| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,178,679 | A→G | 40.8% | L91S (TTA→TCA) | gspH ← | predicted general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,178,679 | 0 | A | G | 40.8% | 23.3 / 59.3 | 49 | L91S (TTA→TCA) | gspH | predicted general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base A (15/14); new base G (11/9); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
AAGATGAATTGAGAGTTCTTCATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATGCGCCATGCTGAATC > W3110S.gb/4178618‑4178740 | aaGATGAATTGAGAGTTCTTCATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAAcc < 1:772486/70‑1 (MQ=255) gAATTGAGAGTTCTTCATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAAcc < 1:866673/65‑1 (MQ=255) gAATTGAGAGTTCTTCATCCCAGTCATTTTGGCTCTAGTCTGCGGCATCTTCTTGTAATGGAACCCAgcgc > 1:3312424/1‑71 (MQ=255) aaTTGAGAGTTCTTCATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAgcgc < 1:1269215/70‑1 (MQ=255) gagaGTTCTTCATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGc > 1:2765101/1‑71 (MQ=255) agTTCTTCATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGa < 1:3676630/71‑1 (MQ=255) gTTCTTCATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGAt < 1:3142436/71‑1 (MQ=255) cttcATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGa > 1:4007256/1‑50 (MQ=255) cttcATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGa > 1:3937387/1‑50 (MQ=255) ttcATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGATgg < 1:3867459/69‑1 (MQ=255) cATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTaa > 1:2121849/1‑43 (MQ=255) cATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGATGGTGtt > 1:1865437/1‑71 (MQ=255) cATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGATGGTGtt > 1:2084438/1‑71 (MQ=255) cATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGATGGTGtt > 1:2178890/1‑71 (MQ=255) cATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGgtg < 1:4598584/69‑1 (MQ=255) tCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGtttt < 1:690608/71‑1 (MQ=255) gTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCg > 1:3786456/1‑71 (MQ=255) cATTTTGGCTCTCGTCTGCGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGa > 1:310131/1‑55 (MQ=255) tttGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGttt > 1:4771209/1‑60 (MQ=255) tttGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGttt > 1:1935881/1‑60 (MQ=255) ttGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAAcc > 1:1565501/1‑37 (MQ=255) ttGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCa > 1:4324519/1‑71 (MQ=255) ttGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCa > 1:2302734/1‑71 (MQ=255) ggCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCAt < 1:4918959/70‑1 (MQ=255) ggCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCAt < 1:605773/70‑1 (MQ=255) ggCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCAt < 1:3195425/70‑1 (MQ=255) ctctCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGgtgt < 1:4693780/53‑1 (MQ=255) ctcGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCAt < 1:3541452/66‑1 (MQ=255) ctcGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCAt < 1:3547610/66‑1 (MQ=255) ctcGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATgc > 1:302940/1‑71 (MQ=255) ctcGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATgc > 1:1359469/1‑71 (MQ=255) tGCGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATGCGCCAt > 1:2730522/1‑70 (MQ=255) cGGCATCTTCTTGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATGCGCCAt < 1:2366005/68‑1 (MQ=255) gCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATGCGCCATGCTGa < 1:3749507/71‑1 (MQ=255) tcttctTGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCAt < 1:685045/53‑1 (MQ=255) tcttctTGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCAt < 1:993941/53‑1 (MQ=255) tcttctTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGccc < 1:3953332/45‑1 (MQ=255) tcttctTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGccc < 1:1180640/45‑1 (MQ=255) cttcttGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTg < 1:3172471/41‑1 (MQ=255) cttcttGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTg < 1:1092834/41‑1 (MQ=255) ttcttGTGATGGAACCCAGCGCCAGGCTGATGGTGttt > 1:775793/1‑38 (MQ=255) ttcttGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATaa > 1:2244080/1‑53 (MQ=255) ttcttGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATGCGCCATGCTGAAt > 1:1262386/1‑68 (MQ=255) ttcttGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATaa > 1:4638304/1‑53 (MQ=255) ttcttGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATaa > 1:1851245/1‑53 (MQ=255) ttcttGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATGCGCCATGCTGAAt > 1:3514222/1‑68 (MQ=255) cttGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGc > 1:4058923/1‑39 (MQ=255) cttGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGc > 1:2948929/1‑39 (MQ=255) cttGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGc > 1:2526379/1‑39 (MQ=255) cttGTGATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATGCGCCATGCTGAATc < 1:250179/67‑1 (MQ=255) | AAGATGAATTGAGAGTTCTTCATCCCAGTCATTTTGGCTCTCGTCTGCGGCATCTTCTTGTAATGGAACCCAGCGCCAGGCTGATGGTGTTTTGCCCGGTACCATAATGCGCCATGCTGAATC > W3110S.gb/4178618‑4178740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |