| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,036,723 | G→A | 37.5% | A184T (GCT→ACT) | ftsY → | fused Signal Recognition Particle (SRP) receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,036,723 | 0 | G | A | 37.5% | 27.1 / 31.0 | 32 | A184T (GCT→ACT) | ftsY | fused Signal Recognition Particle (SRP) receptor |
| Reads supporting (aligned to +/- strand): ref base G (9/11); new base A (5/7); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
CCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACC > W3110S.gb/4036655‑4036791 | ccGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGc < 1:1683887/70‑1 (MQ=255) gAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACt > 1:4021748/1‑69 (MQ=255) gATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGAcc > 1:950380/1‑70 (MQ=255) caTGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGAcc > 1:3848188/2‑70 (MQ=255) aTGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaa < 1:3135866/71‑1 (MQ=255) tggtggTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaaa < 1:1893644/71‑1 (MQ=255) gtggtTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAaaga < 1:3008818/71‑1 (MQ=255) tgggTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAaaga < 1:91909/70‑1 (MQ=255) gtTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAaagaa > 1:437451/1‑69 (MQ=255) cctccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACt < 1:4401237/39‑1 (MQ=255) cctccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACt < 1:3956662/39‑1 (MQ=255) ccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAaag > 1:2354974/1‑61 (MQ=255) cAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGaaa < 1:2177632/45‑1 (MQ=255) agaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGcc > 1:553094/1‑71 (MQ=255) agagCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGa < 1:487948/71‑1 (MQ=255) cagcCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt < 1:2593332/55‑1 (MQ=255) gcCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCa < 1:1665310/71‑1 (MQ=255) ggTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGcc > 1:1095138/1‑71 (MQ=255) ggTGCAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGcc > 1:1704696/1‑71 (MQ=255) gaagaaATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGt > 1:2680586/1‑70 (MQ=255) aagaaATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTa < 1:2594359/71‑1 (MQ=255) aagaaATCACTCAGGAGCAGGAAAAACCGACCAaagaa < 1:2907506/38‑1 (MQ=255) agaaATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTa > 1:3621432/1‑70 (MQ=255) gaaATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTaaa < 1:2804656/71‑1 (MQ=255) gaaATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTaaa < 1:1480804/71‑1 (MQ=255) aaaTCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAAGGCAGCCTGTTaaa > 1:2800461/1‑70 (MQ=255) aaaTCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCa < 1:5057443/60‑1 (MQ=255) aaTCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGttt < 1:4021703/39‑1 (MQ=255) aTCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAAc > 1:215295/1‑71 (MQ=255) tCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACTCAGCCTGTTAAAAAcc < 1:4192899/71‑1 (MQ=255) cGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGtttttt > 1:1732623/1‑39 (MQ=255) cGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGtttttt > 1:4068397/1‑39 (MQ=255) | CCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACC > W3110S.gb/4036655‑4036791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |