| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,012,376 | G→A | 38.3% | A500T (GCC→ACC) | rbbA → | fused ribosome‑associated ATPases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,012,376 | 0 | G | A | 38.3% | 39.4 / 49.6 | 47 | A500T (GCC→ACC) | rbbA | fused ribosome‑associated ATPases |
| Reads supporting (aligned to +/- strand): ref base G (11/18); new base A (5/13); total (16/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAACGAAGCCGAAGC > W3110S.gb/4012306‑4012446 | cATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAg < 1:5024293/71‑1 (MQ=255) tcACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAgccgc > 1:255265/1‑71 (MQ=255) tcACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAaccgc > 1:334467/1‑71 (MQ=255) tGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAg < 1:2032104/70‑1 (MQ=255) aTGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGt < 1:3066322/69‑1 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTg > 1:1274045/1‑71 (MQ=255) cACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAaccgc > 1:3497927/1‑62 (MQ=255) aCGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa < 1:1135382/71‑1 (MQ=255) cGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTgg > 1:3114172/1‑68 (MQ=255) ccGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAagag > 1:2094731/1‑71 (MQ=255) ccGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAagag > 1:2100638/1‑71 (MQ=255) cGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTgg < 1:1037934/65‑1 (MQ=255) gAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCa > 1:2614174/1‑71 (MQ=255) aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:4728771/71‑1 (MQ=255) aGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTa < 1:4297851/71‑1 (MQ=255) aGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTa < 1:3160320/71‑1 (MQ=255) aGTGCTTGCCAGCGGTACACAGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCa < 1:4967222/67‑1 (MQ=255) aGTGCTTCCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTa < 1:4608984/71‑1 (MQ=255) cTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAtt < 1:1054447/65‑1 (MQ=255) cTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGc > 1:3880827/1‑71 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCt < 1:1681853/70‑1 (MQ=255) ccAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTa < 1:3867258/70‑1 (MQ=255) aGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTAttt < 1:1603358/71‑1 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga < 1:2617626/52‑1 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga < 1:676506/52‑1 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga < 1:1406316/52‑1 (MQ=255) cGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGc < 1:1546306/71‑1 (MQ=255) cGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGc < 1:3857376/71‑1 (MQ=255) cGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGc < 1:4763457/71‑1 (MQ=255) ggTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:4068386/57‑1 (MQ=255) ggTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCa < 1:1707215/71‑1 (MQ=255) gTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAg < 1:3919409/71‑1 (MQ=255) tACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCAtt < 1:2561131/54‑1 (MQ=255) cacCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAgg > 1:3050060/1‑69 (MQ=255) cGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGG‑AGAGGCATTTATCGCCTATTTGCAGGAAGCgg > 1:3995486/1‑71 (MQ=255) gCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTAtt > 1:3105268/1‑59 (MQ=255) aGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCt < 1:3276122/54‑1 (MQ=255) aaCTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:1289206/1‑71 (MQ=255) aCTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAaga > 1:3608028/1‑36 (MQ=255) cTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg > 1:3310428/1‑69 (MQ=255) tGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTAttt < 1:2139214/52‑1 (MQ=255) gTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCa < 1:640100/36‑1 (MQ=255) agaAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAc < 1:4635623/71‑1 (MQ=255) gaAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAc < 1:855202/70‑1 (MQ=255) gaAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcg < 1:124894/71‑1 (MQ=255) aaaCGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcg > 1:3127693/1‑70 (MQ=255) cgcgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggca > 1:181098/1‑54 (MQ=255) cgcgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggca > 1:1522819/1‑54 (MQ=255) cgcgGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggca > 1:638170/1‑54 (MQ=255) cgcgGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAc < 1:3024142/66‑1 (MQ=255) gtaGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGc < 1:3095655/45‑1 (MQ=255) gccgccAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAAcgaagccgaagc > 1:1852442/1‑71 (MQ=255) | CATCTCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAACGAAGCCGAAGC > W3110S.gb/4012306‑4012446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |