| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,328,660 | C→T | 29.5% | V125I (GTC→ATC) | phnD ← | phosphonate/organophosphate ester transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,328,660 | 0 | C | T | 29.5% | 66.3 / 32.5 | 44 | V125I (GTC→ATC) | phnD | phosphonate/organophosphate ester transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (17/14); new base T (4/9); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTG > W3110S.gb/4328592‑4328727 | gCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTtgatga > 1:3260146/1‑71 (MQ=255)gCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTtgatga > 1:1462736/1‑71 (MQ=255) ccAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTATTTGTTgatgat > 1:4318030/1‑71 (MQ=255) aaaGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCa < 1:2803797/71‑1 (MQ=255) aaaGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCa < 1:2382478/71‑1 (MQ=255) ggTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAaca < 1:805213/71‑1 (MQ=255) agaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCt > 1:2204488/1‑71 (MQ=255) gaTCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTc > 1:3175648/1‑71 (MQ=255) tCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTc > 1:1488356/1‑69 (MQ=255) cTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTc > 1:3721082/1‑68 (MQ=255) cTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAg > 1:4511205/1‑71 (MQ=255) tttCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAg > 1:605254/1‑70 (MQ=255) tCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGt < 1:903949/69‑1 (MQ=255) ccGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTaa < 1:4793348/70‑1 (MQ=255) agcagATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCa < 1:2177592/46‑1 (MQ=255) aTCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATc < 1:2440887/40‑1 (MQ=255) aTCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTc < 1:871542/49‑1 (MQ=255) aTCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGt < 1:680036/53‑1 (MQ=255) gcGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCa < 1:2216703/39‑1 (MQ=255) cGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGa > 1:3489456/1‑36 (MQ=255) cGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGa > 1:5030963/1‑36 (MQ=255) cGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGa > 1:91929/1‑36 (MQ=255) agTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAAc > 1:1886357/2‑54 (MQ=255) ttCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCcgccgc < 1:2503075/71‑1 (MQ=255) cAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCTATCCATCCGCCGCGa > 1:1144634/1‑71 (MQ=255) aGGTTGTTGATCGGACTGGCTTTGGTGACGAGCAACACGCTCCAGTAACGCGGCGATCCATCCGCcgcg < 1:1171654/69‑1 (MQ=255) tgttgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCgg > 1:4558540/1‑49 (MQ=255) tgttgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatcca > 1:4032144/1‑56 (MQ=255) tgttgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatcca > 1:5068451/1‑56 (MQ=255) ttgATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTc < 1:3297434/69‑1 (MQ=255) ttgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGat < 1:380168/51‑1 (MQ=255) ttgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGat < 1:5130291/51‑1 (MQ=255) ttgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGat < 1:4342134/51‑1 (MQ=255) tgATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatcc < 1:3667251/52‑1 (MQ=255) aTCTGACTGTCTTTGTTGACGATCAACACGCTCCAGTAAc > 1:1445481/1‑40 (MQ=255) aTCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatccat > 1:4494711/1‑52 (MQ=255) aTCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatccat > 1:1295281/1‑52 (MQ=255) aTCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGatccat > 1:3275923/1‑52 (MQ=255) tCGGTCTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCg < 1:1975107/71‑1 (MQ=255) tCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCg < 1:2033235/71‑1 (MQ=255) aCTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAg < 1:3872786/70‑1 (MQ=255) gTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGAcc < 1:3114789/55‑1 (MQ=255) tCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTg > 1:74522/1‑71 (MQ=255) tttGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATccgc < 1:1522796/45‑1 (MQ=255) tttGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGAc > 1:1894236/1‑66 (MQ=255) tgttgACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGcc < 1:4308366/70‑1 (MQ=255) tgACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTg > 1:4490562/1‑71 (MQ=255) | GCCAAAGGTGAGATCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCATTG > W3110S.gb/4328592‑4328727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |