| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 330,092 | A→G | 10.1% | N469S (AAC→AGC) | betT → | choline transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 330,092 | 0 | A | G | 10.1% | 180.7 / 12.4 | 69 | N469S (AAC→AGC) | betT | choline transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base A (38/24); new base G (3/4); total (41/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
CGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGT > W3110S.gb/330027‑330161 | cGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGa > 1:2924922/1‑71 (MQ=255)cGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGa > 1:1835826/1‑71 (MQ=255) gctgtctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAGCGGGata < 1:2339088/71‑1 (MQ=255) ctggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAGCGGGata < 1:1460070/70‑1 (MQ=255) tcttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGat < 1:4965660/58‑1 (MQ=255) tcttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGat < 1:3653136/58‑1 (MQ=255) tcttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGat < 1:5116791/58‑1 (MQ=255) tcttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaa < 1:5106750/71‑1 (MQ=255) cttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaa > 1:898861/1‑70 (MQ=255) cttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaa > 1:4924161/1‑70 (MQ=255) cttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaa > 1:1526735/1‑70 (MQ=255) ttctGGTTGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaaaa > 1:3133951/1‑71 (MQ=255) ttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAGCGGGATATCCGCGCTGCaaaa > 1:1938616/1‑71 (MQ=255) ttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGatat > 1:848404/1‑58 (MQ=255) tctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaaaa > 1:3024220/1‑70 (MQ=255) ctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaaaa < 1:276323/69‑1 (MQ=255) ctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaaaa < 1:2609937/69‑1 (MQ=255) ctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaaaa > 1:915596/1‑69 (MQ=255) tGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCgcgc > 1:4318502/1‑61 (MQ=255) cGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACg > 1:4984309/1‑71 (MQ=255) ggTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATc < 1:1592853/51‑1 (MQ=255) tGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCaa < 1:3887684/59‑1 (MQ=255) tGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGt > 1:2501163/1‑70 (MQ=255) gCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGAt < 1:1002311/71‑1 (MQ=255) cGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAGCGGGATATCCGCGCTGCAAAACACCACGGTGa < 1:3711131/69‑1 (MQ=255) ttGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATg > 1:4420319/1‑71 (MQ=255) tGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATgg > 1:1791775/1‑71 (MQ=255) tGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATgg > 1:1672833/1‑71 (MQ=255) tGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATgg > 1:3065143/1‑71 (MQ=255) tGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATgg > 1:1298398/1‑71 (MQ=255) ggCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAac > 1:1129886/1‑54 (MQ=255) gCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATggg < 1:2927228/70‑1 (MQ=255) gCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATggg < 1:4068738/70‑1 (MQ=255) gCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATggg < 1:3808144/70‑1 (MQ=255) ctgctgACGCTCTGCATGCTGATGACTAGCGGGATATCCGCGCTGCAAAACACCACGGTGa < 1:948929/61‑1 (MQ=255) tgctgcCGCTCGGCATGCTGATGACTCGCGGGATATCCGCGCTGCAAAACACCACg > 1:2712053/1‑56 (MQ=38) tgctgACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAaca < 1:3225911/51‑1 (MQ=255) tgctgACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGa > 1:652275/1‑60 (MQ=255) gctgACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGc > 1:2143127/1‑71 (MQ=255) gctgACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGc > 1:2978430/1‑71 (MQ=255) tgACGCTCGGCATGCTGATGACTAACGGGATATcc < 1:2315220/35‑1 (MQ=255) aCGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGc < 1:2225468/40‑1 (MQ=255) tCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGc > 1:1346112/1‑60 (MQ=255) gCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGa < 1:3528516/48‑1 (MQ=255) gCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTc < 1:498865/65‑1 (MQ=255) gCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTc < 1:4856152/65‑1 (MQ=255) gCATGCTGATGACTAACGGGATATCCGAGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTc < 1:1572894/65‑1 (MQ=255) cATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGa < 1:5136570/47‑1 (MQ=255) aTGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGa > 1:640192/1‑46 (MQ=255) aTGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGa > 1:4314281/1‑46 (MQ=255) aTGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGAtt > 1:629944/1‑48 (MQ=255) aTGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTtg > 1:3370340/1‑70 (MQ=255) tGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTtgtg < 1:3609151/71‑1 (MQ=255) gCTGATGACTAACGGGATATCGGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTc > 1:994876/1‑61 (MQ=255) gCTGATGACTAACGGGATATCGGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTc > 1:988807/1‑61 (MQ=255) gCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTc > 1:4696671/1‑61 (MQ=255) gCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTc > 1:3030795/1‑61 (MQ=255) cTGATGACTAGCGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGAt > 1:2914038/1‑71 (MQ=255) cTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGAtt > 1:1235657/1‑45 (MQ=255) cTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGAtt > 1:1963178/1‑45 (MQ=255) cTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGAt > 1:4853154/1‑71 (MQ=255) gatgaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGAtct > 1:2339667/1‑71 (MQ=255) atgaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGAtc < 1:243587/69‑1 (MQ=255) tgaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCg < 1:5128455/53‑1 (MQ=255) gaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATg > 1:4630849/1‑43 (MQ=255) gaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGAtcttct > 1:2431254/1‑71 (MQ=255) aCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATg > 1:3905013/1‑42 (MQ=255) tAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGAtcttc < 1:1714062/67‑1 (MQ=255) tAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCg > 1:4595295/1‑71 (MQ=255) aaCGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGt > 1:4558987/1‑71 (MQ=255) aaCGGGATATCCGCGCTGCAAAACACAACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGt > 1:3433511/1‑71 (MQ=255) aCGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGt < 1:1358651/70‑1 (MQ=255) aCGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGt < 1:2681546/70‑1 (MQ=255) aCGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGt < 1:2934057/70‑1 (MQ=255) aCGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTCCTTCGt < 1:2341/70‑1 (MQ=255) | CGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGT > W3110S.gb/330027‑330161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |