| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,017,703 | T→C | 45.5% | N1175D (AAC→GAC) | rhsB ← | rhsB element core protein RshB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,017,703 | 0 | T | C | 45.5% | 4.6 / 43.5 | 33 | N1175D (AAC→GAC) | rhsB | rhsB element core protein RshB |
| Reads supporting (aligned to +/- strand): ref base T (10/8); new base C (3/12); total (13/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
ATCATACTGCTGCCCCGGCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAG > W3110S.gb/4017635‑4017757 | atcatACTGCTGCCCCGGCAGCCGGATAAGCTGCTGCAACTGATGCGGGTTCTCTTCATTCAGCAGGTCGc > 1:1099927/1‑71 (MQ=255) atACTGCTGCCCCGGCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGccc > 1:3907729/1‑70 (MQ=35) ctgcCCCGGCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATtc < 1:831073/69‑1 (MQ=35) ccGGCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCata > 1:928128/1‑70 (MQ=40) ccGGCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCata > 1:1380843/1‑70 (MQ=40) gCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCt > 1:377114/1‑71 (MQ=39) aGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTgc > 1:3588097/1‑71 (MQ=255) cGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATtcatca < 1:189636/60‑1 (MQ=31) gATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGcac < 1:3652700/70‑1 (MQ=35) gATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTTCGcacc < 1:2101466/71‑1 (MQ=255) gctgctGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCt < 1:3004132/71‑1 (MQ=40) ctgctgCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTg < 1:5140168/71‑1 (MQ=255) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCata < 1:2391709/51‑1 (MQ=35) cAGCTGATGCGGCTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGc < 1:2542781/69‑1 (MQ=255) aGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGcccc < 1:5062214/71‑1 (MQ=255) gATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTcc > 1:3159162/1‑71 (MQ=39) aTGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCg < 1:5132986/71‑1 (MQ=39) aTGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGcc < 1:609819/64‑1 (MQ=255) tGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTc < 1:4061411/44‑1 (MQ=255) gggTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCt < 1:1076901/71‑1 (MQ=255) gggTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCt < 1:2632357/71‑1 (MQ=255) ttCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTc < 1:3621687/38‑1 (MQ=40) tctcTTCATTCAGCAGGTCG‑CCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCtgat > 1:4463963/1‑69 (MQ=14) ctctTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCtgatg < 1:295420/70‑1 (MQ=255) ctctTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCtgatg < 1:4956163/70‑1 (MQ=255) ttcattcaGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTg < 1:2364806/61‑1 (MQ=255) ttcattcaGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCt < 1:1873844/63‑1 (MQ=255) cattcaGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTg > 1:4488858/1‑59 (MQ=35) cattcaGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTg > 1:3633454/1‑59 (MQ=35) cattcaGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTg > 1:1288828/1‑59 (MQ=35) cattcaGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCt > 1:4755283/1‑61 (MQ=35) cattcaGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCt > 1:1620390/1‑61 (MQ=35) ttcaGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAg < 1:2266019/65‑1 (MQ=14) | ATCATACTGCTGCCCCGGCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAG > W3110S.gb/4017635‑4017757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |