| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,315,024 | C→T | 37.2% | D213N (GAC→AAC) | alsA ← | fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,315,024 | 0 | C | T | 37.2% | 75.7 / 82.8 | 78 | D213N (GAC→AAC) | alsA | fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (23/26); new base T (11/18); total (34/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01 | |||||||||||
GTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACT > W3110S.gb/4314956‑4315092 | gTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCg > 1:4190653/1‑70 (MQ=255) aTTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCaaa < 1:4502569/71‑1 (MQ=255) aTTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCaaa < 1:2592975/71‑1 (MQ=255) aTTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCaa < 1:3828097/70‑1 (MQ=255) cacaTCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGc > 1:3146316/1‑71 (MQ=255) aTCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGaa > 1:4659668/1‑71 (MQ=255) gCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAAtt < 1:1551547/70‑1 (MQ=255) gCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAAtt < 1:360499/70‑1 (MQ=255) cTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTc < 1:3359521/71‑1 (MQ=255) ttACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCa < 1:253379/55‑1 (MQ=255) tACTATGCCGCTGCAAAGGCTGCTGCCGTCTTTCATCACCGTATAGCGGTc > 1:3539264/1‑51 (MQ=255) tACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTc > 1:3722027/1‑51 (MQ=255) tACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCg > 1:2870136/1‑71 (MQ=255) cTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCg < 1:3999199/61‑1 (MQ=255) cTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCg < 1:366963/61‑1 (MQ=255) tATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCa < 1:4002221/51‑1 (MQ=255) tATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCa < 1:1347143/51‑1 (MQ=255) aTGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCaa > 1:740296/1‑71 (MQ=255) aTGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCaa > 1:4851526/1‑71 (MQ=255) tGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAAc > 1:3269276/1‑71 (MQ=255) gCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACt > 1:4073757/1‑71 (MQ=255) ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACtt < 1:4866374/71‑1 (MQ=255) ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACtt < 1:1779482/71‑1 (MQ=255) ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACt < 1:226814/70‑1 (MQ=255) ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACt < 1:4883502/70‑1 (MQ=255) ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACt < 1:2933137/70‑1 (MQ=255) ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACtt < 1:5047699/71‑1 (MQ=255) aaCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGc > 1:4567961/1‑67 (MQ=255) aCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGa > 1:1828607/1‑48 (MQ=255) aCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGc > 1:2826735/1‑56 (MQ=255) aCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCgaga > 1:4893665/1‑70 (MQ=255) aCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGat < 1:770176/71‑1 (MQ=255) cGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGata < 1:3128912/71‑1 (MQ=255) cGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGata < 1:2827193/71‑1 (MQ=255) gctgcCGTCTTTCATCACCGTATAGCGGTTGCaaa < 1:2883864/35‑1 (MQ=255) gctgcCGTCTTTCATCACCCTATAGCGGTCGCAAATACGGCGAATTTCCGCCa > 1:3938359/1‑53 (MQ=255) ctgcCGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGAc < 1:3652921/71‑1 (MQ=255) ctgcCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGAc < 1:5092246/71‑1 (MQ=255) ccGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACtt < 1:4298377/53‑1 (MQ=255) ccGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACg < 1:3565143/69‑1 (MQ=255) ccGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa < 1:1568795/70‑1 (MQ=255) ccGTCTTTCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa < 1:3987794/70‑1 (MQ=255) gTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCgag > 1:130791/1‑58 (MQ=255) gTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACGTATGCgag > 1:387334/1‑58 (MQ=255) tCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGc < 1:1039853/54‑1 (MQ=255) tttCATCACCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGata > 1:4005025/1‑58 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGTGAATTTCCGCCAACt < 1:78713/47‑1 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAAttt > 1:5101891/1‑38 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAAt > 1:37633/1‑36 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGc > 1:4036225/1‑42 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACt < 1:1816966/47‑1 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTa > 1:1803304/1‑49 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATg > 1:1980928/1‑51 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGc < 1:4698511/52‑1 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGa > 1:3922502/1‑65 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATg > 1:3783899/1‑67 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCg > 1:861574/1‑71 (MQ=255) tttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCg > 1:1050931/1‑71 (MQ=255) catcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGt < 1:4077947/69‑1 (MQ=255) atcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACtt > 1:2176071/1‑44 (MQ=255) tcaCCGTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACg < 1:3622388/59‑1 (MQ=255) aCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCctc > 1:3147114/1‑71 (MQ=255) ccGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCctct > 1:1250254/1‑71 (MQ=255) cGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCtt < 1:4615998/71‑1 (MQ=255) cGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGCCCGTACCCTCtt < 1:3028500/71‑1 (MQ=255) gTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACGCTCttt < 1:661869/71‑1 (MQ=255) gTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCttt < 1:1972120/71‑1 (MQ=255) gTATAGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCttt < 1:4096995/71‑1 (MQ=255) tataGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTa > 1:4279335/1‑71 (MQ=255) tataGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTa > 1:4263539/1‑71 (MQ=255) tataGCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTa > 1:456778/1‑71 (MQ=255) tataGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTa < 1:2919553/71‑1 (MQ=255) taGCGGTTGCAAATACGGCGAATTTCCGCCAACTTa > 1:3245369/1‑36 (MQ=255) gCGGTTGCAAATACGGCGAATTTCCGCCAACTTAt > 1:3742320/1‑35 (MQ=255) gCGGTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGc < 1:2190630/70‑1 (MQ=255) gCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAg < 1:1785648/46‑1 (MQ=255) gCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAg < 1:1779752/46‑1 (MQ=255) ggTTGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAAc > 1:4708750/1‑71 (MQ=255) ggTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAAc < 1:181291/71‑1 (MQ=255) gTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACt < 1:4821323/71‑1 (MQ=255) | GTCATTTGACACATCGCTTACTATGCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACT > W3110S.gb/4314956‑4315092 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |