| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,014,473 | T→C | 35.6% | L287P (CTG→CCG) | yhhJ → | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,014,473 | 0 | T | C | 35.6% | 53.5 / 61.1 | 59 | L287P (CTG→CCG) | yhhJ | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/24); new base C (11/10); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GCGCTCAGTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCACGCCGCGCGAAAG > W3110S.gb/4014403‑4014542 | gcgcTCAGTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACt > 1:4313393/1‑71 (MQ=255) gcTCAGTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCgg < 1:3911239/71‑1 (MQ=255) gTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTggg < 1:1434980/67‑1 (MQ=255) gTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTggg < 1:644904/67‑1 (MQ=255) gTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTggg < 1:3046473/67‑1 (MQ=255) gTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTggg < 1:2615233/67‑1 (MQ=255) gTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGgctg < 1:2730644/71‑1 (MQ=255) tCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCgg < 1:4688639/65‑1 (MQ=255) tttGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGACGCAACCGGGGCTGCTGGt > 1:738089/1‑71 (MQ=255) tttGCCACCAAGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGt > 1:4913426/1‑71 (MQ=255) gCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGa < 1:4101701/70‑1 (MQ=255) caccacGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGATTc < 1:660510/71‑1 (MQ=255) accacGTCAATCGTCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCt < 1:4182504/71‑1 (MQ=255) accacGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCt > 1:2402943/1‑71 (MQ=255) ccacGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGgc < 1:2948500/57‑1 (MQ=255) cacGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTgg < 1:1570728/71‑1 (MQ=255) acGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTTATTCTGGt < 1:1065021/71‑1 (MQ=255) acGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGt < 1:4420847/71‑1 (MQ=255) acGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGATTCTGGt > 1:4074408/1‑71 (MQ=255) acGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGATTCTGGt > 1:1760929/1‑71 (MQ=255) tCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGtgct < 1:1246586/71‑1 (MQ=255) cAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGt > 1:883883/1‑67 (MQ=255) aaTCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGtgctgc > 1:4506534/1‑71 (MQ=255) aTCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGtgctgct < 1:744228/71‑1 (MQ=255) cGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGtgctgctgc > 1:674519/1‑71 (MQ=255) cGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGtgctgctgc > 1:2359385/1‑71 (MQ=255) cGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGtgctgctg < 1:4970648/70‑1 (MQ=255) ggCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTgg > 1:4386631/1‑61 (MQ=255) gCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGgctgct < 1:3995199/49‑1 (MQ=255) aTTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTgg > 1:4594172/1‑49 (MQ=255) tttttATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGAt < 1:4844416/52‑1 (MQ=255) tttATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTgg > 1:610627/1‑55 (MQ=255) tttATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTgg > 1:388465/1‑55 (MQ=255) tttATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGc > 1:1127730/1‑70 (MQ=255) tttATGGGGACGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGc > 1:3479326/1‑70 (MQ=255) tttATGGGGAAGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGc > 1:4804099/1‑70 (MQ=255) ttATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCa < 1:2696636/70‑1 (MQ=255) cGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGaaaa > 1:2287870/1‑63 (MQ=255) cGATAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTcc < 1:5044003/71‑1 (MQ=255) aTAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCa < 1:241855/59‑1 (MQ=255) aTAGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCgg > 1:2683076/1‑71 (MQ=255) tAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCggt < 1:4142518/71‑1 (MQ=255) aGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCggt < 1:2364747/70‑1 (MQ=255) aGCGCGTTCAATGCCGCAACCGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCg > 1:3431879/1‑68 (MQ=255) cgcgTTCAATGCCGCAACCGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCggtgg < 1:44857/70‑1 (MQ=255) gcgTTAAATGCCGCAACTGGGGCTGCTGGTGATTCTg > 1:2112826/1‑37 (MQ=255) cgTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTc < 1:4520510/71‑1 (MQ=255) gTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTcc < 1:1461949/62‑1 (MQ=255) ttCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCa < 1:4719883/71‑1 (MQ=255) tCAATGCCGCAACTGGGGCTGCTTGTGATTCTGGTGCTGCTGCCGCTGCAAATGCtt < 1:909855/57‑1 (MQ=255) aTGCCGCAACTGGGGCTTCTGGTGATTCTGGtgctgc > 1:1516316/1‑37 (MQ=255) aTGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCAcgcc > 1:2196210/1‑71 (MQ=255) tGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCa < 1:1269387/46‑1 (MQ=255) tGCCGCAACCGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCa > 1:2089289/1‑66 (MQ=255) ccGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCaaa > 1:267996/1‑46 (MQ=255) ccGCAACCGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCACGCcgcg > 1:3982046/1‑71 (MQ=255) cGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAAt < 1:3072507/46‑1 (MQ=255) cTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCACGCCGCGCGAAAg < 1:4913014/71‑1 (MQ=255) cTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCACGCCGCGCGAAAg < 1:2978083/71‑1 (MQ=255) cTGCGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCACGCCGCGCGAAAg < 1:62191/71‑1 (MQ=255) tGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCACGCCGCGCGAAAg > 1:4180407/1‑70 (MQ=255) | GCGCTCAGTCTGTTTGCCACCACGTCAATCGGCATTTTTATGGGGACGATAGCGCGTTCAATGCCGCAACTGGGGCTGCTGGTGATTCTGGTGCTGCTGCCGCTGCAAATGCTTTCCGGTGGTTCCACGCCGCGCGAAAG > W3110S.gb/4014403‑4014542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |