| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,333,478 | G→A | 17.0% | R555H (CGC→CAC) | yjdA → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,333,478 | 0 | G | A | 17.0% | 132.2 / 18.3 | 53 | R555H (CGC→CAC) | yjdA | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base G (19/25); new base A (5/4); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01 | |||||||||||
AGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTT > W3110S.gb/4333408‑4333541 | aGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCg > 1:2300275/1‑71 (MQ=255) gcgACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGccc < 1:2130448/70‑1 (MQ=255) cTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAtt > 1:2763569/1‑70 (MQ=255) cTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCAtt > 1:1888966/1‑70 (MQ=255) tACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAg > 1:4644549/1‑70 (MQ=255) tACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAg < 1:1250859/70‑1 (MQ=255) cGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGca < 1:2390878/70‑1 (MQ=255) cGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGca < 1:1438039/70‑1 (MQ=255) tGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCAccc < 1:4340466/58‑1 (MQ=255) tGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCAccc < 1:1313358/58‑1 (MQ=255) cGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGt > 1:1032994/1‑71 (MQ=255) ttttGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCACCCCATTGAGCAACAAGTc > 1:2168450/1‑70 (MQ=255) ttttGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCACCCCATTGAGCAACAAGTCa < 1:1710817/71‑1 (MQ=255) tttGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCa > 1:1613061/1‑70 (MQ=255) ttGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCACCCCATTGAGCAACAAGTCaa < 1:4848692/70‑1 (MQ=255) gCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCaaaa < 1:4299209/70‑1 (MQ=255) ccGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaac < 1:1847624/60‑1 (MQ=255) gAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGcaacaa > 1:1557411/1‑60 (MQ=255) tGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATgg > 1:331129/1‑65 (MQ=255) aaTCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGc < 1:2783726/71‑1 (MQ=255) cACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGaa > 1:4902506/1‑61 (MQ=255) cACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAg < 1:3888496/70‑1 (MQ=255) tttGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAgg > 1:4443970/1‑70 (MQ=255) tGCCGGGTGTTAACCGATGTTATTCACCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTc > 1:1925575/1‑65 (MQ=255) tGCCGGGTGTTAACCGATGTTATTCACCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTc > 1:3409827/1‑65 (MQ=255) gCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGtt > 1:4475358/1‑70 (MQ=255) gCCGGGTGTTAACCGATGTTATTCGCCCCATTGAG‑AACAAGTCAAAATGGAATTGAGCGAg > 1:3776013/1‑61 (MQ=255) gCCGGGTGTTAACCGATGTTATTCGACCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGttt > 1:4113655/1‑71 (MQ=255) ccGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGtt < 1:1910932/69‑1 (MQ=255) tgtTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGa > 1:2594353/1‑55 (MQ=255) tgtTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCt < 1:4729941/71‑1 (MQ=255) ttAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGa < 1:4666803/44‑1 (MQ=255) ttAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGc < 1:3843451/51‑1 (MQ=255) aaCCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGt > 1:1956084/1‑71 (MQ=255) aaCCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGt > 1:736940/1‑71 (MQ=255) aCCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGtt < 1:2153063/71‑1 (MQ=255) ccGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATgg < 1:2163082/39‑1 (MQ=255) cGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTc < 1:1256809/71‑1 (MQ=255) cGATGTTATTCACCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGttt > 1:3262442/1‑70 (MQ=255) gATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGaa > 1:1278510/1‑39 (MQ=255) gATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCg < 1:4968785/59‑1 (MQ=255) aTGTTATTCGCCCCATTGAGCAACAAGTCAAAATg < 1:3865567/35‑1 (MQ=255) tGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAggg > 1:5054240/1‑52 (MQ=255) tGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAggg > 1:933769/1‑52 (MQ=255) tGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCAtt < 1:1435116/71‑1 (MQ=255) tGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTATCGTcc < 1:11298/60‑1 (MQ=255) gTTATTCGCCCCATTGAGCAACAAGTCAAAATGGa > 1:5111823/1‑35 (MQ=255) ttATTCGCCCCATTGAGCAACAAGTCAAAATGGaa < 1:3590165/35‑1 (MQ=255) ttATTCGCCCCATTGAGCAACAAGTCAAAATGGaa < 1:3191043/35‑1 (MQ=255) ttATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCa < 1:4732805/67‑1 (MQ=255) ttATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCa < 1:3262913/67‑1 (MQ=255) ttATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCAttt < 1:3930402/70‑1 (MQ=255) ttATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCAttt < 1:3409759/70‑1 (MQ=255) aTTCACCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCt > 1:424986/1‑57 (MQ=255) | AGTCGCGACTTTACGCTGCGTTTTGCCGAGCTTGAATCCACCCTTTGCCGGGTGTTAACCGATGTTATTCGCCCCATTGAGCAACAAGTCAAAATGGAATTGAGCGAGTCAGGGTTTCGTCCTGGGTTTCATTT > W3110S.gb/4333408‑4333541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |