| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,184,522 | C→T | 37.7% | V107I (GTC→ATC) | gspC ← | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,184,522 | 0 | C | T | 37.7% | 110.6 / 125.1 | 114 | V107I (GTC→ATC) | gspC | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (39/32); new base T (19/24); total (58/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
CCCGGCGCTGACTCAAGGGCTTCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCT > W3110S.gb/4184455‑4184590 | cccGGCGCTGACTCAAGGGCTTCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACaa > 1:4267047/1‑70 (MQ=255) gcTGACTCAAGGGCTTCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGt > 1:1598864/1‑71 (MQ=255) gACTCAAGGGCTTCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGtct > 1:4957873/1‑70 (MQ=255) gggCTTCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTc < 1:1583698/71‑1 (MQ=255) gCTTCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGa < 1:593225/71‑1 (MQ=255) gCTTCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGa > 1:3873427/1‑71 (MQ=255) ttCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGAc < 1:4039789/48‑1 (MQ=255) ttCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGAc < 1:752582/48‑1 (MQ=255) cGTTGAGGGAGTATCGCTTTTGTTCTCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGAtgt < 1:1603012/70‑1 (MQ=255) cGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGAtgt > 1:4590866/1‑70 (MQ=255) cGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACaa > 1:4118454/1‑48 (MQ=255) cGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACaa > 1:3068837/1‑48 (MQ=255) cGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACaa > 1:3096046/1‑48 (MQ=255) cGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACaa > 1:1767083/1‑48 (MQ=255) cGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGAtgt < 1:831902/70‑1 (MQ=255) gTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGAtgt < 1:2699357/69‑1 (MQ=255) ttGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCtcat < 1:935842/58‑1 (MQ=255) ttGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAg > 1:3087697/1‑71 (MQ=255) ttGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAg > 1:2666621/1‑71 (MQ=255) ttGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAg < 1:1907239/71‑1 (MQ=255) tGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAg > 1:4494336/1‑70 (MQ=255) tGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAg > 1:4626297/1‑70 (MQ=255) gAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGAt > 1:5027457/1‑64 (MQ=255) aGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTAc < 1:459347/71‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGATaa < 1:3767908/37‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACa < 1:3836132/36‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTcatcat < 1:291735/52‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCg < 1:4076645/55‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCg < 1:2670205/55‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCg < 1:2991187/55‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCg < 1:2020079/55‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATg > 1:3724665/1‑69 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGcc < 1:4006652/71‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGcc < 1:4004499/71‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGc < 1:3284285/70‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGc < 1:711453/70‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGc < 1:2215209/70‑1 (MQ=255) tATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGc > 1:461336/1‑70 (MQ=255) tCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCa > 1:5086930/1‑70 (MQ=255) tCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCAt > 1:3434866/1‑71 (MQ=255) tCGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCAt < 1:780042/71‑1 (MQ=255) tCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCtcat < 1:4531934/47‑1 (MQ=255) tCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGa > 1:5112245/1‑54 (MQ=255) tCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGAt > 1:363982/1‑55 (MQ=255) tCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCAt > 1:4175800/1‑71 (MQ=255) cGCTTTTGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACaa > 1:2984520/1‑64 (MQ=255) gCTTTTGTTCGCTCCCCTCATTAATTAAGACAAaagaa > 1:1156412/1‑38 (MQ=255) gCTTTTGTTCGCTCCCCTCATTAATTAAGACAAaagaa > 1:2084334/1‑38 (MQ=255) gCTTTTGTTCGCTCCCCTCATTAATTAAGACAAaagaa > 1:3792962/1‑38 (MQ=255) gCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCAtt > 1:2607316/1‑70 (MQ=255) gCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCAtt > 1:4254174/1‑70 (MQ=255) gCTTTTATTCGCTCCCCTCATTAATTAAGACAAaagaa > 1:141905/1‑38 (MQ=255) ttttGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGa > 1:1003616/1‑55 (MQ=255) ttttGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGa > 1:1275801/1‑55 (MQ=255) ttGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGAtgt < 1:2685648/51‑1 (MQ=255) ttGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAg < 1:3168979/69‑1 (MQ=255) tGTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGAtgt < 1:2142769/50‑1 (MQ=255) tGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTaa > 1:2594572/1‑67 (MQ=255) gTTCGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTAc < 1:2305510/55‑1 (MQ=255) gTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgcgc < 1:908516/70‑1 (MQ=255) cGCTCCCCTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCtat < 1:2765779/70‑1 (MQ=255) gCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCtata < 1:219855/70‑1 (MQ=255) cccTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCtat < 1:3861347/65‑1 (MQ=255) cccTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgc < 1:2582153/60‑1 (MQ=255) cccTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgc < 1:1744177/60‑1 (MQ=255) ccTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgcgc < 1:3367217/61‑1 (MQ=255) ccTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgcgc < 1:2369760/61‑1 (MQ=255) cTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgctc < 1:2920320/60‑3 (MQ=255) cTCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCt < 1:921086/61‑1 (MQ=255) cTCATTAATTAAGACAAAAGAAGTCTCATCATTCGAtgt < 1:811946/39‑1 (MQ=255) cTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCAt < 1:4636569/53‑1 (MQ=255) cTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgcgc > 1:1055013/1‑60 (MQ=255) cTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgcgc > 1:1120001/1‑60 (MQ=255) cTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgcgc > 1:2411019/1‑60 (MQ=255) cTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgcgc > 1:1845585/1‑60 (MQ=255) cTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCt < 1:237484/71‑1 (MQ=255) tCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGc < 1:4173397/49‑1 (MQ=255) tCATTAATTAAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCta > 1:1901192/1‑61 (MQ=255) cATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGGGCta > 1:1311320/1‑60 (MQ=255) attaattaAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTa > 1:1914813/1‑70 (MQ=255) attaattaAGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAg > 1:52312/1‑71 (MQ=255) attaattaAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAg > 1:1284935/1‑71 (MQ=255) attaattaAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAg > 1:3108216/1‑71 (MQ=255) ttaattaaGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGc < 1:4920847/46‑1 (MQ=255) ttaattaaGACAAAAGAAGTCTCATCATTCGATGTGAGGACAATGc < 1:958788/46‑1 (MQ=255) taattaaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCtata < 1:4544772/59‑1 (MQ=255) aattaaGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCa > 1:4007555/1‑46 (MQ=255) aattaaGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCa > 1:3648255/1‑46 (MQ=255) aattaaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCtata < 1:4177451/58‑1 (MQ=255) aattaaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGgtg > 1:2249784/1‑71 (MQ=255) ttaaGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCa < 1:3686111/44‑1 (MQ=255) ttaaGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCa < 1:3198371/44‑1 (MQ=255) ttaaGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCtat < 1:3107122/55‑1 (MQ=255) ttaaGACAAAAGAAGTCTCATCATTCGATGTGAGTACa < 1:69315/38‑1 (MQ=255) aaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAAt > 1:1702532/1‑38 (MQ=255) aaGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTc > 1:109086/1‑71 (MQ=255) aGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCtat > 1:3021974/1‑52 (MQ=255) aGATAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTc < 1:2375127/70‑1 (MQ=255) aGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAgcgc < 1:74173/49‑1 (MQ=255) aCAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTGTAGAGAGCTTAGGTGTTTCCt > 1:2818704/1‑70 (MQ=255) | CCCGGCGCTGACTCAAGGGCTTCGTTGAGGGAGTATCGCTTTTGTTCGCTCCCCTCATTAATTAAGACAAAAGAAGTCTCATCATTCGATGTGAGTACAATGCCATTAAGCGCTATAGAGAGCTTAGGTGTTTCCT > W3110S.gb/4184455‑4184590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |