| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,093,241 | C→A | 33.8% | G207C (GGT→TGT) | gntT ← | gluconate transporter, high‑affinity GNT I system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,093,241 | 0 | C | A | 33.8% | 113.9 / 43.9 | 62 | G207C (GGT→TGT) | gntT | gluconate transporter, high‑affinity GNT I system |
| Reads supporting (aligned to +/- strand): ref base C (27/14); new base A (12/9); total (39/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
AGGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCGG > W3110S.gb/4093175‑4093310 | agGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTcc > 1:4619836/1‑71 (MQ=255)agGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTcc > 1:4840075/1‑71 (MQ=255) gTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCgg < 1:3914487/71‑1 (MQ=255) gTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCgg < 1:1703834/71‑1 (MQ=255) cAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGaa < 1:3425335/70‑1 (MQ=255) cAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGaa < 1:2101210/70‑1 (MQ=255) cAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAAt > 1:3097561/1‑71 (MQ=255) cAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAAt > 1:4627173/1‑71 (MQ=255) aGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAAtt < 1:2751576/71‑1 (MQ=255) aGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAAtt < 1:4680670/71‑1 (MQ=255) gACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTg < 1:3934119/71‑1 (MQ=255) gACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTg > 1:2931331/1‑71 (MQ=255) cTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCtt < 1:2999338/71‑1 (MQ=255) cTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCt < 1:670034/70‑1 (MQ=255) gACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTc > 1:204591/1‑57 (MQ=255) gACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTc > 1:4986590/1‑57 (MQ=255) gACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTAt > 1:2604012/1‑71 (MQ=255) aCGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCTGGAATTGGCTTATc < 1:5121220/71‑1 (MQ=255) gCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTc > 1:905586/1‑54 (MQ=255) aaaGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAAt > 1:5134244/1‑57 (MQ=255) aaGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTcc > 1:5049005/1‑51 (MQ=255) aGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCt > 1:4179592/1‑71 (MQ=255) gCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCtt > 1:3338791/1‑71 (MQ=255) ggCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCaa < 1:22229/71‑1 (MQ=255) aTCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAacac > 1:4470631/1‑71 (MQ=255) tctcTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCt < 1:4321719/51‑1 (MQ=255) tctcTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACAcg > 1:2280444/1‑71 (MQ=255) tctcTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACAcg > 1:296778/1‑71 (MQ=255) tcttctTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTcc > 1:3380668/1‑39 (MQ=255) tcttctTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAt > 1:3447776/1‑44 (MQ=255) tcttctTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACAcgc > 1:1391053/1‑70 (MQ=255) tcttctACGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACAcgc > 1:4904/1‑70 (MQ=255) cttcttCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGa < 1:3801310/41‑1 (MQ=255) cttcttCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACAcgc > 1:950647/1‑69 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGa > 1:3502966/1‑50 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGa > 1:1993464/1‑50 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTg > 1:1752095/1‑41 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGAACTTCCGGAATTGGCTTATCGa > 1:3465587/1‑50 (MQ=255) cGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCg < 1:3851316/68‑1 (MQ=255) gCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTaa < 1:2004227/70‑1 (MQ=255) aaGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccgg < 1:3058780/71‑1 (MQ=255) ggTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGAt > 1:4955531/1‑42 (MQ=255) gTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggacc < 1:4207805/71‑1 (MQ=255) gTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGa < 1:1214498/40‑1 (MQ=255) gTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGa < 1:654214/40‑1 (MQ=255) ttCGCGCTTTAGAGACCTTCCGGAATTGGCTTATc > 1:2233942/1‑35 (MQ=255) ttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATc > 1:828605/1‑35 (MQ=255) ttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGc > 1:1451243/1‑71 (MQ=255) ttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGc > 1:4214268/1‑71 (MQ=255) gcgcTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCgag < 1:2707454/71‑1 (MQ=255) cgcTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCgaga < 1:2625757/71‑1 (MQ=255) tGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGATCGTa < 1:4758766/52‑1 (MQ=255) tAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCAc > 1:2332543/1‑71 (MQ=255) agagACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACg > 1:4478037/1‑71 (MQ=255) gagaCCTTCCGGAATTGGCTTATCGATACCTTTCaa > 1:3777984/1‑36 (MQ=255) gagaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACgg > 1:3598658/1‑71 (MQ=255) gagaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACgg > 1:4555149/1‑71 (MQ=255) gagaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACg < 1:2205894/70‑1 (MQ=255) gagaCATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAg > 1:526731/1‑44 (MQ=255) gaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTc > 1:4525278/1‑71 (MQ=255) gaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTc > 1:1594330/1‑71 (MQ=255) gaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTc > 1:1284793/1‑71 (MQ=255) ccTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCgg < 1:1888529/71‑1 (MQ=255) ccTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCgg < 1:1751004/71‑1 (MQ=255) ccTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCgg < 1:3870140/71‑1 (MQ=255) cTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCgaga < 1:5092353/59‑1 (MQ=255) cTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCgg > 1:3044735/1‑70 (MQ=255) | AGGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCGG > W3110S.gb/4093175‑4093310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |