| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 345,099 | C→T | 25.0% | A155T (GCC→ACC) | yahN ← | neutral amino‑acid efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 345,099 | 0 | C | T | 25.0% | 97.2 / 31.7 | 52 | A155T (GCC→ACC) | yahN | neutral amino‑acid efflux system |
| Reads supporting (aligned to +/- strand): ref base C (19/20); new base T (7/6); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGT > W3110S.gb/345033‑345162 | gCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTAtt > 1:477671/1‑70 (MQ=255)gCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCAttt < 1:4066861/71‑1 (MQ=255)gCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCAtt > 1:320369/1‑70 (MQ=255)gCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCAtt > 1:2554300/1‑70 (MQ=255) tAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAAt > 1:4200586/1‑68 (MQ=255) aaTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa > 1:479061/1‑66 (MQ=255) aaTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa > 1:867747/1‑66 (MQ=255) aaTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa > 1:2129154/1‑66 (MQ=255) aTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCAt > 1:4073042/1‑61 (MQ=255) aTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCAGGTTGGTGTTTCGGCAt > 1:1597549/1‑61 (MQ=255) aTTGATGCGAGCACAATCCCCGCCAAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTaa > 1:4693091/1‑65 (MQ=255) gATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTg < 1:4190380/70‑1 (MQ=255) gCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATg < 1:3081367/57‑1 (MQ=255) cacaATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCa > 1:4318615/1‑49 (MQ=255) ccccGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGa > 1:1597120/1‑68 (MQ=255) cccAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATaaaaaa > 1:2916828/1‑70 (MQ=255) cAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATaa < 1:4027850/71‑1 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGtt < 1:3235935/38‑1 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaaaa < 1:1431492/48‑1 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaaaa < 1:1406206/48‑1 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATAc < 1:1788206/49‑1 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATAc < 1:4997221/49‑1 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATaaa > 1:1992556/1‑63 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTa > 1:4967696/1‑37 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaaaa > 1:338203/1‑46 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaaaa > 1:2318622/1‑46 (MQ=255) cccATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGtt < 1:2672274/70‑1 (MQ=255) ccATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATa > 1:1338459/1‑40 (MQ=255) tGTTGGTGTTTCTGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTg < 1:2260211/60‑1 (MQ=255) tGTTGGTGTTTCGGCATTTAATGTTACTGAGaaaa > 1:1353266/1‑35 (MQ=255) tGTTGGAGTTTCGGCATTTAATGTTCCTGAGaaaa < 1:1635695/35‑1 (MQ=37) gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATAc < 1:902017/37‑1 (MQ=255) gTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaaaa < 1:808829/52‑1 (MQ=255) gTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaaaa < 1:398524/52‑1 (MQ=255) gTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCggg > 1:3701268/1‑63 (MQ=255) ttGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaaaa > 1:3035014/1‑51 (MQ=255) ttGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:2904485/1‑61 (MQ=255) ttGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg > 1:4761829/1‑61 (MQ=255) ttGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTagagag < 1:3334213/70‑1 (MQ=255) ttGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTaga > 1:4537621/1‑67 (MQ=255) ttGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAgagaga < 1:1508736/71‑1 (MQ=255) tgtTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGc < 1:383542/55‑1 (MQ=255) tgtTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTaga < 1:2432669/63‑1 (MQ=255) tttCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGt < 1:4966714/70‑1 (MQ=255) tttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCg < 1:1646244/54‑1 (MQ=255) tttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGAt < 1:3089128/66‑1 (MQ=255) tttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGt < 1:993403/70‑1 (MQ=255) tttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGt < 1:1031045/70‑1 (MQ=255) tttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGt < 1:2116657/70‑1 (MQ=255) tttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGt < 1:1037025/70‑1 (MQ=255) tCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTa > 1:341397/1‑57 (MQ=255) tCGGCATTTAATGTTACTGAGAAAATACTGATaaa > 1:605034/1‑35 (MQ=255) | GCCAGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGT > W3110S.gb/345033‑345162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |