| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,027,859 | A→G | 51.7% | L232L (TTG→CTG) | yhhT ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,027,859 | 0 | A | G | 51.7% | 6.5 / 85.6 | 60 | L232L (TTG→CTG) | yhhT | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (9/20); new base G (15/16); total (24/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01 | |||||||||||
AACAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGAT > W3110S.gb/4027789‑4027925 | aaCAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCaa < 1:69060/71‑1 (MQ=255) aCAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCaaa < 1:3469835/71‑1 (MQ=255) agcagcACCTGAATCATTGGCGGTACGGCGGAAATAACCGCGCCGATATTGGGCACGTAGTTGAGCaaa < 1:3881294/69‑1 (MQ=255) aCCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAg < 1:3268840/71‑1 (MQ=255) tGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTAc > 1:1222334/1‑71 (MQ=255) gAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTAc > 1:4854627/1‑70 (MQ=255) aaTCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTAc > 1:2857977/1‑69 (MQ=255) aaTCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTAc > 1:3918706/1‑69 (MQ=255) aaTCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTAc > 1:4013605/1‑69 (MQ=255) aaTCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTg > 1:160493/1‑71 (MQ=255) aTCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGc > 1:5072886/1‑71 (MQ=255) aTCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGc > 1:1295605/1‑71 (MQ=255) tCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGcc < 1:334807/71‑1 (MQ=255) aTTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCagaaa > 1:2753381/1‑56 (MQ=255) aTTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGGTGAGCAGAAACGCCAGTACTGccc < 1:1339628/70‑1 (MQ=255) ttGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTAATGCCcac < 1:3914080/71‑1 (MQ=255) ggTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTAc > 1:1344115/1‑59 (MQ=255) tACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGc < 1:2393882/51‑1 (MQ=255) tACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAgcg > 1:719037/1‑71 (MQ=255) gggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAg < 1:4559918/51‑1 (MQ=255) cggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAg < 1:418119/52‑1 (MQ=255) cggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAgcgc < 1:4126840/70‑1 (MQ=255) cggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCa < 1:2698973/71‑1 (MQ=255) cggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCa < 1:1489241/71‑1 (MQ=255) ggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCaa < 1:4629231/71‑1 (MQ=255) ggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCa < 1:2319915/70‑1 (MQ=255) ggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAg < 1:3896078/48‑1 (MQ=255) gAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTg > 1:966569/1‑71 (MQ=255) gAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTg < 1:5103954/71‑1 (MQ=255) aaaTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGcc < 1:3361495/44‑1 (MQ=255) aaaTTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGc < 1:3771015/43‑1 (MQ=255) atttACCGCGCGGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTAc < 1:1843043/46‑1 (MQ=255) aaTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGcc < 1:1911249/43‑1 (MQ=255) aTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAg > 1:2748294/1‑44 (MQ=255) aTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAg > 1:887341/1‑44 (MQ=255) aTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAg > 1:757069/1‑44 (MQ=255) aTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACt > 1:4663505/1‑48 (MQ=255) aTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACAt > 1:1868144/1‑56 (MQ=255) aTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAc < 1:882568/71‑1 (MQ=255) aCCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAcccc > 1:758770/1‑71 (MQ=255) ccGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAccccc < 1:1462644/71‑1 (MQ=255) cgcgCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAccccc < 1:2821623/70‑1 (MQ=255) cgcgCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAg < 1:3260992/39‑1 (MQ=255) cgcCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGccc < 1:1758506/45‑1 (MQ=255) cgcCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTa > 1:2684442/1‑39 (MQ=255) cGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGc < 1:2090718/71‑1 (MQ=255) gATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCt < 1:1463047/71‑1 (MQ=255) atatTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCa > 1:1887930/1‑58 (MQ=255) atTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCa < 1:4353924/49‑1 (MQ=255) ttGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTg < 1:1150644/53‑1 (MQ=255) ttGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCTGCTcca < 1:8796/70‑1 (MQ=255) tGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTccagc > 1:1225265/1‑71 (MQ=255) cGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTccagccccagc < 1:579109/71‑1 (MQ=255) gTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTg < 1:4183288/45‑1 (MQ=255) aGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCa > 1:1331213/1‑45 (MQ=255) gTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGa > 1:472091/1‑71 (MQ=255) gTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCccagccag < 1:174160/70‑1 (MQ=255) ttGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAcccc < 1:1204883/47‑1 (MQ=255) tGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGAc < 1:4276864/70‑1 (MQ=255) tGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGAc > 1:4135428/1‑70 (MQ=255) cgCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGc < 1:2093218/38‑1 (MQ=255) aGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGAt < 1:2954465/71‑1 (MQ=255) cAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGAt < 1:4939183/69‑1 (MQ=255) cAAAAACGCCAGTACTGCCCACATCAGCGCAAACAGCAcccc > 1:2436164/1‑42 (MQ=255) | AACAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGAT > W3110S.gb/4027789‑4027925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |