| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,083,312 | G→A | 52.6% | G325E (GGG→GAG) | rtcB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,083,312 | 0 | G | A | 52.6% | 1.6 / 82.4 | 57 | G325E (GGG→GAG) | rtcB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (14/13); new base A (15/15); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCG > W3110S.gb/4083245‑4083372 | tCTGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCt < 1:3018480/71‑1 (MQ=255) tGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTgg > 1:4356560/1‑71 (MQ=255) tGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTgg > 1:1308884/1‑71 (MQ=255) tGCTGGTCAATATGGTATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATg < 1:2265951/71‑1 (MQ=255) tGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATg < 1:3506258/71‑1 (MQ=255) tGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATg < 1:2575060/71‑1 (MQ=255) tGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATg < 1:1306675/71‑1 (MQ=255) tGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGc < 1:1345903/62‑1 (MQ=255) tGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGc < 1:1239710/62‑1 (MQ=255) gTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGa < 1:2544524/67‑1 (MQ=255) cAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGt > 1:3929067/1‑70 (MQ=255) atatGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGa < 1:2039340/58‑1 (MQ=255) atGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGa < 1:2725881/61‑1 (MQ=255) tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGaaa > 1:4482132/1‑57 (MQ=255) tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGa < 1:3853478/55‑1 (MQ=255) tGGAATGATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCt > 1:3723/1‑71 (MQ=255) gAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTg < 1:1011882/70‑1 (MQ=255) gAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTg < 1:407705/70‑1 (MQ=255) gAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGt > 1:4975784/1‑71 (MQ=255) gAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGt < 1:1349487/71‑1 (MQ=255) gAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGt < 1:548698/71‑1 (MQ=255) gAATTATTCCCGGTTCGAAGGGAGCAAAAAGCTCTATCGTCCGTGAGCTGGGaaa > 1:4941830/1‑55 (MQ=255) aaTTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGt < 1:2712211/70‑1 (MQ=255) aaTTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGt < 1:2828318/70‑1 (MQ=255) attCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCg < 1:1443118/60‑1 (MQ=255) attCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCt > 1:3822710/1‑64 (MQ=255) attCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTg > 1:2896035/1‑71 (MQ=255) attCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGt > 1:2937150/1‑70 (MQ=255) ttCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg < 1:3611824/70‑1 (MQ=255) ggTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCa > 1:954968/1‑71 (MQ=255) gTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCAc < 1:2583745/71‑1 (MQ=255) gTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCAc < 1:4728855/71‑1 (MQ=255) gTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCAc > 1:906244/1‑71 (MQ=255) cGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAaga > 1:1332207/1‑46 (MQ=255) cGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACgg > 1:106536/1‑70 (MQ=255) cGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGt > 1:441656/1‑71 (MQ=255) cGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGt > 1:2968846/1‑71 (MQ=255) gggAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGt < 1:2756427/47‑1 (MQ=255) gggAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGt > 1:2838096/1‑52 (MQ=255) gggAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGt > 1:2671391/1‑52 (MQ=255) gggAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGt > 1:4045080/1‑52 (MQ=255) gggAGCAAAAAGCTTTATCCTCCGTGAGCTGGGAAATGAaga > 1:2334634/1‑42 (MQ=38) aaaaaGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCa > 1:1996422/1‑53 (MQ=255) aaaaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGt < 1:3146834/71‑1 (MQ=255) aaaaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGt < 1:1955617/71‑1 (MQ=255) aaaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg > 1:1685073/1‑49 (MQ=255) aaaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg > 1:1679105/1‑49 (MQ=255) aaaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg > 1:1097858/1‑49 (MQ=255) aaGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAg < 1:1909460/51‑1 (MQ=255) aaGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACg > 1:2073858/1‑56 (MQ=255) aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTaa > 1:1187928/1‑71 (MQ=255) aGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCAc < 1:3677627/54‑1 (MQ=255) aGCTTTATCGTCCGTGAGCTGGTAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGTTGCCGGGCGGGTAAt > 1:764150/1‑71 (MQ=255) tATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg < 1:2379325/42‑1 (MQ=255) tCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAg > 1:535293/1‑43 (MQ=255) tCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAg > 1:907776/1‑43 (MQ=255) tCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCg < 1:1019580/70‑1 (MQ=255) | TCTGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCG > W3110S.gb/4083245‑4083372 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |