ=255) aaGCGTCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCGCTg > 1:1483382/1‑70 (MQ=255) aaGCGTCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCGCTg > 1:1119359/1‑70 (MQ=255) aaGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCGCTgg > 1:441423/1‑71 (MQ=255) aaGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCGCTgg > 1:1291112/1‑71 (MQ=255) | AAGATGGATACTCGCGTGTTGCAGGAACAGCGACTGGAAGCCATCGCGCAAATCAAAGAGGCACAAAGCGCCGTTGCTGCCGCGCAGGCTTTGCTGGAGCAACGACAAAGCGAAACTCGTGCCGCACAGTCGCTGG > W3110S.gb/4010043‑4010178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |