| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,324,790 | T→C | 25.7% | Y185C (TAC→TGC) | phnI ← | carbon‑phosphorus lyase complex subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,324,790 | 0 | T | C | 25.7% | 54.7 / 21.5 | 35 | Y185C (TAC→TGC) | phnI | carbon‑phosphorus lyase complex subunit |
| Reads supporting (aligned to +/- strand): ref base T (16/10); new base C (4/5); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01 | |||||||||||
CCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCT > W3110S.gb/4324722‑4324859 | ccagcgccagcAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCaa < 1:3458429/71‑1 (MQ=255) gccagcAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCAAACcg > 1:2416004/1‑70 (MQ=255) tAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCAAACCGGCGGCGTGCg > 1:1863517/1‑71 (MQ=255) aGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCAAACCGGCGGCGTGCgg > 1:3837029/1‑71 (MQ=255) tcgtcgCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACcgg > 1:1466353/1‑56 (MQ=255) tcgtcgCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACcgg > 1:2648223/1‑56 (MQ=255) tcgtcgCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGAt > 1:3332954/1‑71 (MQ=255) cgtcgCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATg > 1:3550335/1‑71 (MQ=255) gccgcGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCAAACCGGCGGCGTGCGGGTGATGTCa < 1:3504081/70‑1 (MQ=255) gccgcGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCAAACCGGCGGCGTGCGGGTGATGTCAt < 1:2246403/71‑1 (MQ=255) gccgcGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCAAACCGGCGGCGTGCGGGTGATGTCAt < 1:3972899/71‑1 (MQ=255) cgcATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTg > 1:571063/1‑60 (MQ=255) cgcATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGCAAACCGGCGGCGTGCGGGTGATGTCATCCgg < 1:1521368/71‑1 (MQ=255) aTCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCtg < 1:2364810/71‑1 (MQ=255) cAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCtgtg > 1:3800845/1‑71 (MQ=255) cAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCtgtg > 1:4835021/1‑71 (MQ=255) aaCTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACcgg > 1:3378441/1‑41 (MQ=255) aaCTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACcgg > 1:4234985/1‑41 (MQ=255) aaCTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACcgg > 1:710787/1‑41 (MQ=255) aaCTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACcgg > 1:1917793/1‑41 (MQ=255) aaCTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACcg > 1:1921548/1‑40 (MQ=255) aaCTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTgc > 1:744379/1‑71 (MQ=255) ctgctgCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGc > 1:179112/1‑47 (MQ=255) ctgctgCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTgcgc > 1:173200/1‑71 (MQ=255) cAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGGGATGTCATCCGGCTGTGCGCCGCTa < 1:4336560/70‑1 (MQ=255) aCGGGAGGAGCGTGAGCAGGGGCAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCct > 1:712406/1‑71 (MQ=255) gggAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATg < 1:3042748/44‑1 (MQ=255) gggAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATg < 1:3036975/44‑1 (MQ=255) gggAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATg < 1:2214473/44‑1 (MQ=255) gggAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATg < 1:3986956/44‑1 (MQ=255) aggagCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCa < 1:4396471/71‑1 (MQ=255) gCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACt < 1:4228258/71‑1 (MQ=255) gTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTc < 1:3373501/71‑1 (MQ=255) aGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGc < 1:2010922/70‑1 (MQ=255) gTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCt > 1:4995690/1‑71 (MQ=255) | CCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCT > W3110S.gb/4324722‑4324859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |